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Tsarin Shuka/Dabbobi De novo Genome Sequencing
Da novosequencing na nufin gina wani nau'i 'dukan kwayoyin halitta ta hanyar amfani da fasahohin jeri, misali PacBio, Nanopore, NGS, da dai sauransu, in babu kwayar halitta.Haɓakawa mai ban sha'awa a cikin tsayin karantawa na fasahar tsararrun ƙarni na uku ya kawo sabbin damammaki a haɗa hadaddun kwayoyin halitta, kamar waɗanda ke da babban heterozygosity, babban rabo na yankuna masu maimaitawa, polyploids, da sauransu. warware abubuwa masu maimaitawa, yankuna tare da abubuwan da ke cikin GC mara kyau da sauran yankuna masu rikitarwa.
Platform: PacBio Sequel II / Nanopore PromethION P48 / Illumina NovaSeq6000
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Hi-C tushen Genome Assembly
Hi-C wata hanya ce da aka ƙera don ɗaukar daidaitawar chromosome ta hanyar haɗa ma'amala ta tushen kusanci da babban tsarin aiwatarwa.An yi imanin ƙarfin waɗannan hulɗar yana da alaƙa mara kyau tare da nisa ta jiki akan chromosomes.Don haka, bayanan Hi-C na iya jagorantar tari, oda da daidaita jerin abubuwan da aka haɗa a cikin daftarin kwayoyin halitta da kuma rataya waɗanda suke kan takamaiman adadin chromosomes.Wannan fasaha tana ba da ƙarfin taro na matakin chromosome ba tare da taswirar tushen yawan jama'a ba.Kowane kwayar halitta guda ɗaya yana buƙatar Hi-C.
Platform: Illumina NovaSeq6000 / DNBSEQ
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Juyin Halitta
Juyin Halitta sabis ne mai cike da jeri wanda aka ƙera don samar da cikakkiyar fassarar bayanan juyin halitta na kayan da aka bayar dangane da bambance-bambancen kwayoyin halitta, gami da SNPs, InDels, SVs da CNVs.Yana ba da duk mahimman bincike da ake buƙata don kwatanta sauye-sauyen juyin halitta da sifofin kwayoyin halitta na yawan jama'a, kamar tsarin yawan jama'a, bambancin kwayoyin halitta, dangantakar phylogeny, da dai sauransu. Har ila yau yana ƙunshe da bincike game da kwararar kwayoyin halitta, wanda ke ba da damar ƙididdige yawan girman yawan jama'a, lokacin bambanta.
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Kwatancen Genomics
Kwatankwacin ilimin genomics a zahiri yana nufin kwatanta cikakken jerin kwayoyin halitta da sigar nau'ikan nau'ikan nau'ikan nau'ikan.Wannan horon yana nufin bayyanar da juyin halittar nau'in, aikin kwayoyin halitta, tsarin sarrafa kwayoyin halitta a matakin kwayoyin halitta ta hanyar gano tsarin tsari da abubuwan da aka kiyaye ko aka bambanta a cikin nau'ikan nau'ikan daban-daban.Nazari na kwatankwacin genomics na yau da kullun ya haɗa da nazari a cikin dangin jinsin halitta, haɓakar juyin halitta, kwafin kwayoyin halitta gabaɗaya, matsa lamba, da sauransu.
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Bulked Segregant bincike
Bulked Segregant analysis (BSA) wata dabara ce da aka yi amfani da ita don gano alamomin kwayoyin halitta masu alaƙa da sauri.Babban tsarin aiki na BSA ya ƙunshi zaɓin ƙungiyoyi biyu na mutane masu ƙima da ƙima, haɗa DNA na duk mutane don samar da mafi yawan DNA guda biyu, yana gano jerin bambance-bambance tsakanin tafkuna biyu.An yi amfani da wannan fasaha sosai wajen gano alamomin kwayoyin halitta masu alaƙa da alaƙa da kwayoyin da aka yi niyya a cikin kwayoyin halittar shuka/dabba.
