Products

2021 High quality Hifi Sequencing Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker

Service Details

Feedback (2)

To get the stage of realizing dreams of our employees! To build a happier, more united and much more skilled crew! To reach a mutual benefit of our prospects, suppliers, the society and ourselves for Nuclear Genome , Gene Structure , Shotgun Sequencing Cost , We sincerely welcome overseas customers to consult for the long-term cooperation and the mutual development.We strongly believe that we can do better and better.

2021 High quality Hifi Sequencing Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:

Advantages

Sample Requirements and Delivery

Library	Sequencing strategy	Data recommended	Quality Control
Poly A enriched	Illumina PE150	6 Gb	Q30≥85%

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel.	For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

logo_01

Experiment design

logo_02

Sample delivery

logo_03

RNA extraction

logo_04

Library construction

logo_05

Sequencing

logo_06

Data analysis

logo_07

After-sale services

Product detail pictures:

Related Product Guide:

continue to improve, to ensure product quality in line with market and customer standard requirements. Our company has a quality assurance system have been established for 2021 High quality Hifi Sequencing Provider - Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Hungary , Paris , Vancouver , We have established long-term, stable and good business relationships with many manufacturers and wholesalers around the world. Currently, we are looking forward to even greater cooperation with overseas customers based on mutual benefits. Please feel free to contact us for more details.

De Novo Genome