Ka Nānā ʻAna o ka Hui Pūʻulu Genome
Kaʻina hana
Nā Pōmaikaʻi o ka Lawelawe
●Nā moʻolelo ʻike loea a me nā hoʻolaha nuiMe ka ʻike i hōʻiliʻili ʻia ma GWAS, ua hoʻopau ʻo BMKGene i nā haneli o nā papahana ʻano ma ka noiʻi GWAS heluna kanaka, ua kōkua i nā mea noiʻi e paʻi i nā ʻatikala he 100 a ʻoi, a ua hōʻea ka hopena hopena cumulative i 500.
● Ka loiloi bioinformatics piha: ʻo ke kaʻina hana e komo pū ana me ka nānā ʻana i ka pilina SNP-trait, e hāʻawi ana i kahi hoʻonohonoho o nā genes moho a me kā lākou hōʻike hana pili.
●ʻO ka hui bioinformatics akamai loa a me ke kaʻina hana loiloi pōkoleme ka ʻike nui i ka loiloi genomics holomua, hāʻawi ka hui o BMKGene i nā loiloi piha me ka manawa hoʻololi wikiwiki.
●Kākoʻo Ma hope o ke kūʻai aku:ʻOi aku kā mākou kūpaʻa ma mua o ka hoʻopau ʻana o ka papahana me kahi manawa lawelawe ma hope o ke kūʻai aku he 3 mahina. I loko o kēia manawa, hāʻawi mākou i ka hahai ʻana i ka papahana, ke kōkua hoʻoponopono pilikia, a me nā hālāwai Q&A e pane i nā nīnau e pili ana i nā hopena.
Nā kikoʻī lawelawe a me nā koi
| ʻAno o ke kaʻina hana | Ka nui o ka heluna kanaka i manaʻo ʻia | Hoʻolālā hoʻonohonoho | Nā koi nucleotide |
| Ka Hoʻonohonoho ʻana o ka Genome Holoʻokoʻa | 200 mau laʻana | 10x | Ka nui o ka wai: ≥ 1 ng/ µL Huina nui≥ 30ng Ka palena a ʻaʻohe paha o ka hōʻino a i ʻole ka haumia |
| ʻĀpana Hoʻonui ʻia o ka Locus Kūikawā (SLAF) | Ka hohonu o ka lepili: 10x Helu o nā lepili: < 400 Mb: Paipai ʻia ʻo WGS < 1Gb: 100K mau lepili 1Gb > 2Gb: 300K mau lepili Nā lepili 500k kiʻekiʻe loa | Ka nui o ka hui ʻana ≥ 5 ng/µL Huina nui ≥ 80 ng Nanodrop OD260/280=1.6-2.5 ʻO ka gel Agarose: ʻaʻohe a i ʻole ka palena o ka hoʻohaumia a i ʻole ka haumia
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Koho Mea Hana
Nā ʻano like ʻole, nā ʻano liʻiliʻi, nā ʻāina/nā waihona gene/nā ʻohana hui pū ʻia/nā kumuwaiwai hihiu
Nā ʻano like ʻole, nā ʻano liʻiliʻi, nā ʻāina
ʻOhana hapa hoahānau/ʻohana hoahānau piha/nā kumuwaiwai hihiu
Kahe Hana Lawelawe
Hoʻolālā hoʻokolohua
Hoʻouna ʻana i nā laʻana
Ka unuhi ʻana o ka RNA
Ke kūkulu ʻana i ka hale waihona puke
Ka hoʻonohonoho ʻana
Ka loiloi ʻikepili
Nā lawelawe ma hope o ke kūʻai aku
Aia pū kekahi loiloi ma lalo nei:
- Ka loiloi ʻana o ka hui ʻana o ka genome: LM, LMM, EMMAX, kumu hoʻohālike FASTLMM
- Ka hoʻākāka hana o nā genes moho
Ka loiloi ʻana o ka hui ʻana o ke ʻano SNP - Manhattan plot
Ka loiloi ʻana o ka pilina SNP-ʻano - QQ plot
E ʻimi i nā holomua i kōkua ʻia e nā lawelawe de GWAS a BMKGene ma o kahi hōʻiliʻili o nā puke i hoʻonohonoho pono ʻia:
Lv, L. et al. (2023) 'ʻIke i ke kumu genetic o ka hoʻomanawanui ammonia i loko o ka razor clam Sinonovacula constricta e ka genome-wide association study',Ka mahiʻai ʻana i ka wai, 569, ʻaoʻao 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
Li, X. et al. (2022) 'Hōʻike nā loiloi Multi-omics o 398 mau ʻāpana millet foxtail i nā ʻāpana genomic e pili ana i ka domestication, nā ʻano metabolite, a me nā hopena anti-inflammatory',Mea kanu Molekala, 15(8), pp. 1367–1383. doi: 10.1016/j.molp.2022.07.003.
Li, J. et al. (2022) 'Genome-Wide Association Mapping of Hulless Barely Phenotypes in Drought Environment',Nā Palena ma ka ʻEpekema Mea Kanu, 13, ʻaoʻao 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. et al. (2021) 'ʻO GmST1, ka mea e hoʻopili ana i kahi sulfotransferase, e hāʻawi ana i ke kū'ē ʻana i nā ʻano maʻi soybean mosaic G2 a me G3',Mea kanu, Kelepona a me ke Kaiapuni, 44(8), pp. 2777–2792. doi: 10.1111/PCE.14066.
