Tsarin mRNA Mai Tsawon-Cikakken Tsawon-Nanopore
Siffofi
● Kama mRNA na poly-A sannan a biyo bayan hada cDNA da kuma shirya ɗakin karatu
● Jerin cikakkun rubuce-rubucen
● Binciken bioinformatic bisa ga daidaitawa zuwa kwayar halittar da aka ambata
● Binciken bioinformatic ya ƙunshi ba kawai bayyana a matakin kwayar halitta da isoform ba, har ma da nazarin lncRNA, haɗuwar kwayoyin halitta, poly-adenylation da tsarin kwayoyin halitta.
Fa'idodin Sabis
●Ƙididdige yawan magana a matakin isoform: yana ba da damar yin cikakken bayani da daidaito kan magana, yana bayyana canjin da za a iya ɓoyewa yayin nazarin dukkan bayyanar kwayar halitta
●Rage Bukatun Bayanai:Idan aka kwatanta da Tsarin Tsarin Gaggawa na Gaba (NGS), tsarin jerin bayanai na Nanopore yana nuna ƙarancin buƙatun bayanai, wanda ke ba da damar daidaita matakan cikar bayanin kwayoyin halitta tare da ƙananan bayanai.
●Ingantaccen daidaito na ƙididdige magana: duka a matakin kwayoyin halitta da kuma matakin isoform
●Gano ƙarin bayanan transcriptomic: madadin polyadenylation, kwayoyin halittar haɗin gwiwa da lcnRNA da kwayoyin halittar da aka yi niyya
●Ƙwarewa Mai Zurfi: Ƙungiyarmu tana kawo ƙwarewa mai yawa ga kowane aiki, bayan kammala sama da ayyukan Nanopore guda 850 na cikakken zane-zane da kuma sarrafa sama da samfura 8,000.
●Tallafin Bayan Tallace-tallace: alƙawarinmu ya wuce kammala aikin tare da tsawon watanni 3 na sabis bayan sayarwa. A wannan lokacin, muna ba da bibiyar aikin, taimakon magance matsaloli, da zaman tambayoyi da amsoshi don magance duk wata tambaya da ta shafi sakamakon.
Samfurin Bukatun da Isarwa
| Laburare | Dabarun tsara tsari | Bayanan da aka ba da shawarar | Sarrafa Inganci |
| An wadatar da Poly A | Nanopore PromethION 48 | 6/12 Gb | Matsakaicin maki mai inganci: Q10 |
Bukatun Samfura:
Nukliyoyin Nukliyoyin:
| Conc.(ng/μl) | Adadi (μg) | Tsarkaka | Mutunci |
| ≥ 100 | ≥ 1.0 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 An nuna ƙarancin gurɓataccen furotin ko DNA akan gel ɗin. | Ga shuke-shuke: RIN≥7.0; Ga dabbobi: RIN≥7.5; 5.0≥28S/18S≥1.0; iyaka ko babu matakin tushe |
● Shuke-shuke:
Tushe, Tushe ko Furen Fure: 450 MG
Ganye ko Iri: 300 MG
'Ya'yan itace: 1.2 g
● Dabbobi:
Zuciya ko Hanji: 300 MG
Jijiyoyin ciki ko kwakwalwa: 240 MG
Tsoka: 450 MG
Kashi, Gashi ko Fata: 1g
● Arthropods:
Kwari: 6g
Crustacea: 300 MG
● Jini gaba ɗaya: Bututu 1
● Kwayoyin halitta: 106 ƙwayoyin halitta
Isarwa Samfurin da Aka Ba da Shawara
Akwati: Bututun centrifuge na 2 ml (Ba a ba da shawarar a saka tin foil ba)
Lakabi samfurin: Rukuni+kwafi misali A1, A2, A3; B1, B2, B3.
Jigilar kaya:
1. Busasshen Kankara: Ana buƙatar a saka samfuran a cikin jakunkuna a binne su a cikin busasshen kankara.
2. Bututun RNA masu karko: Ana iya busar da samfuran RNA a cikin bututun daidaita RNA (misali RNAstable®) kuma a aika su a zafin ɗaki.
Tsarin Aikin Sabis
Nukliyoyin Nukliyoyin:
Isarwa samfurin
Gina ɗakin karatu
Jerin abubuwa
Nazarin bayanai
Ayyukan bayan-sayarwa
Tsarin Aikin Sabis
Nama:
Tsarin gwaji
Isarwa samfurin
Fitar da RNA
Gina ɗakin karatu
Jerin abubuwa
Nazarin bayanai
Ayyukan bayan-sayarwa
● Sarrafa bayanai marasa inganci
● Gano rubutun
● Madadin haɗawa
● Ƙimar bayyanawa a matakin kwayar halitta da matakin isoform
● Binciken bayyana ra'ayi daban-daban
● Bayanin aiki da haɓaka shi (DEGs da DETs)
Nazarin haɗin maɓalli na madadin
Madadin Binciken Polyadenylation (APA)
Hasashen lncRNA
Bayanin sabbin kwayoyin halitta
Rarraba DETs
Cibiyoyin Sadarwar Protein-Protein a cikin DEGs
Bincika ci gaban da ayyukan BMKGene's Nanopore mai cikakken tsayin mRNA suka samar ta hanyar tarin wallafe-wallafe da aka tsara.
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