Gabaɗayan jerin abubuwan genome bisulfite
Amfanin Sabis
● Cikakken dandamali: samar da kyakkyawan sabis na tsayawa ɗaya daga sarrafa samfurin, ginin ɗakin karatu, jeri zuwa nazarin bioinformatics.
● Babban daidaito: Balagagge methylation fasahar sarrafa canji na iya yin daidai daidai da matsayin methylation na tushen C guda ɗaya.
● Faɗin ɗaukar hoto: gano wuraren methylation a matakin genome-fadi.
● Kyakkyawan maimaitawa: barga aiki, dace da kwatanta kwatanta tsakanin samfurori masu yawa.
Ƙayyadaddun Misali
|
Dandalin
|
Tsawon Karatu
|
Dabarun Jeri
|
| Illumina | Farashin PE150 | 30X |
Samfuran Bukatun
|
Nau'in Misali
|
Adadin (μg)
|
Conc.(ng/μl)
|
girma (μl)
|
Tsafta
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| Iyakance lalacewa da gurɓatawa
|
Gudun Aikin Sabis
Samfurin bayarwa
Cire DNA
Gina ɗakin karatu
Jeri
Binciken bayanai
Isar da bayanai
Bioinformatics
1.Gome-fadi DNA methylation rarraba
2.Annotation a kan sosai-methylated CGI yankin
samun zance
Ku rubuta sakonku anan ku aiko mana
