Factory source Transcriptome - Full-length mRNA sequencing -Nanopore – Biomarker
Factory source Transcriptome - Full-length mRNA sequencing -Nanopore – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Alternative splicing
ü Expression quantification in gene level and isoform level
ü Differential expression analysis
ü Function annotation and enrichment (DEGs and DETs)
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel. | For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥40 ng/μl;Total ≥ 1 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
continue on to further improve, to make sure product top quality in line with market and consumer standard requirements. Our firm has a excellent assurance program have already been established for Factory source Transcriptome - Full-length mRNA sequencing -Nanopore – Biomarker , The product will supply to all over the world, such as: Buenos Aires, Grenada, Ghana, We welcome customers from all over the world come to discuss business. We supply high quality products, reasonable prices and good services. We hope to sincerely build business relationships with customers from at home and abroad, jointly striving for a resplendent tomorrow.
By Mike from Swedish - 2018.09.21 11:44
The company's products very well, we have purchased and cooperated many times, fair price and assured quality, in short, this is a trustworthy company!
By Victor Yanushkevich from Uganda - 2017.11.12 12:31
