Ƙananan RNA Sequencing-Illumina
Siffofin
● Girman zaɓi shirye-shiryen ɗakin karatu.
● Binciken bioinformatic ya ta'allaka ne akan hasashen miRNA da makasudi.
Amfanin Sabis
●Ƙwararren Ƙwararru: Mun sarrafa samfurori sama da 300, wanda ke ɗaukar nau'ikan samfuri daban-daban. Muna kawo arziƙin gwaninta ga kowane aiki.
●Tsananin Kula da Inganci: Muna aiwatar da mahimman abubuwan sarrafawa a duk matakai, daga shirye-shiryen samfurin zuwa shirye-shiryen ɗakin karatu, jerin abubuwa da bioinformatics. Sabis ɗinmu mai kyau yana tabbatar da isar da sakamako mai inganci akai-akai.
●Cikakken nazarin bioinformatics:Yana ba da damar gano miRNAs da aka sani da na zamani, gano maƙasudin miRNAs, da madaidaicin bayanin aiki da haɓakawa tare da bayanai masu yawa (KEGG, GO).
●Tallafin Bayan Talla: Mun fahimci mahimmancin kasancewar kasancewa, shine dalilin da ya sa sadaukarwarmu ta wuce bayan kammala aikin tare da lokacin sabis na watanni 3 bayan siyarwa. A wannan lokacin, muna ba da bibiyar aikin, taimako na warware matsala, da kuma zaman Q&A don magance duk wata tambaya da ta shafi sakamakon.
Samfuran Bukatun da Bayarwa
| Laburare | Dandalin | Bayanan da aka ba da shawarar | Bayanan Bayani na QC |
| Girman da aka zaɓa | Illumina SE50 | 10M-20M ya karanta | Q30≥85% |
Samfuran Bukatun:
Nucleotides:
| Conc.(ng/μl) | Adadin (μg) | Tsafta | Mutunci |
| ≥ 80 | 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Iyakance ko babu furotin ko gurɓataccen DNA da aka nuna akan gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; iyakance ko babu girma daga tushe |
● Shuke-shuke:
Tushen, kara ko petal: 450 MG
Leaf ko iri: 300 MG
'Ya'yan itace: 1.2 g
● Dabbobi:
Zuciya ko hanji: 450 MG
Viscera ko Brain: 240 MG
tsoka: 600 MG
Kasusuwa, Gashi ko Fata: 1.5g
● Arthropods:
Kwari: 9g
Crustacea: 450 MG
● Jini duka: 2 bututu
● Kwayoyin: 106 Kwayoyin
● Magani da Plasma:6 ml
Isar da Samfurin Nasiha
Kwantena: 2 ml bututu centrifuge (Ba a ba da shawarar foil tin ba)
Alamar samfur: Ƙungiya+ kwafi misali A1, A2, A3; B1, B2, B3.
Jirgin ruwa:
1. Busasshen ƙanƙara: Ana buƙatar samfurori a cikin jaka kuma a binne su a bushe-kankara.
2. RNAstable tubes: Ana iya bushe samfuran RNA a cikin bututun daidaitawar RNA (misali RNAstable®) kuma a tura su cikin zafin jiki.
Gudun Aikin Sabis
Gwajin ƙira
Samfurin bayarwa
RNA cirewa
Gina ɗakin karatu
Jeri
Binciken bayanai
Bayan-sayar da sabis
Bioinformatics
● Raw data ingancin kula
● Ƙananan rarraba RNA
● Maganar miRNA da nazarin magana daban
● Halin da aka yi niyya na miRNA da mabambantan bayanin miRNA manufa ta asali
● Ƙididdigar ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun kwayoyin halittar miRNA da aka bayyana daban
Gano miRNA: tsari da zurfin
Bambance-bambancen magana na miRNA - tari na matsayi
Bayanin aiki na manufa na miRNAs daban-daban da aka bayyana
Bincika ci gaban binciken da BMKGene'sRNA sabis na jerin ayyuka ya sauƙaƙe ta hanyar tarin wallafe-wallafe.
Chen, H. et al. (2023) 'Cutar kamuwa da cuta ta hana saponin biosynthesis da photosynthesis a cikin Panax notoginseng',Physiology Plant da Biochemistry, 203, p. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) 'Tsarin FYVE yanki mai ɗauke da furotin FREE1 yana haɗe da abubuwan microprocessor don murkushe biogenesis na miRNA',Rahoton EMBO, 24 (1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yau, J. et al. (2023) 'MicroRNA Ame-Bantam-3p Yana Sarrafa Ci gaban Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararrun Ƙwararru-kamar Domains 8 Gene (megf8) a cikin Honeybee, Apis mellifera',Jaridar Duniya ta Kimiyyar Kwayoyin Halitta, 24(6), shafi. 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Hanƙan Bincike na MiRNA da Genes Haɗe da Ingantacciyar Nama Ya Nuna cewa Gga-MiR-140-5p Yana Shafar Fat Intramuscular Deposition A cikin Kaji',Ilimin Halittar Halitta da Halitta, 46 (6), shafi na 2421-2433. doi: 10.1159/000489649.
