Tsarin Metagenomic -NGS
Metagenome tarin kayan kwayoyin halitta ne na al'umma mai gauraya ta halittu, kamar su metagenomes na muhalli da na ɗan adam. Ya ƙunshi kwayoyin halitta na ƙwayoyin cuta masu noma da waɗanda ba za a iya nomawa ba. Jerin kwayoyin halitta na Shotgun tare da NGS yana ba da damar yin nazarin waɗannan yanayin halittu masu rikitarwa da aka saka a cikin samfuran muhalli ta hanyar samar da fiye da bayanin tsarin halitta, yana ba da haske mai zurfi game da bambancin nau'ikan halittu, yanayin yalwa, da tsarin yawan jama'a masu rikitarwa. Bayan nazarin tsarin halitta, metagenomes na shotgun kuma yana ba da hangen nesa na aikin genomics, yana ba da damar bincika kwayoyin halitta da aka ɓoye da kuma rawar da suke takawa a cikin tsarin muhalli. A ƙarshe, kafa hanyoyin sadarwa tsakanin abubuwan kwayoyin halitta da abubuwan muhalli yana ba da gudummawa ga fahimtar cikakken hulɗar da ke tsakanin al'ummomin ƙwayoyin cuta da asalin muhallinsu. A ƙarshe, jerin kwayoyin halitta yana tsaye a matsayin kayan aiki mai mahimmanci don warware matsalolin kwayoyin halitta na al'ummomin ƙwayoyin cuta daban-daban, yana haskaka alaƙar da ke tsakanin kwayoyin halitta da muhalli a cikin waɗannan yanayin halittu masu rikitarwa.
Dandamali: Illumina NovaSeq da DNBSEQ-T7
Fa'idodin Sabis
●Warewa da Hanyar da Ba ta da Noma don Bayyanar Al'umma ta Ƙananan Kwayoyin cuta: Yana ba da damar tsara jerin abubuwan kwayoyin halitta daga halittu marasa noma.
●Babban Haske: Gano nau'ikan halittu masu ƙarancin yawa a cikin samfuran muhalli.
●Cikakken Nazarin Bayanan Halitta:Ba wai kawai an mayar da hankali kan bambancin tsarin haraji ba, har ma da bambancin aiki na al'umma.
●Kwarewa Mai Zurfi:Tare da nasarar rufe ayyukan metagenomics da yawa a fannoni daban-daban na bincike da kuma sarrafa sama da samfura 200,000, ƙungiyarmu tana kawo ɗimbin gogewa ga kowane aiki.
Bayanin Sabis
| Dandalin jerin abubuwa | Tsarin Jerin Abubuwa | Bayanan da aka ba da shawarar | Kula da inganci |
| Illumina NovaSeq ko DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Bukatun Sabis
| Mayar da Hankali (ng/µL) | Jimlar adadi (ng) | Ƙara (µL) |
| ≥1 | ≥30 | ≥20 |
● Ƙasa/laka: 2-3g
● Abubuwan da ke cikin hanji-dabba: 0.5-2g
● Abubuwan da ke cikin hanji - kwari: 0.1-0.25g
● Saman shuka (laka mai wadataccen abu): 0.5-1g
● Ruwan da aka ƙara wa ruwa mai laushi): 0.2-0.5g
● Najasa (manyan dabbobi): 0.5-2g
● Najasa (linzami): Hatsi 3-5
● Ruwan wankewar alveolar na huhu: takardar tacewa
● Taɓawa a farji: Taɓawa 5-6
● Taɓa fata/aljihu/shafawa/taɓawa ta baki mai laushi/shafawa ta makogwaro/shafawa ta dubura: swab 2-3
● Kwayoyin halitta na saman: swabs 5-6
● Jikin ruwa/iska/biofilm: takardar tacewa
● Endophytes: 2-3g
● Tabon Hakori: 0.5-1g
Tsarin Aikin Sabis
Isarwa samfurin
Gina ɗakin karatu
Jerin abubuwa
Nazarin bayanai
Ayyukan bayan-sayarwa
Ya haɗa da wannan bincike:
● Tsarin sarrafa ingancin bayanai
● Haɗuwar Metagenome da hasashen kwayoyin halitta
● Bayanin kwayoyin halitta
● Binciken bambancin alpha na taxonomic
● Binciken aiki na al'umma: aikin halittu, metabolism, juriya ga maganin rigakafi
● Bincike kan bambancin aiki da na tsarin haraji:
Binciken bambancin Beta
Binciken tsakanin ƙungiyoyi
Binciken alaƙa: tsakanin abubuwan muhalli da tsarin OUT da bambancin
Binciken Aiki: Juriyar CARD maganin rigakafi
Bambancin bincike na hanyoyin rayuwa na KEGG: taswirar zafi na manyan hanyoyi
Bambancin rarrabuwar taksi na Alpha: ma'aunin ACE
Bambancin rarrabawar nau'ikan nau'ikan nau'ikan nau'ikan beta: PCoA
Binciki ci gaban da ayyukan tsara metagenome na BMKGene suka samar tare da Illumina ta hanyar tarin wallafe-wallafe da aka tsara.
Hai, Q. da sauransu (2023) 'Binciken metagenomic da metabolism na canje-canje a cikin abubuwan da ke cikin hanji na kifin rainbow (Oncorhynchus mykiss) wanda ya kamu da cutar necrosis mai yaɗuwa a yanayin zafi daban-daban na ruwa',Iyakoki a Kimiyyar Kwayoyin Halitta, 14, shafi na 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. da sauransu (2023) 'Al'ummomin ƙananan halittu, kwayoyin halittar juriya, da haɗarin juriya a cikin tafkunan birane na yanayi daban-daban na trophic: Haɗin ciki da tasirin waje',Mujallar Ci gaban Kayayyaki Masu Haɗari,9, ku. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Binciken Metagenomic Ya Bayyana Bambancin Tsarin Halitta da Aiki Tsakanin Ƙananan Kwayoyin Halitta Masu Alaƙa da Ruwa da kuma Masu Tauri na Tumaki',Iyakoki a Kimiyyar Kwayoyin Halitta, 13, shafi na 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'Microbiota daga alade mai kiba Ningxiang yana sake haɗa metabolism na carnitine don haɓaka ajiyar kitse mai kitse a cikin aladu masu laushi na DLY',Ƙirƙirar, 4(5), shafi. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. da sauransu (2023) 'Bayanin metagenomic game da yuwuwar haɗarin da ke tattare da tarkacen filastik masu rai/marasa lalacewa da waɗanda ba filastik ba a saman da ƙasan Haihe Estuary, China',Kimiyyar Muhalli Gabaɗaya, 887, shafi. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
