ʻO nā hale waihona puke mua
Nā hiʻohiʻona
●Nā platform:'Ollumina novaseq 6000 a me Novaseq x Plus
●ʻO nā modes:Pe150 a me pe250
●ʻO ka mana kūpono o nā hale waihona puke ma mua o ka selencing
●Sequencing data QC a me ka laweʻana:ʻO ka hāʻawiʻana i ka hōʻike QC a me nāʻikepili i ke kiʻi wikiwiki ma hope o ka demultiplexing a me kānana
Loaʻa nā lawelawe lawelawe
●ʻO kaʻoiaʻiʻo o nā lawelawe sequence:Hiki i ka mea kūʻai ke koho i keʻano o ke ala, e kaheʻana o ka cell, a iʻole ka nui o nāʻikepili i koiʻia (mahele'āpana.
●ʻO kaʻike ākea ma Imumina seaquence Stacentform:Me nā kaukani o nā papahana i paniʻia me nāʻano likeʻole.
●KAHANA O KA LOHE ANA O NUI QC:Me nā metricits maikaʻi,ʻo kaʻikeʻikepili a me ka hana holoʻokoʻa o ka papahana seequence.
●HE KAI LOA NUI:me ka huli pōkole-a puni ka manawa.
●ʻO ka mana kūpono: Ua hoʻokō mākou i nā koi QC e hōʻoia i ka hōʻoiaʻana i ka hāʻawiʻana i nā hopena kiʻekiʻe.
Nā Planetpums
| Kahua | Kahe cell | ʻO keʻano serenencing | Kōho | Kuhiʻia |
| Nvaseq x | 10b (8 lanes) | Peemona | Mea kū hoʻokahi 'Āpanaʻaoʻao | 375GB / Lane |
| 25b (8 mau laina) | Peemona | Mea kū hoʻokahi 'Āpanaʻaoʻao | 1000 GB / Lane | |
| Novaseq 6000 | SP Wab Pepe (2 Lanes) | Pe250 | Kahe cell Mea kū hoʻokahi 'Āpanaʻaoʻao | 325-400 m heluhelu / Lane |
| S4 flow cell (4 lanes) | Peemona | Kahe cell Mea kū hoʻokahi 'Āpanaʻaoʻao | ~ 800 gb / ala |
Nā koi koi
| Ka helu helu (x) | Ka noʻonoʻo (qpcr / nm) | Volume | |
| Māhele Lane Senceence
| X ≤ 10 gb | ≥ 1 nm | ≥ 25 μl |
| 10 GB <x ≤ 50 GB | ≥ 2 nm | ≥ 25 μl | |
| 50 GB <x ≤ 100 GB | ≥ 3 nm | ≥ 25 μl | |
| X> 100 GB | ≥ 4 nm | ≥ 25 μl | |
| Lane Sequencing | Ma ke ala | ≥ 1.5 nm / ka waihona puke | ≥ 25 μl / hale waihona puke |
Ma kahi o ka loaʻaʻana a me ka nui o ka nui, e koiʻia kahiʻano kūpono kūpono.
Kahakaha: Lane Sequening o nā hale waihona liʻiliʻi e pono aiʻo PHIX Spike
Manaʻo mākou e waiho i nā hale waihona puke mua ma mua o nā hiʻohiʻona. Inā makemakeʻoe iā bmkgene e hana i ka hana o ka waihona puke, eʻoluʻolu e pili
ʻO nā koina waihona no ka māhele'āpana Lane.
Ka waihona waihona (Peak Map)
Ponoʻo Main Peak i loko o 300-450 BP.
Pono nā hale waihona puke i hoʻokahi pākuʻi nui,ʻaʻohe adapter e kū ana aʻaʻohe dimers mua.
Eʻoluʻolu e hōʻea iā mākou ināʻaʻole e hoʻokō kāu mau hiʻohiʻona i nā pono e hoʻomaka ana i nā pono.
Haʻawina lawelawe
Palapala inoa
Hoahewa ana
ʻO ka mana o nāʻikepili
Nā Hana Hana
Hōʻike QC waihona puke
Hāʻawiʻia kahi hōʻike ma keʻano o ka waihona puke ma mua o ka seleving, e nānā ana i ka nui o ka waihona kālā, a me ka hoʻopunipuni.
Seequing qc hōʻike
Papa 1. Nā helu helu ma nā helu sequencing.
| Kōkuhi ID | BMKID | Heluhelu mai nā ana | ʻO nāʻikepili RAW (bp) | E hoʻomaʻemaʻeʻia (%) | Q20 (%) | Q30 (%) | GC (%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | Bmk_02 | 14,717,,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Kiʻi 1.ʻO ka hoʻokaʻawaleʻana o ka maikaʻi ma ka heluheluʻana i kēlā me kēia hoʻohālike
Kiʻi 2. Kūʻai Kūlana Kūlana
Kiʻi 3.ʻO ka hāʻawiʻana i nā mea heluhelu heluhelu i nāʻikepili seequing
