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PacBio-Full-length Transcriptome (Tsis Siv Neeg)
Amplicon (16S / 18S / ITS) platform yog tsim los nrog ntau xyoo ntawm kev paub nyob rau hauv microbial ntau haiv neeg qhov project tsom xam, uas muaj cov qauv kev soj ntsuam thiab tus kheej tsom xam: cov kev ntsuam xyuas yooj yim suav nrog cov ntsiab lus tseem ceeb ntawm kev tshawb fawb microbial tam sim no, cov ntsiab lus tsom xam yog nplua nuj thiab ua tiav, thiab cov txiaj ntsig kev txheeb xyuas tau nthuav tawm hauv daim ntawv qhia txog qhov project;Cov ntsiab lus ntawm kev txheeb xyuas tus kheej yog ntau haiv neeg.Cov qauv tuaj yeem xaiv tau thiab tsis tuaj yeem teeb tsa yooj yim raws li daim ntawv qhia kev txheeb xyuas thiab kev tshawb fawb lub hom phiaj, kom paub txog cov kev xav tau ntawm tus kheej.Windows operating system, yooj yim thiab ceev.
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PacBio-Full-length Transcriptome (Tsis Siv Neeg)
Noj Pacific Biosciences (PacBio) Isoform sequencing cov ntaub ntawv raws li kev tawm tswv yim, App no muaj peev xwm txheeb xyuas cov ntawv sau ua ntu zus (tsis muaj kev sib dhos).Los ntawm daim ntawv qhia tag nrho-ntev ib ntus tawm tsam kev siv genome, cov ntawv sau tuaj yeem ua kom zoo los ntawm cov noob caj noob ces, cov ntawv sau tseg, cov cheeb tsam coding, thiab lwm yam. Nyob rau hauv rooj plaub no, kev txheeb xyuas qhov tseeb dua ntawm mRNA cov qauv, xws li lwm txoj kev sib txuas, thiab lwm yam, tuaj yeem ua tiav.Kev sib koom ua ke nrog NGS cov ntaub ntawv teev cov ntaub ntawv sau ua ke ua rau muaj ntau cov lus piav qhia thiab kev txheeb xyuas qhov tseeb dua hauv kev qhia ntawm qib ntawv sau, uas feem ntau tau txais txiaj ntsig zoo hauv nqe lus sib txawv thiab kev tshuaj xyuas ua haujlwm.
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Cov cuab yeej
BMKCloud yog ib lub chaw bioinformatic uas muab ib qho kev daws teeb meem rau genomic cov kev pab cuam, uas yog dav ntseeg los ntawm cov kws tshawb fawb nyob rau hauv ntau yam arena nrog rau kev kho mob, kev ua liaj ua teb, ib puag ncig, thiab lwm yam. BMKCloud tau cog lus los muab kev koom ua ke, txhim khu kev qha thiab muaj txiaj ntsig cov kev pabcuam suav nrog bioinformatic tsom xam platforms thiab cov cuab yeej. , suav cov peev txheej, cov ntaub ntawv pej xeem, cov chav kawm bioinformtic online, thiab lwm yam BMKCloud muaj ntau yam nquag siv bioinformatic cov cuab yeej xws li gene annotation, evolutionary genetic tools, ncRNA, data quality control, assembly, alignment, data extraction, mutations, statistics, figure generator, sequence tsom xam, thiab lwm yam.
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me RNA
Cov RNA me me yog hom luv luv tsis-coding RNA nrog qhov nruab nrab ntev ntawm 18-30 nt, suav nrog miRNA, siRNA thiab piRNA.Cov RNA me me no tau raug tshaj tawm tias muaj kev koom tes hauv ntau yam kev lom neeg xws li mRNA degradation, translation inhibition, heterochromatin tsim, thiab lwm yam. SmallRNA sequencing tsom xam tau siv dav hauv kev tshawb fawb txog tsiaj / cog kev loj hlob, kab mob, kab mob, thiab lwm yam. sequencing tsom xam platform muaj cov txheej txheem tsom xam thiab qib siab cov ntaub ntawv mining.Raws li lub hauv paus ntawm RNA-seq cov ntaub ntawv, kev soj ntsuam tus qauv tuaj yeem ua tiav miRNA kev txheeb xyuas thiab kev kwv yees, miRNA lub hom phiaj kev kwv yees, annotation thiab kev tsom xam.Kev soj ntsuam qib siab ua rau muaj kev hloov kho miRNA tshawb nrhiav thiab rho tawm, Venn daim duab tiam, miRNA thiab lub hom phiaj gene network tsim.
