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Factory making Strand-Specific Rna-Seq - Eukaryotic mRNA sequencing – Illumina – Biomarker


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Factory making Strand-Specific Rna-Seq - Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:

Advantages

Sample Requirements and Delivery

Library Sequencing strategy Data recommended Quality Control
Poly A enriched Illumina PE150

6 Gb

Q30≥85%

Sample Requirements:

Nucleotides:

Purity Integrity Amount
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Microorganism: Mass ≥ 1 g

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:

  1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
  2. RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services


Product detail pictures:

Factory making Strand-Specific Rna-Seq -
 Eukaryotic mRNA sequencing – Illumina – Biomarker detail pictures

Factory making Strand-Specific Rna-Seq -
 Eukaryotic mRNA sequencing – Illumina – Biomarker detail pictures

Factory making Strand-Specific Rna-Seq -
 Eukaryotic mRNA sequencing – Illumina – Biomarker detail pictures

Factory making Strand-Specific Rna-Seq -
 Eukaryotic mRNA sequencing – Illumina – Biomarker detail pictures

Factory making Strand-Specific Rna-Seq -
 Eukaryotic mRNA sequencing – Illumina – Biomarker detail pictures

Factory making Strand-Specific Rna-Seq -
 Eukaryotic mRNA sequencing – Illumina – Biomarker detail pictures


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  • This manufacturers not only respected our choice and requirements, but also gave us a lot of good suggestions, ultimately, we successfully completed the procurement tasks.
    5 Stars By Cindy from Ukraine - 2018.12.28 15:18
    The company's products can meet our diverse needs, and the price is cheap, the most important is that the quality is also very nice.
    5 Stars By Chris from Colombia - 2018.05.13 17:00

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