Long Non-coding Sequencing-Illumina
Pono lawelawe
●Hoʻopili hui o mRNA a me lncRNA: ma ka hoʻohui ʻana i ka helu ʻana o nā transcripts mRNA me ke aʻo ʻana i ka lncRNA a me kā lākou mau pahuhopu, hiki ke kiʻi i kahi ʻike hohonu o ke ʻano hoʻoponopono i lalo o ka pane kelepona.
●ʻIke Nui: Hāʻawi kā mākou hui i ka waiwai o ka ʻike i kēlā me kēia papahana, me kahi moʻolelo o ka hana ʻana ma luna o 23,000 mau laʻana ma BMK e pili ana i nā ʻano laʻana like ʻole a me nā papahana lncRNA.
●Hoʻomalu maikaʻi loa: Hoʻokō mākou i nā wahi mana koʻikoʻi ma nā pae āpau, mai ka laʻana a me ka hoʻomākaukau ʻana i ka waihona a hiki i ka sequencing a me ka bioinformatics. Mālama kēia mākaʻikaʻi ʻana i ka hāʻawi ʻana i nā hopena kiʻekiʻe mau.
●Kākoʻo ma hope o ke kūʻai aku: ʻO kā mākou kūpaʻa e ʻoi aku ma mua o ka hoʻopau ʻana o ka papahana me kahi manawa lawelawe 3 mahina ma hope o ke kūʻai aku. I kēia manawa, hāʻawi mākou i ka hahai ʻana o ka papahana, kōkua hoʻoponopono, a me nā hālāwai Q&A e hoʻoponopono i nā nīnau pili i nā hopena.
Nā Koina Laʻana a me ka Hoʻouna ʻana
| Hale Waihona Puke | Papahana | Manaʻo ʻikepili | ʻIkepili QC |
| Ua pau ka waihona kuhikuhi rRNA | Illumina PE150 | 10-16 Gb | Q30≥85% |
Nucleotides:
| Conc.(ng/μl) | Ka nui (μg) | Maemae | Pono pono |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Hoʻopaʻa ʻia a ʻaʻole hoʻi i ka protein a i ʻole DNA contamination i hōʻike ʻia ma ka gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; palena a ʻaʻole kiʻekiʻe kumu |
● Nā mea kanu:
Aʻa, ʻaʻa a i ʻole Petal: 450 mg
Lau a Hua paha: 300 mg
Hua: 1.2 g
● holoholona:
Naʻau a i ʻole ka ʻōpū: 450 mg
ʻO Viscera a i ʻole lolo: 240 mg
ʻiʻo: 600 mg
Iwi, lauoho a ʻili paha: 1.5g
● Nā ʻāʻī:
Nā Insect: 9g
Crustacea: 450 mg
● Ke koko holoʻokoʻa:2 paipu
● Nā pūnaewele: 106 pūnaewele
● Serum a me ka Plasma: 6 mL
Manaʻo ʻia ka hāʻawi laʻana
Pahu: 2 ml centrifuge tube (ʻAʻole ʻōlelo ʻia ʻo Tin foil)
Laʻana lepili: Pūʻulu+hoʻopiʻi e laʻa me A1, A2, A3; B1, B2, B3.
Hoʻouna:
1. Ka hau maloo: Pono e hoʻopili ʻia nā laʻana i loko o nā ʻeke a kanu ʻia i loko o ka hau maloʻo.
2. Nā paipu RNAstable: Hiki ke hoʻomaloʻo ʻia nā laʻana RNA i loko o ka paipu hoʻopaʻa RNA (e laʻa me RNAstable®) a hoʻouna ʻia i loko o ka lumi wela.
Holo Hana Hana
Hoʻolālā hoʻokolohua
Hāʻawi laʻana
RNA unuhi
Kūkulu hale waihona puke
Ke kaʻina ʻana
ʻIkepili ʻikepili
Nā lawelawe ma hope o ke kūʻai aku
Bioinformatics
Ka nānā 'ana i nā Gene Expression (DEGs).
Ka helu ʻana o ka ʻōlelo lncRNA - clustering
Hoʻonui i nā genes target lncRNA
ʻO ka loiloi kūlana mRNA a me lncRNA - Circos plot (ʻo ka pōʻai waena ka mRNA a ʻo ka cicrlce i loko ka lncRNA)
E ʻimi i nā holomua i hoʻokō ʻia e BMKGene's lncRNA equencing services ma o ka hōʻiliʻili ʻana o nā puke.
Ji, H. et al. (2020) 'Identification, functional prediction, and key lncRNA verification of cold stress-related lncRNAs in rats liver', Scientific Reports 2020 10:1, 10(1), pp. 1–14. doi: 10.1038/s41598-020-57451-7.
Jia, Z. et al. (2021) 'Integrative Transcriptomic Analysis Reveals the Immune Mechanism for a CyHV-3-Resistant Common Carp Strain', Frontiers in Immunology, 12, p. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.
Wang, XJ et al. (2022) 'Multi-Omics Integration-Based Prioritization of Cometing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Molecular Characteristics and Drug Candidates', Frontiers in Oncology, 12, p. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xiao, L. et al. (2020) 'Ka wehe 'ana o ka genetic coexpression network underlying photosynthesis in Populus', Plant Biotechnology Journal, 18(4), pp. 1015–1026. doi: 10.1111/PBI.13270.
Zheng, H. et al. (2022) 'A Global Regulatory Network for Dysregulated Gene Expression and Abnormal Metabolic Signaling in Immune Cells in the Microenvironment of Graves' Disease and Hashimoto's Thyroiditis', Frontiers in Immunology, 13, p. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.
