China Eukaryotic mRNA sequencing-Ilumina factory da kuma masana'antun |BMK

Eukaryotic mRNA sequencing-Illumina

Tsarin mRNA yana ba da damar yin bayanin duk mRNAs da aka rubuta daga sel a ƙarƙashin takamaiman yanayi.Fasaha ce mai ƙarfi don bayyana bayanan bayanan kwayoyin halitta, tsarin kwayoyin halitta da hanyoyin kwayoyin wasu hanyoyin nazarin halittu.Har zuwa yau, an yi amfani da jeri na mRNA a cikin bincike na asali, bincike na asibiti, haɓaka magunguna, da sauransu.

Platform: Illumina NovaSeq 6000

Tuntube Mu

Cikakkun Sabis

Bioinformatics

Sakamakon Demo

Amfani

ØKware sosai: Sama da samfuran 200,000 an sarrafa su a cikin BMK waɗanda ke rufe nau'ikan samfura daban-daban, gami da al'adun tantanin halitta, nama, ruwan jiki, da sauransu.

ØTsananin kula da ingancin inganci: Mahimman abubuwan kula da inganci ta duk matakan da suka haɗa da shirye-shiryen samfurin, shirye-shiryen ɗakin karatu, jerin abubuwa da bioinformatics suna ƙarƙashin kulawa sosai don sadar da sakamako mai inganci.

ØMabuɗin bayanai da yawa akwai don bayanin aiki da nazarin haɓakawa don cika burin bincike iri-iri.

ØBayan-sayar da sabis: Bayan-sayar da sabis na aiki na tsawon watanni 3 bayan kammala aikin, gami da bin ayyukan ayyuka, harbin matsala, Q&A sakamakon, da sauransu.

Samfuran Bukatun da Bayarwa

Laburare	Dabarun jeri	An ba da shawarar bayanai	Kula da inganci
Poly A ya wadata	Farashin PE150	6 gb	Q30≥85%

Samfuran Bukatun:

Nucleotides:

Tsafta	Mutunci	Adadin
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Iyakance ko babu furotin ko gurɓataccen DNA da aka nuna akan gel.	Don tsire-tsire: RIN≥6.5; Na dabbobi: RIN≥7; 28S/18S≥1.0; iyaka ko babu haɓakar asali	Conc.≥30 ng/ml;Matsakaicin ≥ 10 μl;Jimlar ≥ 1.5 μg

Nama: Nauyi(bushe):≥1 g
* Don nama ƙasa da 5 MG, muna ba da shawarar aika samfurin nama daskararre (a cikin ruwa nitrogen).

Dakatar da salula:Ƙididdiga ta salula = 3×10⁶- 1 × 10⁷
*Muna ba da shawarar jigilar daskararrun cell lysate.Idan wannan tantanin halitta ya ƙidaya ƙasa da 5 × 105, ana ba da shawarar daskararre mai walƙiya a cikin ruwa nitrogen, wanda ya fi dacewa don hakar micro.

Samfuran jini:Girman ≥1 ml

Microorganism:Masa ≥ 1 g

Isar da Samfurin Nasiha

Kwantena: 2 ml bututu centrifuge (Ba a ba da shawarar foil tin ba)

Alamar samfur: Ƙungiya+ kwafi misali A1, A2, A3;B1, B2, B3......

Jigila:

Busasshen ƙanƙara: Ana buƙatar ɗaukar samfuran a cikin jakunkuna kuma a binne su cikin busasshiyar kankara.
RNAstable tubes: Ana iya bushe samfuran RNA a cikin bututun tabbatar da RNA (misali RNAstable®) kuma a tura su cikin zafin jiki.