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2021 Good Quality Ngs Workflow - Whole transcriptome sequencing – Illumina – Biomarker

Service Details

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Which has a positive and progressive attitude to customer's desire, our corporation constantly improves our merchandise quality to satisfy the desires of consumers and further focuses on safety, reliability, environmental demands, and innovation of Rarefaction Curve , Metabarcoding Cost , Hifi Sequencing Service , As an experienced group we also accept custom-made orders. The main intention of our firm is to build up a satisfying memory for all consumers, and set up a long-term win-win small business connection.

2021 Good Quality Ngs Workflow - Whole transcriptome sequencing – Illumina – Biomarker Detail:

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Contamination	Amount
OD260/280≥1.7-2.5； OD260/230≥0.5-2.5；	For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation	Limited or no protein or DNA contamination shown on gel.	Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.

Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)

Recommended Sample Delivery

Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:
1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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Our company promises all buyers of the first-class products and solutions as well as most satisfying post-sale support. We warmly welcome our regular and new shoppers to join us for 2021 Good Quality Ngs Workflow - Whole transcriptome sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Sydney , Czech , Bogota , We hope we can establish long-term cooperation with all of the customers. And hope we can improve competitiveness and achieve the win-win situation together with the customers. We sincerely welcome the customers from all over the world to contact us for anything you need!

Next Gen Sequence