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Chromatin Immunoprecipitation Sequencing (ChiP-seq)
ChIP-Seq yana ba da faffadan bayanan kwayoyin halittar DNA don gyare-gyaren tarihi, abubuwan rubutawa, da sauran sunadaran da ke da alaƙa da DNA.Yana haɗa zaɓi na chromatin immuno-hazo (ChiP) don dawo da takamaiman hadaddun furotin-DNA, tare da ikon tsarin tsarawa na gaba (NGS) don babban tsarin aiwatarwa na DNA da aka samu.Bugu da ƙari, saboda an dawo da rukunin furotin-DNA daga sel masu rai, ana iya kwatanta wuraren ɗaure a nau'ikan tantanin halitta da kyallen takarda, ko ƙarƙashin yanayi daban-daban.Aikace-aikace sun bambanta daga ƙa'idodin rubutun zuwa hanyoyin haɓakawa zuwa hanyoyin cututtuka da kuma bayan.
Platform: Illumina NovaSeq 6000
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Gabaɗayan jerin abubuwan genome bisulfite
DNA methylation a matsayi na biyar a cikin cytosine (5-mC) yana da tasiri mai mahimmanci akan maganganun kwayoyin halitta da aikin salula.An haɗu da tsarin methylation mara kyau tare da yanayi da cututtuka da yawa, kamar ciwon daji.WGBS ya zama ma'auni na zinariya don nazarin methylation mai fa'ida ga kwayoyin halitta a ƙudurin tushe guda ɗaya.
Platform: Illumina NovaSeq6000
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Ƙididdiga don Canjawa-Sarfafa Chromatin tare da Babban Sabis na Sabis (ATAC-seq)
ATAC-seq wata hanya ce mai mahimmanci don nazarin damar samun damar chromatin na kwayoyin halitta, wanda ke da mahimmanci ga kula da epigenetic na duniya na maganganun kwayoyin halitta.Ana shigar da adaftan jeri cikin buɗaɗɗen yankuna na chromatin ta hanyar transposase mai ƙarfi Tn5.Bayan haɓakawa na PCR, ana gina ɗakin karatu na jerin abubuwa.Ana iya samun duk wuraren buɗewar chromatin a ƙarƙashin takamaiman yanayin lokacin sarari, ba kawai iyakance ga wuraren ɗaure na abin da aka rubuta ba, ko wani yanki da aka gyara na tarihi.
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Rage Matsayin Bisulfite Sequencing (RRBS)
Binciken methylation na DNA ya kasance batu mai zafi a cikin bincike na cututtuka, kuma yana da alaƙa da alaƙa da maganganun kwayoyin halitta da halaye masu kama-da-wane.RRBS hanya ce mai kyau, inganci da tattalin arziki don binciken DNA methylation.Haɓakawa na masu haɓakawa da yankunan tsibirin CpG ta hanyar ɓarnawar enzymatic (Msp I), haɗe tare da jerin Bisulfite, yana ba da babban ƙudurin gano methylation na DNA.
Platform: Illumina NovaSeq 6000
