mRNA-seq (NGS) - Sabon zamani

Tsarin mRNA yana ba da damar yin bayanin dukkan bayanan mRNA a cikin ƙwayoyin halitta a ƙarƙashin takamaiman yanayi kuma fasaha ce da ake amfani da ita sosai a fannoni daban-daban na bincike. An tsara bututun mRNA-seq na BMKCloud De novo don yin nazarin ɗakunan karatu masu wadatar poly-A lokacin da babu wani nau'in halittar da aka ambata. Tsarin bututun yana farawa da sarrafa inganci, sannan kuma ya biyo baya.na sabuwar shekaraTarin bayanai da zaɓin saitin unigene. Binciken tsarin unigene yana annabta jerin lambobi (CDS) da maimaita jerin abubuwa masu sauƙi (SSR). Daga baya, nazarin bayyanar bambance-bambancen yana gano kwayoyin halitta da aka bayyana daban-daban (DEGs) tsakanin yanayin da aka gwada, sannan sai bayanin aiki da wadatar DEGs don fitar da fahimtar halittu.