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Tsarin Metagenomic -NGS

Metagenome yana nufin tarin tarin kwayoyin halitta na gauraya al'umma, kamar muhalli metagenome, mutum metagenome, da dai sauransu. Yana dauke da kwayoyin halittun da ake nomawa da wadanda ba za a iya nomawa ba.Tsarin metagenomic kayan aiki ne na kwayoyin halitta da ake amfani da shi don nazarin gaurayen kayan halitta da aka samo daga samfuran muhalli, wanda ke ba da cikakkun bayanai game da bambancin nau'in da yawa, tsarin yawan jama'a, dangantakar phylogenetic, kwayoyin aiki da hanyar sadarwa tare da abubuwan muhalli.

Dandalin:Illumina NovaSeq Platform


Cikakkun Sabis

Sakamakon Demo

Nazarin Harka

Amfanin Sabis

● Keɓancewa da noma-kyau don ƙayyadaddun bayanan ƙananan ƙwayoyin cuta

● Babban ƙuduri a cikin gano ƙananan nau'in nau'in nau'in nau'in nau'in nau'i a cikin samfurori na muhalli

● Ma'anar "meta-" ta haɗa dukkan siffofi na halitta a matakin aiki, matakin jinsin da kuma matakin jinsin halitta, wanda ke nuna ra'ayi mai ƙarfi wanda ya fi kusa da gaskiya.

● BMK yana tara ƙwarewa mai yawa a cikin nau'ikan samfuri daban-daban tare da samfuran sama da 10,000 da aka sarrafa.

Ƙayyadaddun Sabis

 Dandalin

Jeri

Bayanan da aka ba da shawarar

Lokacin juyawa

Illumina NovaSeq Platform

PE150

6 G/10 G/20 G

45 Kwanakin aiki

Binciken bioinformatics

● Raw data ingancin kula

● Metagenome taro

● Saitin kwayoyin halitta da ba a sake sakewa ba

● Nazari iri-iri

● Binciken bambancin aikin kwayoyin halitta

● Bincike tsakanin ƙungiyoyi

● Binciken ƙungiyar akan abubuwan gwaji

liuchengtu11

Samfuran Bukatun da Bayarwa

Samfuran Bukatun:

DominCire DNA:

Nau'in Misali

Adadin

Hankali

Tsafta

Cire DNA

ku 30ng

1 ng/l

OD260/280= 1.6-2.5

Don samfuran muhalli:

Nau'in samfurin

Hanyar samfurin da aka ba da shawarar

Ƙasa

Adadin samfur: kimanin.5 g;ku.Ya kamata a cire ragowar abin da ya bushe daga saman;Niƙa manyan guda kuma ku wuce ta 2 mm tace;Samfuran Aliquot a cikin bakararre EP-tube ko cyrotube don ajiya.

Najasa

Adadin samfur: kimanin.5 g;ku.Tattara da liquot samfurori a cikin bakararre EP-tube ko cryotube don ajiyar wuri.

Abubuwan ciki na hanji

Ana buƙatar sarrafa samfuran ƙarƙashin yanayin aseptic.Wanke nama da aka tattara tare da PBS;Centrifuge PBS kuma tattara hazo a cikin EP-tuben.

Lalacewa

Adadin samfur: kimanin.5 g;ku.Tattara da liquot samfurin sludge a cikin bakararre EP-tube ko cryotube don ajiya

Ruwan ruwa

Don samfurin tare da iyakataccen adadin ƙwayoyin cuta, kamar ruwan famfo, ruwan rijiya, da sauransu, Tattara ruwa aƙalla 1 L kuma ku wuce ta 0.22 μm tace don wadatar da ƙananan ƙwayoyin cuta akan membrane.Ajiye membrane a cikin bututu bakararre.

Fatar jiki

A hankali a goge saman fata tare da swab ɗin auduga mai bakararre ko ɓawon tiyata sannan a sanya shi cikin bututu mara kyau.

Isar da Samfurin Nasiha

Daskare samfuran a cikin ruwa nitrogen na sa'o'i 3-4 kuma adana a cikin ruwa nitrogen ko -80 digiri zuwa ajiyar dogon lokaci.Ana buƙatar jigilar samfuri tare da bushe-kankara.

Gudun Aikin Sabis

samfurin bayarwa

Samfurin bayarwa

Shirye-shiryen Laburare

Gina ɗakin karatu

Jeri

Jeri

Binciken bayanai

Binciken bayanai

Bayan Sabis na siyarwa

Bayan-sayar da sabis


  • Na baya:
  • Na gaba:

  • 1.Histogram: Rarraba nau'ikan

    3

    2.Ayyukan kwayoyin halitta annotate zuwa KEGG hanyoyin rayuwa

    4

    3.Heat taswira: Ayyuka daban-daban dangane da yawan jinsin dangi54.Circos na CARD kwayoyin juriya

    6

    BMK Case

    Yawaitar kwayoyin juriya na ƙwayoyin cuta da ƙwayoyin cuta tare da ci gaba da tushen ƙasa-mangrove

    Buga:Jaridar Materials masu haɗari, 2021

    Dabarun tsarawa:

    Materials: DNA tsantsa guda hudu na tushen mangrove samfurori masu alaƙa: ƙasa marar shuka, rhizosphere, episphere da sassan endosphere
    Platform: Illumina HiSeq 2500
    Manufa: Metagenome
    16S rRNA gene V3-V4 yankin

    Sakamako mai mahimmanci

    Matsakaicin metagenomic da bayanan metabarcoding akan tushen ƙasa na ci gaba da tsiron mangrove an sarrafa su don yin nazarin yaduwar ƙwayoyin ƙwayoyin cuta na rigakafi (ARGs) daga ƙasa zuwa tsirrai.Bayanai na metagenomic sun nuna cewa kashi 91.4% na kwayoyin juriya na ƙwayoyin cuta an gano su a cikin dukkan sassan ƙasa huɗu da aka ambata a sama, waɗanda ke nuna yanayin ci gaba.16S rRNA amplicon sequencing ya haifar da jeri 29,285, wakiltar nau'ikan 346.Haɗuwa da nau'ikan bayanin nau'ikan ta hanyar tsarin amplicon, an gano wannan watsawar ta kasance mai zaman kanta daga tushen microbiota mai alaƙa, duk da haka, ana iya sauƙaƙe ta ta wayar hannu na abubuwan halitta.Wannan binciken ya gano kwararar ARGs da ƙwayoyin cuta daga ƙasa zuwa cikin tsire-tsire ta hanyar ci gaba da tushen ƙasa mai alaƙa.

    Magana

    Wang, C., Hu, R., Mai ƙarfi, PJ, Zhuang, W., & Shu, L..(2020).Yawaitar kwayoyin juriya na ƙwayoyin cuta da ƙwayoyin cuta tare da ƙasa-mangrove tushen ci gaba.Jaridar Materials masu haɗari, 408, 124985.

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