Ƙaramin Jerin RNA-Illumina
Siffofi
● Shirye-shiryen ɗakin karatu na zaɓin girma.
● Binciken halittu masu rai ya mayar da hankali ne kan hasashen miRNA da kuma abubuwan da ake nufi.
Fa'idodin Sabis
●Ƙwarewa Mai ZurfiMun sarrafa sama da samfura 300, waɗanda suka ƙunshi nau'ikan samfura daban-daban. Muna kawo wadatar ƙwarewa ga kowane aiki.
●Sarrafa Inganci Mai Tsauri: Muna aiwatar da mahimman wuraren sarrafawa a duk matakai, tun daga shirye-shiryen samfura zuwa shirye-shiryen ɗakin karatu, jerin abubuwa da kuma ilimin halittu. Sa ido mai kyau yana tabbatar da isar da sakamako mai inganci akai-akai.
●Cikakken nazarin bioinformatics:Yana ba da damar gano miRNAs da aka sani da kuma waɗanda aka saba gani, gano maƙasudin miRNAs, da kuma bayanin aiki da haɓaka su tare da bayanai da yawa (KEGG, GO).
●Tallafin Bayan Tallace-tallace: Mun fahimci muhimmancin kasancewa a wurin, shi ya sa alƙawarinmu ya wuce kammala aikin tare da tsawon watanni 3 bayan an sayar da shi. A wannan lokacin, muna ba da shawarwari kan aikin, taimakon magance matsaloli, da kuma zaman tambayoyi da amsoshi don magance duk wata tambaya da ta shafi sakamakon.
Samfurin Bukatun da Isarwa
| Laburare | Dandalin | Bayanan da aka ba da shawarar | Takaddun bayanai (QC) |
| An zaɓi girman | Illumina SE50 | Karatu na 10M-20M | Q30≥85% |
Bukatun Samfura:
Nukliyoyin Nukliyoyin:
| Conc.(ng/μl) | Adadi (μg) | Tsarkaka | Mutunci |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 An nuna ƙarancin gurɓataccen furotin ko DNA a kan gel ɗin. | RIN≥6.0; 5.0≥28S/18S≥1.0; iyaka ko babu matakin tushe |
● Shuke-shuke:
Tushe, Tushe ko Furen Fure: 450 MG
Ganye ko Iri: 300 MG
'Ya'yan itace: 1.2 g
● Dabbobi:
Zuciya ko Hanji: 450 MG
Jijiyoyin ciki ko kwakwalwa: 240 MG
Tsoka: 600 MG
Kashi, Gashi ko Fata: 1.5g
● Arthropods:
Kwari: 9g
Crustacea: 450 MG
● Jini gaba ɗaya: Bututu 2
● Kwayoyin halitta: 106 ƙwayoyin halitta
● Magani da Jini:6 mL
Isarwa Samfurin da Aka Ba da Shawara
Akwati: Bututun centrifuge na 2 ml (Ba a ba da shawarar a saka tin foil ba)
Lakabi samfurin: Rukuni+kwafi misali A1, A2, A3; B1, B2, B3.
Jigilar kaya:
1. Busasshen Kankara: Ana buƙatar a saka samfuran a cikin jakunkuna a binne su a cikin busasshen kankara.
2. Bututun RNA masu karko: Ana iya busar da samfuran RNA a cikin bututun daidaita RNA (misali RNAstable®) kuma a aika su a zafin ɗaki.
Tsarin Aikin Sabis
Tsarin gwaji
Isarwa samfurin
Fitar da RNA
Gina ɗakin karatu
Jerin abubuwa
Nazarin bayanai
Ayyukan bayan-sayarwa
Bioinformatics
● Kula da ingancin bayanai na ɗan gajeren lokaci
● Ƙaramin rarrabuwar RNA
● nazarin bayyanar miRNA da bambancin bayyanar
● kwayar halittar da aka yi niyya ta miRNA da kuma bayanin kwayar halittar da aka yi niyya ta miRNA da aka bayyana daban-daban
● Bayanin kwayar halitta da aka yi niyya da kuma haɓaka aikin kwayar halittar miRNA da aka bayyana daban-daban
Gano miRNA: tsari da zurfin
Bambancin bayyanar miRNA - tarin tsari
Bayanin aiki na maƙasudin miRNAs da aka bayyana daban-daban
Binciki ci gaban bincike da ayyukan tsara sRNA na BMKGene suka taimaka ta hanyar tarin wallafe-wallafe da aka tsara.
Chen, H. da sauransu (2023) 'Kamuwa da cuta yana hana samar da sinadarin saponin da photosynthesis a cikin Panax notoginseng',Ilimin Halittar Shuke-shuke da Kimiyyar Halitta, 203, shafi na 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. da sauransu (2023) 'Shuka FYVE mai dauke da furotin FREE1 yana hadewa da sassan microprocessor don danne kwayar halittar miRNA',Rahotannin EMBO, 24 (1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. da sauransu (2023) 'MicroRNA Ame-Bantam-3p Yana Sarrafa Ci gaban Tsutsa ta hanyar Niyya ga Yankunan 8 Gene (megf8) masu kama da Ci gaban Epidermal da yawa a cikin Kudan zuma, Apis mellifera',Mujallar Duniya ta Kimiyyar Molecular, 24(6), shafi na 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. da sauransu (2018) 'Binciken Haɗaɗɗen MiRNA da Kwayoyin Halitta da ke da Alaƙa da Ingancin Nama Ya Bayyana Cewa Gga-MiR-140-5p Yana Shafar Daskarewar Kitse a Cikin Jijiyoyin Kaji',Ilimin Halittar Jiki da Kimiyyar Halitta, 46 (6), shafi na 2421-2433. doi: 10.1159/000489649.
