Kayayyaki

Fasahar jerin bayanai ta Illumina, wadda ta dogara ne akan Sequencing by Synthesis (SBS), wata sabuwar fasaha ce ta NGS da aka yarda da ita a duk duniya, wacce ke da alhakin samar da sama da kashi 90% na bayanan jerin bayanai na duniya. Ka'idar SBS ta ƙunshi ɗaukar hotunan masu ƙarewa masu haske yayin da ake ƙara kowane dNTP, sannan a raba su don ba da damar haɗa tushe na gaba. Tare da duk dNTP guda huɗu masu ɗaurewa masu juyawa waɗanda ke cikin kowane zagayen jerin bayanai, gasa ta halitta tana rage son zuciya a cikin tsarin haɗa bayanai. Wannan fasaha mai amfani tana tallafawa ɗakunan karatu guda ɗaya da na ƙarshe, suna ba da dama ga aikace-aikacen genomic iri-iri. Ikon sarrafa bayanai na Illumina da daidaiton sa sun sanya shi a matsayin ginshiƙi a cikin binciken genomics, suna ba masana kimiyya damar warware matsalolin kwayoyin halitta tare da cikakkun bayanai da inganci marasa misaltuwa.

Sabis ɗinmu na tsara ɗakin karatu da aka riga aka yi yana ba abokan ciniki damar shirya ɗakunan karatu na tsara littattafai daga tushe daban-daban (mRNA, cikakken genome, amplicon, ɗakunan karatu na 10x, da sauransu). Bayan haka, ana iya aika waɗannan ɗakunan karatu zuwa cibiyoyin tsara littattafai don kula da inganci da tsara su a dandamalin Illumina.