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Kayayyaki

Illumina da BGI

Fasahar sequencing na Illumina, dangane da Sequencing by Synthesis (SBS), ƙirƙira ce ta NGS ta duniya wacce ke da alhakin samar da sama da kashi 90% na bayanan jeri na duniya.Ka'idar SBS ta ƙunshi ɗaukar hoto mai lakabi mai jujjuyawa kamar yadda aka ƙara kowane dNTP, kuma daga baya an raba shi don ba da damar haɗa tushe na gaba.Tare da duk dNTPs guda huɗu masu jujjuyawa masu ɗaure da ke akwai a cikin kowane zagayowar zagayowar, gasa ta yanayi tana rage son haɗawa.Wannan fasaha mai jujjuyawar tana goyan bayan dakunan karatu guda-ɗaya da masu haɗa-zuwa-ƙarshe, suna ba da kewayon aikace-aikacen kwayoyin halitta.Illumina sequencing's high-throughput capabilities and madaidaicin sanya shi a matsayin ginshiƙi a cikin binciken kimiyyar halittu, ƙarfafa masana kimiyya don buɗe ɓarna na kwayoyin halitta tare da cikakkun bayanai da inganci.

DNBSEQ, wanda BGI ta haɓaka, wata sabuwar fasaha ce ta NGS wacce ta yi nasarar rage raguwar farashin jeri da haɓaka kayan aiki.Shirye-shiryen dakunan karatu na DNBSEQ ya ƙunshi rarrabuwar DNA, shirye-shiryen ssDNA da haɓaka da'ira don samun DNA nanoballs (DNB).Ana ɗora waɗannan a kan ƙaƙƙarfan wuri kuma daga baya ana yin su ta hanyar haɗakar Binciken-Anchor Synthesis (cPAS).

Sabis ɗin jerin laburaren da aka yi da aka riga aka yi yana sauƙaƙe abokan ciniki wajen shirya dakunan karatu daga mabambanta (mRNA, gabaɗayan kwayoyin halitta, amplicon, da sauransu).Daga baya, waɗannan ɗakunan karatu za a iya jigilar su zuwa cibiyoyin jerin abubuwan mu don kula da inganci da tsari a dandamalin Illumina ko BGI.


Cikakkun Sabis

Sakamakon Demo

Siffofin

Dandalin:Illumina NovaSeq 6000, NovaSeq, HiSeq X Ten da BGI-DNB-T7

Hanyoyin jeri:PE50, PE100, PE150, PE250

Kula da ingancin ɗakunan karatu kafin tsarawa

Jeri isar da bayanai da QC:isar da rahoton QC da ɗanyen bayanai a cikin tsarin fastq bayan lalatawa da tacewa Q30 yana karantawa.

 

Amfanin Sabis

Ƙwararren sabis na Sequencing:abokin ciniki na iya zaɓar jeri ta hanya, tantanin halitta mai gudana ko adadin bayanai.

Juyawa na dandamali:Ana iya canja wurin ɗakunan karatu na DNB zuwa dandamali na Illumina

Ƙwarewa mai zurfi akan dandamalin jerin abubuwan Illumina:tare da dubban rufaffiyar ayyukan tare da nau'o'in nau'i daban-daban. 

Isar da rahoto na QC:tare da ma'auni masu inganci, daidaiton bayanai da aikin gaba ɗaya na aikin jeri.

Babban tsarin jeri:tare da ɗan gajeren lokacin juyawa.

Tsananin Kula da Inganci: muna aiwatar da ƙaƙƙarfan buƙatun QC don tabbatar da isar da sakamako mai inganci akai-akai.

Misalin Bukatun*

Matsakaicin Sashe na Layi

Adadin Bayanai (X)

Hankali (qPCR/nM)

Ƙarar

X ≤ 50 GB

≥ 2 nM

≥ 20 ml

50 Gb ≤ X <100 Gb

≥ 3 nM

≥ 20 ml

X ≥ 100 Gb

≥ 4 nM

≥ 20 ml

Hanya Daya (Illumina)

Dandalin

Hankali (qPCR/nM)

Ƙarar

HiSeq X Ten

≥ 2 nM

≥ 20 ml

NovaSeq 6000 SP

≥ 1 nM

≥ 25 ml

NovaSeq 6000 S4

≥ 1.5 nM

≥ 25 ml

NovaSeq X

≥ 1.5 nM

≥ 25 ml

BGI-DNBSEQ-T7

≥ 1.5 nM

≥ 25 ml

 Baya ga maida hankali da jimlar adadin, ana kuma buƙatar ƙirar kololuwar da ta dace.

 

Da fatan za a tuntuɓe mu idan samfuran ku ba su cika buƙatun kayan farawa ba.

Gudun Aikin Sabis

samfurin shiri

Kula da ingancin ɗakin karatu

Jeri

Jeri

Binciken bayanai

Kula da ingancin bayanai

Misalin QC

Isar da aikin


  • Na baya:
  • Na gaba:

  • Rahoton Laburare QC

    Ana ba da rahoto kan ingancin ɗakin karatu kafin tsarawa, tantance adadin ɗakin karatu, da rarrabuwa.

     

    Tsarin rahoton QC

     

    Tebur 1. Ƙididdiga akan jerin bayanai.

    Samfurin ID

    BMKID

    Raw yana karantawa

    Raw Data (bp)

    Tsabtace karantawa (%)

    Q20(%)

    Q30(%)

    GC(%)

    C_01

    BMK_01

    22,870,120

    6,861,036,000

    96.48

    99.14

    94.85

    36.67

    C_02

    BMK_02

    14,717,867

    4,415,360,100

    96.00

    98.95

    93.89

    37.08

    Hoto 1. Rarraba inganci tare da karantawa a cikin kowane samfurin

    A9

    Hoto 2. Tushen rarraba abun ciki

    A10

    Hoto 3. Rarraba abubuwan da aka karanta a cikin jerin bayanai

    A11

     

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