Taro na Genome Mai Tushen Hi-C
Hi-C wata hanya ce da aka tsara don kama tsarin chromosome ta hanyar haɗa hulɗar da ta dogara da kusanci da kuma jerin abubuwan da aka samar da su sosai. Ana kyautata zaton ƙarfin waɗannan hulɗar yana da alaƙa mara kyau da nisan jiki akan chromosomes. Saboda haka, ana amfani da bayanan Hi-C don jagorantar tattarawa, tsarawa, da kuma daidaita jerin abubuwan da aka haɗa a cikin daftarin kwayar halitta da kuma haɗa su akan wani adadin chromosomes. Wannan fasaha tana ƙarfafa tarin kwayoyin halitta na matakin chromosome idan babu taswirar kwayoyin halitta bisa ga yawan jama'a. Kowane kwayar halitta ɗaya tana buƙatar Hi-C.
Fasallolin Sabis
● Jerin sunayen Illumina NovaSeq tare da PE150.
● Sabis yana buƙatar samfuran nama, maimakon ƙwayoyin nucleic acid da aka cire, don haɗa haɗin formaldehyde da kuma kiyaye hulɗar DNA-protein.
● Gwajin Hi-C ya ƙunshi takaitawa da gyara ƙarshen mannewa da biotin, sannan a zagaya ƙarshen da ya ƙunsa yayin da ake kiyaye hulɗar. Sannan a ja DNA ɗin ƙasa da ƙwallo streptavidin sannan a tsarkake shi don shirya ɗakin karatu na gaba.
Fa'idodin Sabis
Bayani game da Hi-C
(Lieberman-Aiden E da sauransu,Kimiyya, 2009)
●Kawar da Bukatar Bayanan Yawan Halitta:Hi-C yana maye gurbin mahimman bayanai da ake buƙata don toshewar contig.
●Babban yawan alamar alama:wanda ke haifar da babban rabon anga mai hadewa sama da kashi 90%.
●Kwarewa da bayanan wallafe-wallafe masu zurfi:BMKGene yana da ƙwarewa sosai a fannin fiye da shari'o'i 2000 na Hi-C Genome Assembly daga nau'ikan halittu 1000 daban-daban da kuma haƙƙin mallaka daban-daban. Sama da shari'o'i 200 da aka buga suna da tasirin tarin sama da 2000.
●Ƙungiyar ƙwararrun bioinformatics:Tare da haƙƙin mallaka na cikin gida da haƙƙin mallaka na software don gwaje-gwajen Hi-C da nazarin bayanai, software ɗin da aka haɓaka da kansa yana ba da damar motsawa, juyawa, sokewa, da sake yin aiki da hannu.
●Tallafin Bayan Talla:Alƙawarinmu ya wuce kammala aikin da tsawon watanni 3 bayan an sayar da shi. A wannan lokacin, muna ba da shawarwari kan yadda za a bi diddigin aikin, taimakawa wajen magance matsaloli, da kuma zaman tambayoyi da amsoshi don magance duk wata tambaya da ta shafi sakamakon.
●Cikakken Bayani: muna amfani da bayanai da yawa don yin bayani game da kwayoyin halittar da ke da bambance-bambancen da aka gano da kuma yin nazarin wadatarwa mai dacewa, yana ba da haske kan ayyukan bincike da yawa.
Bayanin Sabis
| Shirye-shiryen ɗakin karatu | Dabarun tsara tsari | An ba da shawarar fitar da bayanai | Kula da inganci |
| Laburare na Hi-C | Illumina NovaSeq PE150 | 100x | Q30 ≥ 85% |
Samfurin Bukatun
| Nama | Adadin da ake buƙata |
| Viscera na Dabbobi | ≥ 2 g |
| Tsokar Dabbobi | |
| Jinin Dabbobi Masu Shayarwa | ≥ 2 mL |
| Jinin Kaji/Kifi | |
| Shuke-Sabon Ganyen Ganyayyaki | ≥ 3 g |
| Kwayoyin Halitta Masu Al'adu | ≥ 1x107 |
| Kwari | ≥ 2 g |
Tsarin Aikin Sabis
Tsarin gwaji
Isarwa samfurin
Gina ɗakin karatu
Jerin abubuwa
Nazarin bayanai
Ayyukan bayan-sayarwa
1) Takaddun bayanai na asali (QC)
2) Laburaren Hi-C QC: kimanta hulɗar Hi-C mai inganci
3) Haɗawar Hi-C: tattara ƙwayoyin cuta a cikin ƙungiyoyi, sannan a bi tsarin contig a cikin kowace ƙungiya da kuma sanya yanayin contig
4) Kimantawa Mai Kyau (Hi-C)
QC Library na Hi-C - kimantawa na haɗin gwiwar Hi-C masu inganci
Majalisar Hi-C - ƙididdiga
Kimantawa bayan haɗawa - taswirar zafi na ƙarfin sigina tsakanin kwandon shara
Bincika ci gaban da ayyukan haɗa Hi-C na BMKGene suka samar ta hanyar tarin wallafe-wallafe da aka tsara.
Tian, T. da sauransu (2023) 'Tarin kwayoyin halitta da rarraba kwayoyin halitta na wani sanannen ƙwayar masara mai jure fari', Nature Genetics 2023 55:3, 55(3), shafi na 496–506. doi: 10.1038/s41588-023-01297-y.
Wang, ZL da sauransu (2020) 'Taron Tsarin Halittar Kudan zuma na Asiya', Frontiers in Genetics, 11, shafi na 524140. doi: 10.3389/FGENE.2020.00279/BIBTEX.
Zhang, F. et al. (2023) 'Bayyana juyin halittar alkaloid na tropane ta hanyar nazarin kwayoyin halitta guda biyu a cikin dangin Solanaceae', Nature Communications 2023 14:1, 14(1), shafi na 1–18. doi: 10.1038/s41467-023-37133-4.
Zhang, X. da sauransu (2020) 'Kwayoyin Halitta na Itacen Banyan da Kudan zuma Masu Gurɓata Yana Ba da Bayani Game da Haɗakar Fig-Wasp', Cell, 183(4), shafi na 875-889.e17. doi: 10.1016/J.CELL.2020.09.043
