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Factory wholesale Shotgun Sequencing -
De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Sequence structure
ü Expression Quantification
ü Function Annotation

Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Amount |
Total ≥ 3 μg; Conc. ≥ 300 ng/μl; Volumn ≥ 10 μl |
| Integrity |
For plants: RIN ≥ 7.5; For animals: RIN ≥ 8; 28S/18S ≥ 1.0; Limited or no baseline elevation |
| Purity |
OD260/280 ≥ 1.8; OD260/230 ≥ 1.0; Clear peak at 260 nm |
| Note |
Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level. |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 – 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services
Product detail pictures:
Related Product Guide:
Our mission will be to grow to be an innovative supplier of high-tech digital and communication devices by giving worth added design and style, world-class production, and service capabilities for
Factory wholesale Shotgun Sequencing -
De novo Full-length Transcriptome sequencing -PacBio – Biomarker , The product will supply to all over the world, such as: Hyderabad
, Morocco
, Romania
, In order to make more people know our products and to enlarge our market, we have devoted a lot of attention to technical innovations and improvement, as well as replacement of equipment. Last but not the least, we also pay more attention to training our managerial personnel, technicians and workers in planned way.