Tsarin Metagenomic -NGS
Metagenome tarin jimillar kwayoyin halitta na gaurayawan al'umman halittu, kamar muhalli da metagenomes na mutum. Ya ƙunshi kwayoyin halitta na ƙwayoyin cuta da ake iya nomawa da waɗanda ba za a iya nomawa ba. Tsarin metagenomic na Shotgun tare da NGS yana ba da damar yin nazarin waɗannan ƙayyadaddun yanayin yanayin yanayin halitta wanda aka haɗa cikin samfuran muhalli ta hanyar samar da fiye da bayanan haraji, yana ba da ƙarin haske game da bambance-bambancen nau'in, haɓakar haɓaka, da hadaddun tsarin yawan jama'a. Bayan nazarin haraji, metagenomics na harbin bindiga kuma yana ba da hangen nesa mai aiki na genomics, yana ba da damar bincikar ƙwayoyin halittar da aka sanya su da rawar da suke takawa a cikin tsarin muhalli. A ƙarshe, kafa hanyoyin sadarwa tsakanin abubuwan halitta da abubuwan muhalli suna ba da gudummawa ga cikakkiyar fahimtar ma'amala tsakanin ƙananan ƙwayoyin cuta da asalinsu. A ƙarshe, jeri na metagenomic yana tsaye a matsayin kayan aiki mai mahimmanci don buɗe ɓoyayyiyar ɓarna na al'ummomi daban-daban na ƙananan ƙwayoyin cuta, yana haskaka alaƙa mai ban sha'awa tsakanin jinsin halittu da ilimin halittu a cikin waɗannan sarƙaƙƙiyar yanayin halittu.
Platform: Illumina NovaSeq da DNBSEQ-T7
Amfanin Sabis
●Keɓewa da Hanyar Noma don Ƙirƙirar Bayanan Ƙira ta Al'umma: Ba da damar jerin abubuwan kwayoyin halitta daga kwayoyin da ba za a iya nomawa ba.
●Babban ƙuduri: Gano ƙananan nau'in nau'i mai yawa a cikin samfuran muhalli.
●Cikakken Binciken Bioinformatics:An mai da hankali ba kawai ga bambancin haraji ba amma har ma da bambancin ayyuka na al'umma.
●Kyawawan Kwarewa:Tare da rikodin waƙa na nasarar rufe ayyukan metagenomics da yawa a cikin fannonin bincike daban-daban da sarrafa samfuran sama da 200,000, ƙungiyarmu tana kawo ƙwarewar ƙwarewa ga kowane aiki.
Ƙayyadaddun Sabis
| Dandalin jerin abubuwa | Dabarun Jeri | An ba da shawarar bayanai | Kula da inganci |
| Illumina NovaSeq ko DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Bukatun Sabis
| Hankali (ng/µL) | Jimlar adadin (ng) | girma (µL) |
| ≥1 | ≥30 | ≥20 |
● Ƙasa / sludge: 2-3g
● Abun ciki na ciki-dabba: 0.5-2g
● Abubuwan ciki na ciki-kwari: 0.1-0.25g
● Filayen shuka (labaran da aka wadatar): 0.5-1g
● Fermentation broth wadatar labe: 0.2-0.5g
● Faeces (manyan dabbobi): 0.5-2g
● Faeces ( linzamin kwamfuta): 3-5 hatsi
● Ruwan lavage na alveolar na huhu: takarda tace
● Swab na Farji: 5-6 swabs
● Fatar fata / al'aura swab / miya / nama mai laushi na baki / pharyngeal swab / swab swab: 2-3 swabs
● Ƙananan ƙwayoyin cuta: 5-6 swabs
● Ruwa / iska / biofilm: takarda tace
● Endophytes: 2-3g
● Plaque na hakori: 0.5-1g
Gudun Aikin Sabis
Samfurin bayarwa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Ya haɗa da bincike mai zuwa:
● Tsarin sarrafa ingancin bayanai
● Taron metagenome da tsinkayar kwayoyin halitta
● Bayanan Halittu
● Taxonomic alpha diversity analysis
● Nazarin aiki na al'umma: aikin nazarin halittu, rayuwa, juriya na rigakafi
● Nazari akan bambancin aiki da na haraji:
Binciken bambancin beta
Binciken Inter-group
Binciken daidaitawa: tsakanin abubuwan muhalli da abubuwan OUT da bambancin
Binciken aiki: juriya na ƙwayoyin cuta na CARD
Binciken bambancin hanyoyin hanyoyin rayuwa na KEGG: taswirar zafi na mahimman hanyoyin
Bambancin Alpha na rarraba haraji: ACE index
Bambancin Beta na rarraba haraji: PCoA
Bincika ci gaban da BMKGene's metagenome sequencing sabis tare da Illumina ya sauƙaƙe ta hanyar tarin wallafe-wallafe.
Hai, Q. et al. (2023) 'Binciken Metagenomic da metabolomic na canje-canje a cikin abun ciki na hanji na bakan gizo na bakan gizo (Oncorhynchus mykiss) wanda ya kamu da kwayar cutar hematopoietic necrosis a yanayin yanayin ruwa daban-daban',Frontiers a Microbiology, 14, p. 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. et al. (2023) 'Al'ummomin microbial, kwayoyin juriya, da kuma kasada a cikin tafkunan birane na jihohin trophic daban-daban: Hanyoyin ciki da tasirin waje',Jaridar Ci gaban Abubuwan Abubuwan Haɗaɗɗen Ci Gaba,9, ku. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Binciken Metagenomic Ya Bayyana Bambance-Bambance a cikin Haɗawa da Aiki Tsakanin Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru na Rumen Tumaki',Frontiers a Microbiology, 13, p. 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'Microbiota mai kiba Ningxiang alade ya sake dawo da metabolism na carnitine don haɓaka jijiya mai kitse mai tsoka a cikin aladun DLY mai raɗaɗi',Innovation, 4(5), shafi. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Bayyanawar metagenomic game da yuwuwar haɗarin wakili na halitta / filastik da ba za a iya lalacewa ba da tarkacen filastik a sama da ƙasa na Haihe Estuary, China',Kimiyya na Jimillar Muhalli, 887, shafi. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
