Hoʻokaʻina Metatranscriptome
Nā hiʻohiʻona lawelawe
● rRNA depletion ukali me directional mRNA waihona waihona.
● Ke kaʻina hana ma Illumina NovaSeq.
Pono lawelawe
●E aʻo i nā Hoʻololi o nā Kaiāulu Microbial Paʻakikī:Hana ʻia kēia ma ka pae transcriptional a ʻimi i nā genes hou.
●Ka wehewehe ʻana i nā hui kaiaulu Microbial me ka mea hoʻokipa a i ʻole ke kaiapuni.
●ʻIkepili Bioinformatic Comprehensive: Hāʻawi kēia i nā ʻike e pili ana i ka ʻauhau kaiāulu a me nā haku hana, a me ka nānā ʻana i ka hōʻike ʻano genes.
●Hōʻike Gene Nui:Ke hoʻohana nei i nā ʻikepili hana gene i kēia manawa no ka ʻike ʻike hōʻike gene o nā kaiāulu microbial.
●Kākoʻo ma hope o ke kūʻai ʻana:ʻO kā mākou kūpaʻa e ʻoi aku ma mua o ka hoʻopau ʻana o ka papahana me kahi 3 mau mahina ma hope o ke kūʻai aku ʻana. I kēia manawa, hāʻawi mākou i ka hahai ʻana o ka papahana, kōkua hoʻoponopono, a me nā hālāwai Q&A e hoʻoponopono i nā nīnau pili i nā hopena.
Nā kikoʻī lawelawe
| Anuu hoʻonohonoho | Hoʻolālā Sequencing | Manaʻo ʻia ka ʻikepili | Ka Mana Mana o ka Ikepili |
| ʻO Illumina NovaSeq | PE150 | 12Gb | Q30≥85% |
Nā Koina Laʻana
| Hoʻopaʻa (ng/µL) | huina nui (µg) | Volume (µL) | OD260/280 | OD260/230 | RIN |
| ≥50 | ≥1.0 | ≥20 | 1.8-2.0 | 1.0-2.5 | ≥6.5 |
Holo Hana Hana
Hāʻawi laʻana
Kūkulu hale waihona puke
Ke kaʻina ʻana
ʻIkepili ʻikepili
Nā lawelawe ma hope o ke kūʻai aku
Loaʻa i kēia ka nānā ʻana:
● Hoʻoponopono i ka maikaʻi o ka ʻikepili
● Hui Palapala
● Hōʻike ʻauhau a me ka nui
● Hōʻike Hana a me ka Nui
● Ka helu ʻana a me ka ʻike ʻokoʻa
Māhele ʻauhau o kēlā me kēia hāpana:
ʻIke ʻokoʻa Beta: UPGMA
Hōʻike kikoʻī - He nui ka GO
Ka nui ʻauhau ʻokoʻa - LEFSE
E ʻimi i nā holomua i hoʻolako ʻia e BMKGene's meta transcriptomic sequencing services ma o ka hōʻiliʻili ʻana o nā puke.
Lu, Z. et al. (2023) ʻO ka hoʻomanawanui ʻana o ka waika o ka bacteria hoʻohana i ka lactate o ke kauoha Bacteroidales kōkua i ka pale ʻana i ka acidosis ruminal i nā kao i hoʻololi ʻia i kahi meaʻai kiʻekiʻe-concentrate',Mea ai holoholona, 14, aoao 130–140. doi: 10.1016/J.ANINU.2023.05.006.
Mele, Z. et al. (2017) 'Unraveling core functional microbiota in traditional solid-state fermentation by high-throughput amplicons and metatranscriptomic sequencing',Nā palena i ka Microbiology, 8(IULAI). doi: 10.3389/FMICB.2017.01294/PIHA.
Wang, W. et al. (2022) 'ʻIke ʻia nā Mycoviruses mai kahi Metatranscriptomics Survey of the Phytopathogenic Alternaria Fungus',Virus, 14(11), p. 2552. doi: 10.3390/V14112552/S1.
Wei, J. et al. (2022) 'Hōʻike ka loiloi metatranscriptome parallel i ka hoʻohaʻahaʻa ʻana o nā metabolites kiʻekiʻe o nā mea kanu e nā pipi a me ko lākou ʻōpū ʻōpū',Molekala Kaiaola, 31(15), pp. 3999–4016. doi: 10.1111/MEC.16557.
