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Nā huahana

Metabolomics

ʻO ka metabolome ka huahana hope loa o ka genome a loaʻa iā ia ka hoʻopiha piha ʻana o nā molekala kaumaha haʻahaʻa haʻahaʻa (metabolites) i loko o ke kelepona, ʻiʻo, a i ʻole ke kino.Manaʻo ka Metabolomics e ana i ka laula o nā molekole liʻiliʻi i loko o ka pōʻaiapili o ka hoʻoulu kino a i ʻole nā ​​kūlana maʻi.Hāʻule nā ​​ʻano hana Metabolomics i ʻelua pūʻulu ʻokoʻa: nā metabolomics ʻole i hoʻopaʻa ʻia, kahi loiloi piha i manaʻo ʻia o nā analytes hiki ke ana i loko o kahi laʻana me nā mea ʻike ʻole kemika me ka hoʻohana ʻana i ka GC-MS/LC-MS, a me nā metabolomics i hoʻopaʻa ʻia, ke ana o nā pūʻulu i wehewehe ʻia o nā mea kemika. nā metabolites i hōʻike ʻia me ka biochemically.


Nā kikoʻī lawelawe

Nā Koina Laʻana

ʻO nā metabolomics i hoʻopaʻa ʻole ʻiaNā ʻano laʻana

Hoʻohālike ʻianui

Hoʻopiʻi Biological

LC-MS/GC-MSnā metabolomic ʻole i hoʻopaʻa ʻia

Nā lole

200mg

Mea kanu ≥6;

Holoholona ≥10;

Kanaka ≥30;

Plasma/serum

200ul

Kokohu Fecal/Intestinal

150mg

Nā pūnaewele

1*106

mimi

500ul

ʻO ka wai Rumen

1ml

ʻO nā metabolomic i manaʻo ʻiaNā ʻano laʻana

Manaʻo nui hāpana

Hoʻopiʻi Biological

Phytohormone/Tryptophane/ʻO ka hoʻoheheʻe ʻana o ka ikehu/ ʻO nā lipid i hoʻoheheʻe ʻia/ Carotenoid;carotinoid

Nā lole

500mg

Mea kanu ≥3;

Holoholona ≥6;

Plasma/serum

500ul

Fecal

1000mg

Nā pūnaewele

1*107

Nā kikoʻī lawelawe

ʻAʻole i hoʻopaʻa ʻia ka metabolomics:
1.ʻenehana makua a paʻa, hoʻonā kiʻekiʻe a me ke koho maikaʻi
2. Hoʻopiha piha i ka waihona ;
3. He kūpono no ka nānā ʻana i nā matrices paʻakikī, e hāʻawi ana i ka ʻike piha i hoʻokahi kānana;

ʻO nā metabolomic i manaʻo ʻia:
1.E hoʻohana i ka isotope i loko o ka hōʻoia maʻamau e hoʻomaikaʻi i ka qualitative a me ka quantitative pololei o nā mea;
2.Quality control system e hōʻoia i ka hilinaʻi o ka ʻikepili.

Holo Hana Hana

1.Non-targeted metabolomics

11

2. ʻO nā metabolomics i koho ʻia

22

3.Widely i hoʻopaʻa 'ia metabolomics

33

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