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Kayayyaki

Tsarin Metagenomic (NGS)

Metagenome yana nufin tarin jimillar kayan gado na gaurayawan al'umman halittu, kamar muhalli metagenome, metagenome na mutum, da sauransu. Ya ƙunshi kwayoyin halittar halittu masu rai da waɗanda ba za a iya nomawa ba.Tsarin metagenomic kayan aiki ne na kwayoyin halitta da ake amfani da su don nazarin gaurayen kayan halitta da aka samo daga samfuran muhalli, wanda ke ba da cikakkun bayanai game da bambancin nau'in da yawa, tsarin yawan jama'a, dangantakar halittu, kwayoyin halittar aiki da hanyar sadarwa tare da abubuwan muhalli.

Dandalin:Illumina NovaSeq6000


Cikakkun Sabis

Sakamakon Demo

Nazarin Harka

Amfanin Sabis

ØWarewa da noma-kyauta don bayyanar da ƙananan ƙwayoyin cuta

ØBabban ƙuduri a gano ƙananan nau'in nau'in nau'in yawa a cikin samfuran muhalli

ØMa'anar "meta-" ta haɗa dukkan siffofi na halitta a matakin aiki, nau'in nau'in jinsin da jinsin halitta, wanda ke nuna ra'ayi mai mahimmanci wanda ya fi kusa da gaskiya.

ØBMK yana tara ƙwarewa mai yawa a cikin nau'ikan samfuri daban-daban tare da samfuran sama da 10,000 da aka sarrafa.

Ƙayyadaddun Sabis

JeriDandalin

Laburare

Nasihar yawan amfanin bayanai

Kiyasin lokacin juyawa

Illumina NovaSeq 6000

PE250

50K/100K/300K Tags

Kwanaki 30

Binciken bioinformatics

üRaw data ingancin iko

üMetagenome taro

üSaitin kwayoyin halitta marasa ƙarfi da annotation

üNazari iri-iri

üBinciken bambancin aikin kwayoyin halitta

üBinciken Inter-group

üBinciken ƙungiyoyi akan abubuwan gwaji

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Samfuran Bukatun da Bayarwa

Samfuran Bukatun:

DominCire DNA:

Nau'in Misali

Adadin

Hankali

Tsafta

Cire DNA

ku 30ng

1 ng/l

OD260/280= 1.6-2.5

Don samfuran muhalli:

Nau'in samfurin

Hanyar samfurin da aka ba da shawarar

Ƙasa

Adadin samfur: kimanin.5 g; ku.Ya kamata a cire ragowar abin da ya bushe daga saman;Niƙa manyan guda kuma ku wuce ta 2 mm tace;Aliquot samfurori a cikin bakararre EP-tube ko cyrotube don ajiya.

Najasa

Adadin samfur: kimanin.5 g; ku.Tattara da liquot samfurori a cikin bakararre EP-tube ko cryotube don ajiyar wuri.

Abubuwan ciki na hanji

Ana buƙatar sarrafa samfuran ƙarƙashin yanayin aseptic.Wanke nama da aka tattara tare da PBS;Centrifuge PBS kuma tattara hazo a cikin EP-tubes.

Lalacewa

Adadin samfur: kimanin.5 g; ku.Tattara da liquot samfurin sludge a cikin bakararre EP-tube ko cryotube don ajiyar wuri

Ruwan ruwa

Don samfurin tare da iyakataccen adadin ƙwayoyin cuta, kamar ruwan famfo, ruwan rijiya, da sauransu, Tattara ruwa aƙalla 1 L kuma ku wuce ta 0.22 μm tace don wadatar da ƙananan ƙwayoyin cuta akan membrane.Ajiye membrane a cikin bututu bakararre.

Fatar jiki

A hankali a goge saman fata tare da bakararre auduga ko ruwan fida da sanya shi cikin bututu maras kyau.

Isar da Samfurin Nasiha

Daskare samfuran a cikin ruwa nitrogen na sa'o'i 3-4 kuma adana a cikin ruwa nitrogen ko -80 digiri zuwa ajiyar dogon lokaci.Ana buƙatar jigilar samfuri tare da bushe-kankara.

Gudun Aikin Sabis

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Samfurin bayarwa

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Gina ɗakin karatu

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Jeri

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Binciken bayanai

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Bayan-sayar da sabis


  • Na baya:
  • Na gaba:

  • 1.Histogram: Rarraba nau'ikan

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    2.Ayyukan kwayoyin halitta annotate zuwa KEGG hanyoyin rayuwa

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    3.Heat taswira: Ayyuka daban-daban dangane da yawan jinsin dangi54.Circos na CARD kwayoyin juriya

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    BMK Case

    Yawaitar kwayoyin juriya na ƙwayoyin cuta da ƙwayoyin cuta tare da ci gaba da tushen ƙasa-mangrove

    Buga:Jaridar Abubuwan Matsala, 2021

    Dabarun tsarawa:

    Materials: DNA tsantsa guda hudu na tushen mangrove samfurori masu alaƙa: ƙasa marar shuka, rhizosphere, episphere da sassan endosphere
    Platform: Illumina HiSeq 2500
    Manufa: Metagenome
    16S rRNA gene V3-V4 yankin

    Sakamako masu mahimmanci

    Matsakaicin metagenomic da bayanan metabarcoding akan tushen ƙasa na ci gaba da tsiron mangrove an sarrafa su don yin nazarin yaduwar ƙwayoyin ƙwayoyin ƙwayoyin cuta (ARGs) daga ƙasa zuwa tsirrai.Bayanan metagenomic sun nuna cewa kashi 91.4% na kwayoyin juriya na ƙwayoyin cuta yawanci ana gano su a duk sassan ƙasa huɗu da aka ambata a sama, waɗanda ke nuna salon ci gaba.16S rRNA amplicon sequencing ya haifar da jeri 29,285, wakiltar nau'ikan 346.Haɗuwa da nau'in sifofi na nau'in ta hanyar amplicon sequencing, an gano wannan watsawar ta kasance mai zaman kanta daga tushen microbiota mai alaƙa, duk da haka, ana iya sauƙaƙe ta hanyar wayar salula na kwayoyin halitta.Wannan binciken ya gano kwararar ARGs da ƙwayoyin cuta daga ƙasa zuwa cikin tsire-tsire ta hanyar ci gaba da tushen ƙasa mai alaƙa.

    Magana

    Wang, C., Hu, R., Mai ƙarfi, PJ, Zhuang, W., & Shu, L..(2020).Yawaitar kwayoyin juriya na ƙwayoyin cuta da ƙwayoyin cuta tare da tushen ƙasa-mangrove ci gaba.Jaridar Materials masu haɗari, 408, 124985.

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