Hoʻemi ʻia ka hoʻohālikelike Bisulfite Sequencing (RRBS)
Nā kikoʻī lawelawe
Papahana: NovaSeq paepae, PE150
ʻAno waihona: 200-400bp hoʻokomo i ka waihona DNA i mālama ʻia i ka bisulfite
Hoʻolālā kaʻina hana: Paired-End 150 bp
Manaʻo i ka hoʻopuka ʻikepili: 10 Gb ʻikepili maka/lapana
Nā Koina Laʻana
Ka nui DNA: ≥ 2ug
Hoʻopili DNA: ≥ 20ng / μl.
Maʻemaʻe: OD260/280 = 1.8 a 2.0 me ka hoʻohaʻahaʻa ʻole a i ʻole RNA contamination
ʻIkepili bioinformatics
Holo Hana Hana
Hāʻawi laʻana
Kūkulu hale waihona puke
Ke kaʻina ʻana
ʻIkepili ʻikepili
Nā lawelawe ma hope o ke kūʻai aku
a.Hōʻike i nā helu helu hoʻonohonoho genome
Heluhelu helu palapala palapala
Sʻikepili o ka hohonu o ka hoʻopili ʻana a me ka uhi ʻana o ka pūnaewele C
Ahoʻopili ʻia
Digestion pono o kela a me keia laana
b.ʻIke i ka pae methylation
Ka papa inoa o nā pae methylation ma ka pūnaewele C
BS hoʻololi
Mcytosine ethylated
Māhele pae methylation
c.Palapala methylation
Genome-ākea
Gelement enome
d.Hoʻohālikelike i nā pae methylation ma waena o nā laʻana
Pairwise Ka pilina o CG methylation
PCA-CG methylation
Clustering o CG methylation
d.Ka nānā ʻana i ka methylation ʻokoʻa
Sʻikepili o DMR
DMR puunaue loihi
Hʻai palapala ʻāina
DMāhele MR ma ka ʻāina i hoʻopaʻa ʻia
