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Hoʻokaʻina Metatranscriptome
ʻO ka metatranscriptome sequencing e hōʻike ana i ka hōʻike gene o nā microbes (ʻo nā eukaryotes a me nā prokaryotes) i loko o nā wahi kūlohelohe (ʻo ia hoʻi ka lepo, ka wai, ke kai, nā feces, a me ka ʻōpū.). o nā ʻano, ka loiloi hoʻonui hana o nā ʻano genes i hōʻike ʻia, a me nā mea hou aku.
Papahana: Illumina NovaSeq Platform
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Fungal Genome
Hāʻawi ʻo Biomarker Technologies i ka loiloi genome, genome maikaʻi a me ka genome pene-complete o ka fungal ma muli o ka pahuhopu noiʻi kikoʻī.Hiki ke hoʻokō ʻia ke kaʻina o Genome, ka hui ʻana a me ka hōʻike hana ma o ka hoʻohui ʻana i ke kaʻina o ka hanauna aʻe + ʻO ke kaʻina o ka hanauna ʻekolu e hoʻokō i ka hui genome kiʻekiʻe.Hiki ke hoʻohana ʻia ka ʻenehana Hi-C e hoʻomaʻamaʻa i ka hui genome ma ka pae chromosome.
Papahana:PacBio Sequel II
Nanopore PromethION P48
ʻO Illumina NovaSeq Platform
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Mea paahana
ʻO BMKCloud kahi papahana bioinformatic alakaʻi e hāʻawi ana i ka hopena hoʻokahi no nā papahana genomic, kahi i hilinaʻi nui ʻia e nā mea noiʻi ma nā ʻano ʻano like ʻole me ka lāʻau lapaʻau, ka mahiʻai, ka nohona, a me nā mea ʻē aʻe. , punawai helu, waihona lehulehu, bioinformtic online courses, etc. BMKCloud he mau mea hana bioinformatic i hoohana pinepine ia e like me ka gene annotation, evolutionary genetic tools, ncRNA, data quality control, assembly, alignment, data extraction, mutations, statistics, figure generator, sequence. kālailai, etc.
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ʻO Genome Paʻa ʻo Bacteria
Hāʻawi ʻo Biomarker Technologies i ka lawelawe sequencing ma ke kūkulu ʻana i ka genome piha o ka bacteria me ka ʻole o ke āpau.ʻO ke kaʻina hana nui o ka bacteria e hoʻopau ana i ka hana genome e pili ana i ke kaʻina o ka hanauna ʻekolu, ka hui ʻana, ka annotation hana a me ka loiloi bioinformatic holomua e hoʻokō nei i nā pahuhopu noiʻi kikoʻī.ʻO ka hōʻike piha ʻana o ka genome bacteria e hāʻawi i ka mana i ka hōʻike ʻana i nā mīkini kumu i lalo o kā lākou mau kaʻina olaola, hiki ke hāʻawi i kahi kuhikuhi waiwai no nā noiʻi genomic i nā ʻano eukaryotic kiʻekiʻe.
Papahana:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
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RNA liʻiliʻi
ʻO nā RNA liʻiliʻi he ʻano pōkole non-coding RNA me ka lōʻihi o 18-30 nt, me ka miRNA, siRNA a me ka piRNA.Ua hōʻike nui ʻia kēia mau RNA liʻiliʻi e pili ana i nā kaʻina hana ola like ʻole e like me ka hoʻohaʻahaʻa ʻana o ka mRNA, ka pāpā ʻana i ka unuhi, ka hoʻokumu ʻana o heterochromatin, a me nā mea ʻē aʻe. ʻO ka papa hana hoʻopaʻa ʻana kaʻina hana maʻamau a me ka ʻimi ʻikepili kiʻekiʻe.Ma ke kumu o ka ʻikepili RNA-seq, hiki i ka loiloi maʻamau ke hoʻokō i ka ʻike miRNA a me ka wānana, ka wānana gene target miRNA, annotation a me ka nānā ʻana i ka ʻōlelo.Hiki i ka loiloi holomua ka huli ʻana a me ka unuhi ʻana o ka miRNA, ka hana kiʻi Venn, ka miRNA a me ka kūkulu ʻana i ka ʻoihana gene.
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NGS-WGS (Illumina/BGI)
ʻO NGS-WGS kahi kahua hōʻano hou genome re-sequencing ana, i hoʻomohala ʻia ma ke kumu o ka ʻike waiwai ma Biomarker Technologies.Hāʻawi kēia paepae maʻalahi i ka hoʻouna wikiwiki ʻana i kahi kaila hana hoʻopili i hoʻohui ʻia ma ka hoʻonohonoho ʻana i kekahi mau ʻāpana kumu, i kūpono no ka ʻikepili sequencing DNA i hana ʻia mai ka paepae Illumina a me BGI sequencing platform.Hoʻokomo ʻia kēia kahua ma luna o ka server computing hana kiʻekiʻe, e hāʻawi ana i ka mana o ka nānā ʻana i ka ʻikepili kūpono loa i ka manawa pōkole loa.Loaʻa ka mining data pilikino ma ke kumu o ka nānā ʻana maʻamau, me ka huli ʻana i nā gene mutated, PCR primer design, etc.
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mRNA(Hōʻike)
ʻO Transcriptome ka loulou ma waena o ka ʻike genetic genomic a me ka proteome o ka hana biological.ʻO ka hoʻoponopono pae transcription ka mea koʻikoʻi a ʻo ke ʻano hoʻoponopono i aʻo nui ʻia o nā meaola.Hiki i ka transcriptome sequencing ke hoʻonohonoho i ka transcriptome i kēlā me kēia manawa i ka manawa a i ʻole ma lalo o kekahi kūlana, me ka hoʻoholo pololei i ka nucleotide hoʻokahi. laʻana i nā palapala kikoʻī.
