Kev Rov Ua Dua Tshiab ntawm Cov Kab Mob Bacterial thiab Fungal Tag Nrho Genome
Cov haujlwm rov ua dua tshiab ntawm cov kab mob bacterial thiab fungal tag nrho yog qhov tseem ceeb rau kev txhim kho cov noob caj noob ces microbial los ntawm kev ua kom tiav thiab sib piv cov noob caj noob ces microbial. Qhov no pab txhawb kev tsim kho fermentation, kev ua kom zoo dua ntawm cov txheej txheem kev lag luam, thiab kev tshawb nrhiav cov txheej txheem metabolism thib ob. Ntxiv mus, kev rov ua dua tshiab ntawm cov kab mob fungal thiab bacterial yog qhov tseem ceeb rau kev nkag siab txog kev hloov pauv ib puag ncig, kev ua kom zoo dua cov kab mob, thiab qhia txog kev hloov pauv ntawm cov noob caj noob ces, nrog rau kev cuam tshuam dav hauv kev kho mob, kev ua liaj ua teb, thiab kev tshawb fawb ib puag ncig.
Cov Kev Pabcuam Zoo
●Kev Tshawb Fawb Txog Bioinformatic suav nrog Kev Hu Xov Tooj Txawv:Muab kev nkag siab txog kev ua haujlwm rau hauv cov genomes uas tau rov ua dua tshiab.
● Kev Paub Ntau YamPeb muaj ntau txhiab qhov project kho cov kab mob me me uas tau ua txhua xyoo, peb muaj ntau tshaj kaum xyoo ntawm kev paub dhau los, pab pawg neeg tshuaj xyuas uas muaj kev txawj ntse heev, cov ntsiab lus dav dav, thiab kev txhawb nqa tom qab muag zoo heev.
●Kev Txhawb Nqa Tom Qab Muag Khoom:Peb txoj kev cog lus tseem ceeb tshaj qhov ua tiav qhov project nrog rau 3 lub hlis kev pabcuam tom qab muag. Thaum lub sijhawm no, peb muab kev saib xyuas project, kev pab daws teeb meem, thiab cov lus nug thiab lus teb los teb cov lus nug ntsig txog cov txiaj ntsig.
Cov Lus Qhia Txog Kev Pabcuam
| Lub platform txheeb xyuas cov ntaub ntawv | Txoj Kev Npaj Txog Sequencing | Cov ntaub ntawv pom zoo | Kev tswj xyuas zoo |
| Illumina NovaSeq | PE150 | Qhov tob ntawm 100x | Q30≥85% |
Cov Qauv Yuav Tsum Tau Ua
| Kev Ntsuas (ng/µL) | Tag nrho cov nyiaj (ng) | Ntim (µL) |
| ≥1 | ≥60 | ≥20 |
Cov kab mob: ≥1x107 cov hlwb
Cov kab mob fungi ib lub hlwb: ≥5x106-1x107 cov hlwb
Macro Fungi: ≥4g
Kev Ua Haujlwm Pabcuam
Kev xa khoom qauv
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Cov kev pab cuam tom qab muag
Muaj xws li Kev Tshawb Fawb Hauv Qab No:
- Kev Tswj Xyuas Zoo ntawm Cov Ntaub Ntawv Sequencing
- Kev Sib Haum Xeeb Rau Cov Genome Siv
- Kev Hu Xov Tooj Sib Txawv: SNP thiab InDel
- Cov Lus Cim Txawv
Kev hu xov tooj sib txawv: hom SNP
Kev hu xov tooj sib txawv: Kev faib tawm qhov ntev ntawm InDel
Tshawb nrhiav cov kev nce qib uas tau pab txhawb los ntawm BMKGene cov kev pabcuam rov ua dua tshiab ntawm cov noob caj noob ces los ntawm kev sau cov ntawv tshaj tawm uas tau xaiv tseg.
Jia, Y. et al. (2023) 'Kev Sib Koom Tes Transcriptome thiab Tag Nrho Genome Re-Sequencing los Tshuaj Xyuas Cov Genes Tiv Thaiv Kab Mob rau Cov Nplej Dwarf Bunt',Phau ntawv xov xwm thoob ntiaj teb ntawm kev tshawb fawb molecular, 24(24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) 'Kev ua haujlwm ntawm cov piam thaj uas tswj los ntawm Ampicillin ua rau cov kab mob hloov pauv ntawm kev kam rau siab mus rau kev tiv taus',Kev Txhim Kho ntawm Kev Tshawb Fawb, 9(10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) 'Aliidiomarina halalkaliphila sp. nov., ib hom kab mob haloalkaliphilic cais los ntawm ib lub pas dej qab zib hauv Inner Mongolia Autonomous Region, Suav teb', Int. J. Syst. Evol.MicrobiolIb., 72, p. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. thiab Wang, G.-H. (2024) 'Genome sequence ntawm Staphylococcus nepalensis ZZ-2023a, cais tawm ntawm Nasonia vitripennis',Cov Lus Tshaj Tawm Txog Kev Pab Cuam Txog Kab Mob Microbiology. doi: 10.1128/MRA.00802-23.
