Big Discount Insertion And Deletion - Full-length mRNA sequencing -Nanopore – Biomarker
Big Discount Insertion And Deletion - Full-length mRNA sequencing -Nanopore – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Alternative splicing
ü Expression quantification in gene level and isoform level
ü Differential expression analysis
ü Function annotation and enrichment (DEGs and DETs)
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel. | For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥40 ng/μl;Total ≥ 1 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
The company keeps to the operation concept "scientific management, high quality and efficiency primacy, customer supreme for Big Discount Insertion And Deletion - Full-length mRNA sequencing -Nanopore – Biomarker , The product will supply to all over the world, such as: Brazil , Paris , Uruguay , Customer's satisfaction is always our quest, creating value for customers is always our duty, a long term mutual-beneficial business relationship is what we are doing for. We are an absolutely reliable partner for you in China. Of course, other services, like consulting, can be offered too.
By Andrew Forrest
from Zurich
- 2018.12.14 15:26
The product manager is a very hot and professional person, we have a pleasant conversation, and finally we reached a consensus agreement.
By Michelle
from Bolivia
- 2018.06.05 13:10
