2021 New Style Metagenome Sequencing - small RNA

2021 New Style Metagenome Sequencing - small RNA – Biomarker

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2021 New Style Metagenome Sequencing - small RNA – Biomarker Detail:

Bioinformatics

Raw data quality control

Common and unique sequence among samples

Small RNA classification

Alignment with reference genome (+/- strands)

Genome-wide smallRNA distribution genes

Known miRNA identification

Small RNA annotation (miRBASE, RepBASE, Rfam, SILVA, GtRNAdb, etc.)

miRNA base perference

miRNA base editing

miRNA family

miRNA quantification

Correlation analysis

miRNA differential expression analysis

Hierarchical clustering on DE-miRNAs

miRNA target gene prediction

Function annotation on DE-miRNA target genes

GO and KEGG enrichment on DE-miRNA target genes

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