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Nā huahana

Hoʻokaʻina Metatranscriptome

ʻO ka metatranscriptome sequencing e hōʻike ana i ka hōʻike gene o nā microbes (ʻo nā eukaryotes a me nā prokaryotes) i loko o nā wahi kūlohelohe (ʻo ia hoʻi ka lepo, ka wai, ke kai, nā feces, a me ka ʻōpū.). o nā ʻano, ka loiloi hoʻonui hana o nā ʻano genes i hōʻike ʻia, a me nā mea hou aku.

Papahana: Illumina NovaSeq Platform


Nā kikoʻī lawelawe

Nā Koina Laʻana

Nā ʻano laʻana: Total RNA;

Ka nui: 2.5ug;

Kūlana: 50ng / ul;

Hoʻolālā lawelawe

ʻAno waihona: 250-300bp hoʻokomo i ka waihona cDNA;

Ka Papahana a me ka Papahana: illumina NovaSeq Platform;paired-end 150bp;

Manaʻo ʻia ka ʻikepili ʻikepili: 12G raw data / sample

Holo Hana Hana

hāʻawi laʻana

Hāʻawi laʻana

Hoʻomākaukau hale waihona puke

Kūkulu hale waihona puke

Ke kaʻina ʻana

Ke kaʻina ʻana

ʻIkepili ʻikepili

ʻIkepili ʻikepili

Mahope o ke kuai ana

Nā lawelawe ma hope o ke kūʻai aku


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