Factory Supply Rna Sequencing Workflow - Nanopore-based metagenome sequencing – Biomarker
Factory Supply Rna Sequencing Workflow - Nanopore-based metagenome sequencing – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data quality control
ü Metagenome assembly
ü Non-redundant gene set and annotation
ü Species diversity analysis
ü Genetic function diversity analysis
ü Inter-group analysis
ü Association analysis against experimental factors
Sample Requirements and Delivery
Sample requirements and delivery
Sample Requirements:
For DNA extracts:
|
Sample Type |
Amount |
Concentration |
Purity |
|
DNA extracts |
＞ 30 ng |
＞ 1 ng/μl |
OD260/280= 1.6-2.5 |
For environmental samples:
|
Sample type |
Recommended sampling procedure |
|
Soil |
Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation. |
|
Feces |
Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation. |
|
Intestinal contents |
Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes. |
|
Sludge |
Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation |
|
Waterbody |
For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube. |
|
Skin |
Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube. |
Recommended Sample Delivery
Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
We retain bettering and perfecting our goods and service. At the same time, we perform actively to do research and enhancement for Factory Supply Rna Sequencing Workflow - Nanopore-based metagenome sequencing – Biomarker , The product will supply to all over the world, such as: Poland , Sao Paulo , Luxembourg , With the best technological support, we have tailored our website for the best user experience and kept in mind your ease of shopping. we ensure that the best reaches you at your doorstep, in the shortest possible time and with the help of our efficient logistical partners i.e DHL and UPS. We promise quality, living by the motto of promising only what we can deliver.
By Erica
from Estonia
- 2018.12.28 15:18
This is the first business after our company establish, products and services are very satisfying, we have a good start, we hope to cooperate continuous in the future!
By Fiona
from Japan
- 2018.12.22 12:52
get a quote
Write your message here and send it to us
