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China OEM Next Generation Sequencing Service - Non-Reference based mRNA sequencing-Illumina – Biomarker

Service Details

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We always continually provide you with the most conscientious customer service, and the widest variety of designs and styles with finest materials. These efforts include the availability of customized designs with speed and dispatch for Single Nucleus Rna Seq , Bioinformatics Service , Rna Extraction , For further inquires please do not hesitate to contact us. Thank you - Your support continuously inspires us.

China OEM Next Generation Sequencing Service - Non-Reference based mRNA sequencing-Illumina – Biomarker Detail:

Features

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Contamination	Amount
OD260/280≥1.7-2.5； OD260/230≥0.5-2.5；	For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation	Limited or no protein or DNA contamination shown on gel.	Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended.

Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)

Recommended Sample Delivery

Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:
1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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