Eukaryotic mRNA Sequencing-Illumina

Tsarin mRNA yana ba da damar yin bayanin duk mRNAs da aka rubuta daga sel a ƙarƙashin takamaiman yanayi.Fasaha ce mai ƙarfi don bayyana bayanan bayanan kwayoyin halitta, tsarin kwayoyin halitta da hanyoyin kwayoyin wasu hanyoyin nazarin halittu.Ya zuwa yau, an yi amfani da jeri na mRNA a cikin bincike na asali, bincike na asibiti, haɓaka magunguna, da sauransu.

Platform: Illumina NovaSeq Platform

Tuntube Mu

Cikakkun Sabis

Bioinformatics

Sakamakon Demo

Amfani

● Kwarewa sosai: Sama da samfuran 200,000 an sarrafa su a cikin BMK waɗanda ke rufe nau'ikan samfuri daban-daban, gami da al'adun tantanin halitta, nama, ruwan jiki, da sauransu kuma sama da ayyukan mRNA-Seq sama da 7,000 da aka rufe waɗanda ke rufe wuraren bincike daban-daban.

● Tsarin kula da inganci mai mahimmanci: Mahimman matakan kula da inganci ta duk matakai ciki har da shirye-shiryen samfurin, shirye-shiryen ɗakin karatu, jerin abubuwa da bioinformatics suna ƙarƙashin kulawa mai zurfi don samar da sakamako mai kyau.

● Rubutun bayanai da yawa akwai don bayanin aiki da nazarin haɓakawa don cika burin bincike daban-daban.

● Bayan-sayar da sabis: Bayan-sayar da sabis na aiki na tsawon watanni 3 bayan kammala aikin, gami da bin diddigin ayyukan, harbin matsala, Q&A sakamakon, da sauransu.

Samfuran Bukatun da Bayarwa

Laburare	Dabarun jeri	An ba da shawarar bayanai	Kula da inganci
Poly A ya wadata	Farashin PE150	6 gb	Q30≥85%

Samfuran Bukatun:

Nucleotides:

Conc.(ng/μl)

Adadin (μg)

Tsafta

Mutunci

≥ 20

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Iyakance ko babu furotin ko gurɓataccen DNA da aka nuna akan gel.

Don tsire-tsire: RIN≥6.5;

Na dabbobi: RIN≥7.0;

5.0≥28S/18S≥1.0;

iyakance ko babu hawan tushe

Nama: Nauyi(bushe):≥1 g
* Don nama ƙasa da 5 MG, muna ba da shawarar aika samfurin nama daskararre (a cikin ruwa nitrogen).

Dakatar da salula:Ƙididdigar salula = 3×10⁶- 1 × 10⁷
*Muna ba da shawarar jigilar daskararrun cell lysate.Idan wannan tantanin halitta ya ƙidaya ƙasa da 5 × 105.

Samfuran jini:Girman ≥1 ml

Microorganism:Masa ≥ 1 g

Isar da Samfurin Nasiha

Kwantena: 2 ml bututu centrifuge (Ba a ba da shawarar foil tin ba)

Alamar samfur: Ƙungiya+ kwafi misali A1, A2, A3;B1, B2, B3......

Jirgin ruwa:

Busasshen ƙanƙara: Ana buƙatar ɗaukar samfurori a cikin jakunkuna kuma a binne su a bushe-kankara.
RNAstable tubes: Ana iya bushe samfuran RNA a bututun daidaitawar RNA (misali RNAstable®) kuma a tura su cikin zafin jiki.