16s / 18s / kona amphalion serenencing-pacbio
Nā hiʻohiʻona lawelawe
● EXEQUNCING PRESTORMS: Pacbio redo
● SEQUENCING MODE: CCS (HiFi heluhelu)
●ʻO ka hōʻiliʻili o nā wahi i kauʻia e ka helu tandem e pili ana i nā amplicons ma mua o ka hoʻomākaukauʻana o ka hale kūʻai hale kūʻai
Loaʻa nā lawelawe lawelawe
●Hōʻailona'ordonoidIic Resolution: Tha han pokololi sququing,Hoʻonohoʻia nā helu helu helu Otubing Otute i nā pae kiʻekiʻe.
●Ke kāhea nuiʻana: Pacbio CCS mode SCS SEQUENCING (HUFI heluhelu).
●Hoʻokaʻawale-free:ʻO ka hōʻike wikiwikiʻana o ka microbal e pili ana i nā hiʻohiʻona o nā kaiāulu.
●Pili loa: Nā haʻawina kaiāuluʻo Duet Selebial.
●ʻO ka nānāʻana o Bioinia Companintisticictic:ʻO ka uku QIME2 pākuʻi (ʻike nui loa i loko o ka ecology microbial) me nā mea kiko'ī e pili ana i ka waihona pūnaewele, Otuv.
●ʻO kaʻike nui: Me nā kaukani o nā papahana amplicon seaquincing i hanaʻia, e lawe mai i ka bmkgene i kahi kūlana kiʻekiʻe, a me ke kākoʻo maikaʻi loa.
Nāʻikepili lawelawe
| Puka Hanau | Serenencing hoʻolālā | Ua'ōleloʻia nāʻikepili |
| Amplion | Redo curni | 10/30/50 K Tags (CCS) |
Nā koi koi
| Ka Manaʻo (NG / μl) | Ka nui nui (μg) | Nui (μl) |
| ≥5 | ≥0.3 | ≥20 |
Hōʻikeʻia ka hoʻounaʻana
E hoʻokaʻawale i nā'āpana i nā nitrogen wai no 3-4 mau hola a kūʻai aku i ka hale o ka wai wai lōʻihi. Hōʻikeʻia ka hoʻounaʻana me ka hau me ka hau maloʻo.
E holo ka lawelawe lawelawe
Nā Hoʻounaʻana
Hana ka waihona puke
Hoahewa ana
Nāʻikepiliʻikepili
Ma hope o nā lawelawe kūʻai
Komo i ka loiloi ma lalo nei:
● Ke Kahu Pūnaewele Pūnaewele
● OT OTU Clustering / de-wiy (asv)
● Otou Annotation
● Alpha waena o ka papa inoa o ka alpha diversity: nā helu helu he nui, me ka shannon,ʻo Simpson a me Ace.
● Beta Hālāwai Kahiko
● Hoʻonani-hui
● Hoʻohālikelikeʻikepili: ma waena o nā kumu kūlohelohe a me keʻano likeʻole
● 16s friend faile
Kānana o ka hoʻolahaʻana o kaʻauhauʻauhau
Kaʻike kaiāulu
Hōʻikeʻike Alphaversity: Ace
kuhikuhi
Beta i nā hiʻohiʻona hoʻohālikelike: pcoa
ʻO ka loiloi Intergroup: anvava
Eʻimi i ka holomua o nā mea holomua e BMKCONE's AMPLIND SEQILING PERSICE me ka pacbio ma o kahi hōʻiliʻili o nā paʻi.
Gao, X. a me Wang, H. 1), p. 16. Doi: 10.3390 / Firmentation9010016 / S1.
Li, s. et al. (2023) 'O ka hopuʻana i keʻano o ka microbial ma nā wahi a pau i ka hoʻohanaʻana i nā mea kanu lāʻau a me nā micriboms a me nā micj biofilcles 2023 9 Doi: 10.10338 / s41522-023-00439-8.
Mu, l. et al. (2022)ʻO nā hopena o nā momona momona momona ma nāʻano hana fermentation 1487. Doi: 10.1002 / Jsfa.11482.
Yang, j. et al. (2023) i loko o Oxazolne-indivad inika insidaned styestinalsed styestinalse string ma ka zebrafish ', antiomidants 2023, vol. 12,ʻaoʻao 192, 12 (1), p. 192. Doi: 10.3390 / Antiob12010192.
