Cov kab mob thiab cov kab mob fungal tag nrho genome Re-Sequencing
Cov kab mob thiab cov kab mob fungal tag nrho-genome re-sequencing tej yaam num yog qhov tseem ceeb rau kev nce qib microbial genomics los ntawm kev ua kom tiav thiab sib piv ntawm microbial genomes. Qhov no pab txhawb kev fermentation engineering, kev ua kom zoo ntawm cov txheej txheem kev lag luam, thiab tshawb nrhiav cov txheej txheem metabolic theem nrab. Tsis tas li ntawd, cov kab mob fungal thiab kab mob rov ua dua yog qhov tseem ceeb rau kev nkag siab txog kev hloov pauv ib puag ncig, txhim kho cov kab mob, thiab nthuav tawm cov noob caj noob ces hloov pauv, nrog rau kev cuam tshuam dav hauv tshuaj, kev ua liaj ua teb, thiab kev tshawb fawb ib puag ncig.
Kev Pabcuam Zoo
●Bioinformatic Analysis muaj xws li Variant Hu:Muab kev pom zoo ua haujlwm rau hauv cov genomes rov ua dua.
● Kev paub dav dav: Nrog ntau txhiab tus microbial re-sequencing tej yaam num ua txhua xyoo, peb coj ntau tshaj kaum xyoo ntawm kev paub, pab pawg neeg soj ntsuam zoo heev, cov ntsiab lus dav, thiab kev txhawb nqa zoo tom qab muag.
●Kev them nyiaj yug tom qab muag:Peb qhov kev cog lus txuas ntxiv dhau qhov kev ua tiav nrog rau 3 lub hlis tom qab kev muag khoom. Nyob rau lub sijhawm no, peb muab cov phiaj xwm taug qab, kev pab daws teeb meem, thiab Q&A ntu los daws cov lus nug ntsig txog cov txiaj ntsig.
Service Specifications
| Sequence platform | Sequence Strategy | Cov ntaub ntawv pom zoo | Kev tswj kom zoo |
| Illumina NovaSeq | PEB 150 | Qhov tob ntawm 100x | Q30≥85% |
Cov Qauv Xav Tau
| Concentration (ng / μL) | Tag nrho cov nyiaj (ng) | Ntim (µL) |
| ≥1 | ≥60 | ≥ 20 |
Cov kab mob: ≥1x107 hlwb
Unicellular Fungi: ≥5x106-1x107 hlwb
Macro fungi: ≥4g
Kev Pabcuam Ua Haujlwm Flow
Qauv xa tuaj
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
Xws li Kev Tshawb Fawb Hauv qab no:
- Sequencing Data Quality Control
- Alignment rau Reference Genome
- Hu rau Variant: SNP thiab InDel
- Variant Annotation
Kev hu xov tooj sib txawv: SNP hom
Kev hu xov tooj sib txawv: InDel ntev faib
Tshawb nrhiav cov kev nce qib uas tau pab los ntawm BMKGene's microbial genome rov ua cov kev pabcuam los ntawm kev sau cov ntawv tshaj tawm.
Jia, Y. et al. (2023) 'Combining Transcriptome and Whole Genome Re-Sequencing to Screen Disease Resistance Genes for Nplej Dwarf Bunt',International Journal of Molecular Sciences, 24(24). doi: 10.3390/IJMS242417356.
Jiang, M. et al. (2023) 'Ampicillin-tswj cov piam thaj metabolism hauv kev hloov pauv ntawm kev ua siab ntev mus rau cov kab mob,Science Advances, 9(10). doi: 10.1126/SCIADV.ADE8582/SUPPL_FILE/SCIADV.ADE8582_SM.PDF.
Yang, M. et al. (2022) 'Aliidiomarina halalkaliphila sp. Nov., tus kab mob haloalkaliphilic cais tawm ntawm lub pas dej soda hauv Inner Mongolia Autonomous Region, Tuam Tshoj', Int. J. Syst. Evol.Microbiol,,72, ib. 5263. doi: 10.1099/ijsem.0.005263.
Zhu, Z., Wu, R. and Wang, G.-H. (2024) 'Genome sequence of Staphylococcus nepalensis ZZ-2023a, cais los ntawm Nasonia vitripennis',Cov Ntawv Tshaj Tawm Txog Microbiology. doi: 10.1128/MRA.00802-23.
