circRNA sequencing-Ilumina
Siffofin
● Ragewar rRNA yana biye da shirye-shiryen ɗakin karatu na jagora, yana ba da damar jeri bayanan da ya keɓanta.
● Ayyukan aiki na bioinformatic yana ba da damar tsinkayar circRNA da ƙididdige magana
Amfanin Sabis
●Ƙarin cikakkun ɗakunan karatu na RNA:muna amfani da raguwar rRNA a maimakon raguwar RNA na layi a cikin shirye-shiryenmu na farko na ɗakin karatu, muna tabbatar da cewa bayanan da aka tsara sun haɗa ba kawai circRNA ba har ma mRNA da lncRNA, yana ba da damar nazarin haɗin gwiwa akan waɗannan bayanan.
●Binciken zaɓi na hanyoyin sadarwar RNA (ceRNA) masu gasa: samar da zurfafa fahimta game da hanyoyin sarrafa salon salula
●Ƙwararren Ƙwararru: tare da rikodin waƙa na sarrafa samfuran sama da 20,000 a BMK, haɓaka nau'ikan samfura iri-iri da ayyukan lncRNA, ƙungiyarmu tana kawo ƙwarewar ƙwarewa ga kowane aiki.
●Tsananin Kula da Inganci: muna aiwatar da mahimman abubuwan sarrafawa a duk matakai, daga samfurin da shirye-shiryen ɗakin karatu zuwa jerin abubuwa da bioinformatics.Wannan saka idanu mai kyau yana tabbatar da isar da sakamako mai inganci akai-akai.
● Tallafin Bayan Talla: Alƙawarinmu ya wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan-sayar.A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.
Samfuran Bukatun da Bayarwa
| Laburare | Dandalin | Bayanan da aka ba da shawarar | Bayanan Bayani na QC |
| Poly A ya wadata | Farashin PE150 | 16-20 GB | Q30≥85% |
Samfuran Bukatun:
Nucleotides:
| Conc.(ng/μl) | Adadin (μg) | Tsafta | Mutunci |
| ≥ 100 | ≥ 0.5 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Iyakance ko babu furotin ko gurɓataccen DNA da aka nuna akan gel. | Don tsire-tsire: RIN≥6.5; Na dabbobi: RIN≥7.0; 5.0≥28S/18S≥1.0; iyakance ko babu hawan tushe |
● Shuke-shuke:
Tushen, kara ko petal: 450 MG
Leaf ko iri: 300 MG
'Ya'yan itace: 1.2 g
● Dabbobi:
Zuciya ko hanji: 450 MG
Viscera ko Brain: 240 MG
tsoka: 600 MG
Kasusuwa, Gashi ko Fata: 1.5g
● Arthropods:
Kwari: 9g
Crustacea: 450 MG
● Cikakken jini:2 bututu
● Kwayoyin: 106 Kwayoyin
● Magani da Plasmaku: 6 ml
Isar da Samfurin Nasiha
Kwantena: 2 ml bututu centrifuge (Ba a ba da shawarar foil tin ba)
Alamar samfur: Ƙungiya+ kwafi misali A1, A2, A3;B1, B2, B3......
Jirgin ruwa:
1. Busasshen ƙanƙara: Ana buƙatar samfurori a cikin jaka kuma a binne su a bushe-kankara.
2. RNAstable tubes: Ana iya bushe samfuran RNA a cikin bututun daidaitawar RNA (misali RNAstable®) kuma a tura su cikin zafin jiki.
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
RNA cirewa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Bioinformatics
Hasashen circRNA: rarrabawar chromosomal
Bambance-bambancen circRNAs - Makircin mai aman wuta
Bambance-bambancen circRNAs - tari na matsayi
Haɓaka aiki na kwayoyin halittar circRNA
Bincika ci gaban bincike ta hanyar BMKGene' circRNA sabis na jerin ayyukan ta hanyar tarin wallafe-wallafe.
Wang, X. et al.(2021) 'CPSF4 tana daidaita samuwar circRNA da microRNA mediated gene silencing a hepatocellular carcinoma', Oncogene 2021 40:25, 40(25), shafi 4338-4351.doi: 10.1038/s41388-021-01867-6.
Xia, K. et al.(2023) 'X oo-amsa kwafin yana bayyana rawar da'irar RNA133 ke da ita a cikin juriya na cututtuka ta hanyar daidaita maganganun OsARAB a cikin shinkafa', Binciken Halittar Halittu, 5 (1), shafi 1-14.doi: 10.1186/S42483-023-00188-8/FIGURES/6.
Y, H. da al.(2023) 'CPSF3 yana daidaita ma'auni na madauwari da kwafi na layi a cikin carcinoma na hepatocellular'.doi: 10.21203/RS.3.RS-2418311/V1.
Zhang, Y. et al.(2023) 'Cikakken kimantawa na circRNAs a cikin cirrhotic cardiomyopathy kafin da bayan dashen hanta', International Immunopharmacology, 114, p.109495. doi: 10.1016/J.INTIMP.2022.109495.
