Products

DNA/RNA Sequencing – Nanopore Sequencer

ONT sequencing is a single molecule real-time electrical signal sequencing technology based on nanopores, the sequencing principle of each platform is the same. Double-stranded DNA/RNA will bind to nanoporous protein embedded in the biofilm and unwinding under the lead of motor protein, under the action of voltage difference from both sides of the biofilm, DNA/RNA strands pass through the nanopore channel protein at a certain rate. Due to the differences of the chemical properties of the different bases on the DNA/RNA strand, when a single base or DNA molecule passes through the nanopore channel, it will cause the change of different electrical signals. By detecting and corresponding to these signals, the corresponding base types can be calculated, and the real-time detection of the sequence can be completed.

Service Details

Demo Result

Service Details Features

Platform	Library Size	Theoretical Data Yield (Per Cell)	Single-base Accuracy	Applications
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Cell	85-92%	SV calling, De novo, Full-length sequencing, Iso-Seq, Gene annotation, Detection of DNA methylation

Service Advantages

● Over 5 years’ experience on PacBio sequencing platform with thousands of closed projects with various species.
● BMKGENE is an official partner of Oxford Nanopore, with dual platform RNA/DNA certification.
● There are mainstream models of sequencers with complete equipment and sufficient sequencing throughput.
● Based on the Nanopore platform, more than 10 animal and plant Denovo researches have been published in internationally renowned journals.

Sample Requirements

Sample Type

Amount

Concentrtion(Qubit ®)

Volume

Purity

Others

Genomic DNA

Depend on Data Requirement

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5；

Clear peak at 260 nm，no contaminations

Concentration needs to be measured by Qubit and Qubit/Nanopore ≤ 2

Total RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5；no contaminations

RIN value ≥7.5

Service Workflow

Sample preparation

Library construction

Sequencing

Data analysis

Project delivery

Previous: Illumina pre-made libraries

Next: Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Data Quality Assessment of DNA Sample

Table 1. Statistics on clean data.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	cleanN50Len	cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9