I kēia manawa, ua hoʻohana nui ʻia ka ʻenehana sequencing transcriptome i ka agronomy, ka lāʻau lapaʻau a me nā ʻano noiʻi ʻē aʻe, me ka hoʻoponopono ʻana i nā holoholona a me nā mea kanu, ka hoʻoponopono ʻana i ke kaiapuni, ka launa pū ʻana, ka localization gene, species genetic evolution and tumor and genetic disease detection.
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Metagenomics (NGS)
Hoʻolālā ʻia kēia kahua hoʻopaʻa inoa no ka ʻikepili ʻikepili metagenomic shotgun ma ke kumu o nā makahiki o ka ʻike.Loaʻa iā ia ke kahe hana i hoʻohui ʻia i loko o nā ʻano loiloi metagenomics maʻamau e pono ai e pili ana i ka hoʻoili ʻana i ka ʻikepili, nā haʻawina pae ʻano, nā haʻawina pae hana gene, metagenome binning, a me nā mea ʻē aʻe. , hoʻonohonoho hoʻonohonoho ʻāpana, hana kiʻi pilikino, etc.
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LncRNA
ʻO nā RNA non-coding lōʻihi (lncRNA) kekahi ʻano o nā transcript me ka lōʻihi ma mua o 200 nt, ʻaʻole hiki ke hoʻopaʻa i nā protein.Hōʻike nā hōʻike hōʻuluʻulu e hiki ke hana ka hapa nui o nā lncRNA.Hiki iā mākou ke hōʻike i nā kaʻina lncRNA a me ka hoʻonohonoho ʻana i ka ʻike a alakaʻi iā mākou e ʻike i nā lncRNA me nā hana hoʻoponopono koʻikoʻi.Haʻaheo ʻo BMKCloud e hāʻawi i kā mākou mea kūʻai aku lncRNA sequencing analysis platform e hoʻokō i ka loiloi lncRNA wikiwiki, hilinaʻi a maʻalahi.
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GWAS
ʻO ka Genome-wide association study (GWAS) ka manaʻo e ʻike i nā ʻano ʻano genetic (genotype) e pili ana i nā ʻano kikoʻī (phenotype).Ke noiʻi nei nā haʻawina GWA i nā mea hōʻailona genetic e hele i ka genome holoʻokoʻa o ka heluna nui o nā kānaka a wānana i nā hui genotype-phenotype ma o ka nānā ʻana helu helu ma ka pae heluna kanaka.Hiki ke ʻike i nā ʻano genetic āpau i ka resequencing holoʻokoʻa.I ka hui pū ʻana me ka ʻikepili phenotypic, hiki ke hana ʻia ʻo GWAS e ʻike i nā SNP pili phenotype, QTL a me nā genes moho, e kākoʻo ikaika ana i ka hoʻohua holoholona/mea kanu o kēia wā.ʻO SLAF kahi hoʻolālā hoʻolālā genome maʻalahi i hoʻomohala ʻia, kahi e ʻike ai i nā māka hoʻolaha genome-ākea, SNP.ʻO kēia mau SNP, ma ke ʻano he molecular genetic markers, hiki ke hana ʻia no nā haʻawina hui me nā ʻano i kuhikuhi ʻia.He hoʻolālā kumu kūʻai i ka ʻike ʻana i nā ʻano paʻakikī e pili ana i nā ʻano like ʻole.
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Nanopore Ka lōʻihi lōʻihi
ʻO nā isoforms paʻakikī a me nā ʻano like ʻole i loko o nā mea ola he mau ʻano genetic koʻikoʻi no ka hoʻoponopono ʻana i ka hōʻike gene a me ka ʻokoʻa protein.ʻO ka ʻike pololei ʻana i nā hale transcript ke kumu no ka noiʻi hohonu ʻana i nā ʻano hoʻoponopono hoʻoponopono hōʻike gene.Ua hoʻokō maikaʻi ʻo Nanopore sequencing platform i ka haʻawina transcriptomic i ka pae isoform.Hoʻolālā ʻia kēia kahua hoʻopaʻa ʻana e kālailai i ka ʻikepili RNA-Seq i hana ʻia ma ke kahua Nanopore ma ke kumu o ka genome reference, kahi e loaʻa ai nā loiloi qualitative a me quantitative ma nā pae gene a me nā pae transcripts.
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pōʻai-RNA
ʻO ka Circular RNA(circRNA) kekahi ʻano o ka RNA non-coding, i ʻike ʻia i kēia manawa e pāʻani i ke kuleana koʻikoʻi i nā ʻoihana hoʻoponopono e pili ana i ka hoʻomohala ʻana, ka pale ʻana i ke kaiapuni, a pēlā aku. Hoʻohui ʻia nā ʻaoʻao o ka circRNA e hana i kahi ʻano pōʻai, e hoʻopakele iā lākou mai ka ʻeli ʻana o exonuclease a ʻoi aku ka paʻa ma mua o ka hapa nui o ka RNA linear.Ua ʻike ʻia ʻo CircRNA he mau hana like ʻole i ka hoʻoponopono ʻana i ka hōʻike gene.Hiki i ka CircRNA ke hana ma ke ʻano he ceRNA, nāna e hoʻopaʻa i ka miRNA me ka hoʻokūkū, i kapa ʻia ʻo miRNA sponge.Hāʻawi ka CircRNA sequencing analysis platform i ka hoʻolālā circRNA a me ka nānā ʻana i ka hōʻike, ka wānana pahuhopu a me ka loiloi hui pū me nā ʻano mole RNA ʻē aʻe.
