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Factory making Strand-Specific Rna-Seq - Fungal Genome – Biomarker

Service Details

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Factory making Strand-Specific Rna-Seq - Fungal Genome – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

üRaw data quality control

üGenome assembly

üGenome component analysis

üGene function annotation

üComparative genomic analysis

Sample Requirements and Delivery

Sample Requirements:

For DNA extracts:

Sample Type	Amount	Concentration	Purity
DNA extracts	＞ 3 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

For tissue samples:

Sample type

Recommended sample treatment

Sample storage and shipment

Unicellular Fungus

Observe yeast under microscope and collect them in their exponential phase

Transfer the culture (containing approx. 3-4.5e9 cells) into a 1.5 or 2 ml eppendorf. (Keep on ice)

Centrifuge the tube for 1 min at 14000 g to collect bacteria and remove the supernatant carefully

Seal the tube and freeze the bacteria in liquid nitrogen for at least 30 min. Store the tube in -80 ℃ fridge.

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Macro Fungus

Tissue in actively growing phase is recommended.

Rinse the tissue with endotoxin-free water, then 70% ethonal.

Save the sample in cryo-tubes.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

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Bioinformatics Service