16S/18S/ITS Amplicon Sequencing-PacBio
Cov yam ntxwv ntawm kev pabcuam
● Lub platform txheeb xyuas cov ntaub ntawv: PacBio Revio
● Hom kev txheeb xyuas: CCS (Nyeem HiFi)
● Kev ua kom lub cheeb tsam tsom mus rau qhov kev ua raws li kev sib txuas ntawm cov amplicons ua ntej kev npaj HiFi SMRT bell library
Cov Kev Pabcuam Zoo
●Kev daws teeb meem siab dua ntawm taxonomic: Tkev txheeb xyuas luv luv ntawm han,ua rau muaj kev faib tawm OTU siab dua ntawm qib hom tsiaj.
●Kev hu xov tooj raug heevKev txheeb xyuas hom PacBio CCS (nyeem HiFi).
●Tsis muaj kev cais tawmKev txheeb xyuas sai ntawm cov kab mob me me hauv cov qauv ib puag ncig.
●Siv tau davKev tshawb fawb txog cov kab mob sib txawv.
●Kev tshuaj xyuas bioinformatic kom ntxawsCov pob khoom QIIME2 tshiab kawg (kev nkag siab txog kev ntsuas ntau yam hauv kev ua neej ntawm cov kab mob me me) nrog ntau yam kev tshuaj xyuas ntawm cov ntaub ntawv, cov lus piav qhia, OTU/ASV.
●Kev Paub Ntau YamBMKGENE muaj ntau txhiab qhov project amplicon sequencing uas tau ua txhua xyoo, thiab muaj ntau tshaj kaum xyoo ntawm kev paub dhau los, pab pawg neeg tshuaj xyuas uas muaj kev txawj ntse heev, cov ntsiab lus dav dav, thiab kev txhawb nqa tom qab muag zoo heev.
Cov Lus Qhia Txog Kev Pabcuam
| Tsev qiv ntawv | Txoj Kev Npaj Txog Sequencing | Cov ntaub ntawv pom zoo |
| Amplicon | PacBio Revio | 10/30/50 K daim ntawv cim npe (CCS) |
Cov Qauv Yuav Tsum Tau Ua
| Kev Ntsuas (ng/µL) | Tag nrho cov nqi (µg) | Ntim (µL) |
| ≥5 | ≥0.3 | ≥20 |
Kev Xa Qauv Pom Zoo
Khov cov qauv hauv cov kua nitrogen rau 3-4 teev thiab khaws cia rau hauv cov kua nitrogen lossis -80 degree kom khaws cia ntev. Yuav tsum xa cov qauv nrog dej khov qhuav.
Kev Ua Haujlwm Pabcuam
Kev xa khoom qauv
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Cov kev pab cuam tom qab muag
Muaj xws li cov kev tshuaj xyuas hauv qab no:
●Kev tswj xyuas cov ntaub ntawv raw
●OTU clustering/De-noise (ASV)
● Cov lus piav qhia OTU
●Kev tshuaj xyuas ntau haiv neeg ntawm Alpha: ntau cov ntsuas, suav nrog Shannon, Simpson thiab ACE.
●Kev tshuaj xyuas ntau haiv neeg ntawm Beta
●Kev tshuaj xyuas ntawm cov pab pawg neeg
●Kev tshuaj xyuas kev sib raug zoo: ntawm cov yam ntxwv ib puag ncig thiab OUT cov khoom sib xyaw thiab ntau haiv neeg
●16S kev kwv yees txog kev ua haujlwm ntawm cov noob caj noob ces
Histogram ntawm kev faib tawm ntawm taxonomic
Tsob ntoo phylogenetic faib tawm hauv zej zog
Kev tshuaj xyuas ntau haiv neeg ntawm Alpha: ACE
cov ntawv qhia
Kev tshuaj xyuas ntau haiv neeg ntawm Beta: PCoA
Kev tshuaj xyuas ntawm pawg neeg sib txawv: ANOVA
Tshawb nrhiav kev nce qib uas tau pab txhawb los ntawm BMKGene cov kev pabcuam amplicon sequencing nrog PacBio los ntawm kev sau cov ntawv tshaj tawm.
Gao, X. thiab Wang, H. (2023) 'Kev Tshawb Fawb Sib Piv ntawm Rumen Bacterial Profiles thiab Functions thaum lub sijhawm Adaptation rau Txawv Phenology (Regreen vs. Grassy) hauv Alpine Merino Yaj nrog Ob Theem Loj Hlob ntawm Alpine Meadow', Fermentation, 9(1), p. 16. doi: 10.3390/FERMENTATION9010016/S1.
Li, S. et al. (2023) 'Kev ntes cov khoom tsaus ntuj microbial hauv cov av suab puam siv cov metagenomics raws li culturomics thiab kev tshuaj xyuas siab', npj Biofilms thiab Microbiomes 2023 9:1, 9(1), pp. 1–14. doi: 10.1038/s41522-023-00439-8.
Mu, L. et al. (2022) 'Cov teebmeem ntawm cov ntsev fatty acid rau cov yam ntxwv fermentation, ntau haiv neeg ntawm cov kab mob thiab kev ruaj khov ntawm cov silage sib xyaw ua ke nrog alfalfa, cov quav ciab mov thiab cov nplej bran', Journal of the Science of Food and Agriculture, 102(4), pp. 1475–1487. doi: 10.1002/JSFA.11482.
Yang, J. et al. (2023) 'Kev Sib Cuam Tshuam ntawm Oxidative Stress Biomarkers thiab Gut Microbiota hauv Cov Kev Cuam Tshuam Antioxidant ntawm Cov Extracts los ntawm Sonchus brachyotus DC. hauv Oxazolone-Induced Intestinal Oxidative Stress hauv Adult Zebrafish', Antioxidants 2023, Vol. 12, Nplooj Ntawv 192, 12(1), p. 192. doi: 10.3390/ANTIOX12010192.
