Europe style for Metagenomics And Microbiome - Eukaryotic mRNA sequencing-Illumina – Biomarker
Europe style for Metagenomics And Microbiome - Eukaryotic mRNA sequencing-Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
| Library | Sequencing strategy | Data recommended | Quality Control |
| Poly A enriched | Illumina PE150 |
6 Gb |
Q30≥85% |
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. | For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
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By Mignon from Croatia - 2017.10.23 10:29
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