Nanopore cikakken rubutu mai tsayi

Tsarin rubutun Nanopore hanya ce mai ƙarfi don tsara cikakken cDNAs, gano da kuma ƙididdige isoforms na rubutun daidai. An tsara bututun rubutun BMKCloud Nanopore Full-Length Transcriptionline don nazarin bayanan RNA-Seq da aka samar akan dandamalin Nanopore akan wani kwayar halitta mai inganci mai kyau, yana samar da bincike mai inganci da adadi a duka kwayar halitta da matakin rubutun. Bayan sarrafa inganci, ana samun jerin cikakkun marasa chimeric (FLNC) kuma ana tsara jerin yarjejeniya zuwa ga kwayar halittar tunani don cire takaddun da ba a cika ba. Daga wannan saitin rubutun, ana ƙididdige magana kuma ana gano kwayoyin halitta daban-daban kuma ana tantance bayanan aikin. Tsarin ya kuma haɗa da nazarin polyadenylation (APA), nazarin haɗin gwiwa na madadin, nazarin maimaita jerin sauƙaƙa (SSR), hasashen lncRNA da maƙasudai masu dacewa, hasashen jerin lambobi (CDS), nazarin dangin kwayoyin halitta, nazarin abubuwan rubutun, hasashen sabbin kwayoyin halitta da bayanin aiki na takardun.