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High definition 16s Rrna Gene Sequencing - Metagenomic Sequencing-Nanopore – Biomarker


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High definition 16s Rrna Gene Sequencing - Metagenomic Sequencing-Nanopore – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data quality control

ü Metagenome assembly

ü Non-redundant gene set and annotation

ü Species diversity analysis

ü Genetic function diversity analysis

ü Inter-group analysis

ü Association analysis against experimental factors

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Sample Requirements and Delivery

Sample requirements and delivery

Sample Requirements:   

For DNA extracts: 

Sample Type

Amount

Concentration

Purity

DNA extracts

> 30 ng

> 1 ng/μl

OD260/280= 1.6-2.5

For environmental samples:

Sample type

Recommended sampling procedure

Soil

Sampling amount: approx. 5 g; Remaining withered substance needs to be removed from surface; Grind large pieces and pass through 2 mm filter; Aliquot samples in sterile EP-tube or cyrotube for reservation.

Feces

Sampling amount: approx. 5 g; Collect and aliquot samples in sterile EP-tube or cryotube for reservation.

Intestinal contents

Samples need to be processed under aseptic condition. Wash collected tissue with PBS; Centrifuge the PBS and collect the precipitant in EP-tubes.

Sludge

Sampling amount: approx. 5 g; Collect and aliquot sludge sample in sterile EP-tube or cryotube for reservation

Waterbody

For sample with limited amount of microbial, such as tap water, well water, etc., Collect at least 1 L water and pass through 0.22 μm filter to enrich microbial on the membrane. Store the membrane in sterile tube.

Skin

Carefully scrape skin surface with sterile cotton swab or surgical blade and place it in sterile tube.

Recommended Sample Delivery

Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services


Product detail pictures:

High definition 16s Rrna Gene Sequencing -
 Metagenomic Sequencing-Nanopore – Biomarker detail pictures

High definition 16s Rrna Gene Sequencing -
 Metagenomic Sequencing-Nanopore – Biomarker detail pictures

High definition 16s Rrna Gene Sequencing -
 Metagenomic Sequencing-Nanopore – Biomarker detail pictures

High definition 16s Rrna Gene Sequencing -
 Metagenomic Sequencing-Nanopore – Biomarker detail pictures

High definition 16s Rrna Gene Sequencing -
 Metagenomic Sequencing-Nanopore – Biomarker detail pictures

High definition 16s Rrna Gene Sequencing -
 Metagenomic Sequencing-Nanopore – Biomarker detail pictures

High definition 16s Rrna Gene Sequencing -
 Metagenomic Sequencing-Nanopore – Biomarker detail pictures


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  • Timely delivery, strict implementation of the contract provisions of the goods, encountered special circumstances, but also actively cooperate, a trustworthy company!
    5 Stars By Coral from Uruguay - 2017.03.07 13:42
    The factory technical staff not only have high level of technology, their English level is also very good, this is a great help to technology communication.
    5 Stars By Marjorie from Adelaide - 2018.11.06 10:04

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