Big discounting De Novo Genome Assembly - De novo Full-length Transcriptome sequencing -PacBio – Biomarker
Big discounting De Novo Genome Assembly - De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Sequence structure
ü Expression Quantification
ü Function Annotation
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Amount | Total ≥ 3 μg; Conc. ≥ 300 ng/μl; Volumn ≥ 10 μl |
| Integrity | For plants: RIN ≥ 7.5; For animals: RIN ≥ 8; 28S/18S ≥ 1.0; Limited or no baseline elevation |
| Purity | OD260/280 ≥ 1.8; OD260/230 ≥ 1.0; Clear peak at 260 nm |
| Note | Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level. |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 – 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
It can be our duty to satisfy your preferences and successfully serve you. Your pleasure is our best reward. We have been looking forward to the go to for joint expansion for Big discounting De Novo Genome Assembly - De novo Full-length Transcriptome sequencing -PacBio – Biomarker , The product will supply to all over the world, such as: Estonia , Lithuania , Nepal , We are seeking the chances to meet all the friends from both at home and abroad for the win-win cooperation. We sincerely hope to have long-term cooperation with all of you on the bases of mutual benefit and common development.
By Irma
from Bahamas
- 2018.02.08 16:45
We have been engaged in this industry for many years, we appreciate the work attitude and production capacity of the company, this is a reputable and professional manufacturer.
By Emily
from Denver
- 2017.10.27 12:12