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NWS-WGS (Illumina/BGI)
NGS-WGS yog tag nrho genome re-sequencing tsom xam platform, uas yog tsim los ntawm lub hauv paus ntawm kev nplua nuj nyob rau hauv Biomarker Technologies.Qhov yooj yim-rau-siv platform no tso cai rau kev xa tawm sai ntawm kev sib koom ua ke ntawm kev ua haujlwm los ntawm kev teeb tsa ob peb yam yooj yim, uas haum rau DNA sequencing cov ntaub ntawv tsim los ntawm ob qho tib si Illumina platform thiab BGI sequencing platform.Lub platform no yog siv los ntawm kev ua haujlwm siab ua haujlwm hauv computer server, uas txhawb nqa cov ntaub ntawv txheeb xyuas cov ntaub ntawv muaj txiaj ntsig zoo hauv lub sijhawm txwv.Tus kheej cov ntaub ntawv tsuas yog muaj nyob rau hauv lub hauv paus ntawm kev soj ntsuam tus qauv, nrog rau cov lus nug hloov pauv, PCR primer tsim, thiab lwm yam.
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mRNA(Reference)
Transcriptome yog qhov txuas ntawm cov ntaub ntawv genomic genetic thiab proteome ntawm kev ua haujlwm lom neeg.Transcriptional theem kev cai yog qhov tseem ceeb tshaj plaws thiab feem ntau kawm txog kev cai hom kab mob.Transcriptome sequencing tuaj yeem ua raws cov ntawv sau tseg ntawm txhua lub sijhawm lossis nyob rau hauv ib qho xwm txheej, nrog kev daws teeb meem raug rau ib qho nucleotide.Nws tuaj yeem cuam tshuam txog qib ntawm cov noob hloov pauv, ib txhij txheeb xyuas thiab txheeb xyuas cov ntaub ntawv tsis tshua muaj thiab ib txwm muaj, thiab muab cov ntaub ntawv tsim nyog ntawm piv txwv cov ntawv sau tseg.
Tam sim no, transcriptome sequencing thev naus laus zis tau siv dav hauv agronomy, tshuaj thiab lwm yam kev tshawb fawb, suav nrog kev tswj hwm tsiaj thiab cog kev loj hlob, kev hloov pauv ib puag ncig, kev tiv thaiv kab mob, kab mob hauv cheeb tsam, hom caj ces evolution thiab qog thiab kuaj kab mob caj ces.
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Metagenomics (NGS)
Qhov kev tshuaj ntsuam xyuas platform no yog tsim los rau kev tshawb xyuas cov ntaub ntawv phom phom metagenomic raws li kev paub ntau xyoo.Nws muaj kev sib xyaw ua haujlwm uas muaj ntau yam kev xav tau metagenomics tsom xam suav nrog kev ua cov ntaub ntawv, hom kev tshawb fawb qib, kev tshawb fawb txog qib kev ua haujlwm, metagenome binning, thiab lwm yam. Tsis tas li ntawd, cov cuab yeej kho cov ntaub ntawv tsuas yog muaj nyob rau hauv cov qauv kev soj ntsuam kev ua haujlwm, suav nrog cov noob thiab hom lus nug. , parameter teeb tsa, tus kheej daim duab tsim, thiab lwm yam.
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LncRNA
Ntev tsis-coding RNAs (lncRNA) yog ib hom ntawv sau nrog ntev dua 200 nt, uas tsis tuaj yeem sau cov proteins.Cov ntaub ntawv pov thawj qhia tau tias feem ntau ntawm lncRNAs feem ntau yuav ua haujlwm.Cov thev naus laus zis dhau los ua ntu zus thiab cov cuab yeej tshawb fawb bioinformatic pab peb kom nthuav tawm lncRNA cov kab ke thiab muab cov ntaub ntawv qhia tau zoo dua thiab coj peb mus nrhiav lncRNAs nrog cov haujlwm tseem ceeb ntawm kev tswj hwm.BMKCloud zoo siab los muab peb cov neeg siv khoom lncRNA sequencing tsom xam platform kom ua tiav ceev, txhim khu kev qha thiab hloov pauv lncRNA tsom xam.
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GWAS
Genome-wide Association txoj kev tshawb fawb (GWAS) lub hom phiaj ntawm kev txheeb xyuas cov kab mob sib txawv (genotype) uas cuam tshuam nrog cov yam ntxwv tshwj xeeb (phenotype).GWA cov kev tshawb fawb tshawb xyuas cov cim caj ces hla tag nrho cov genome ntawm cov neeg coob coob thiab kwv yees cov koom haum genotype-phenotype los ntawm kev txheeb xyuas cov lej ntawm cov pej xeem.Tag nrho-genome resequencing tuaj yeem nrhiav pom txhua yam kab mob sib txawv.Kev sib txuas nrog cov ntaub ntawv phenotypic, GWAS tuaj yeem ua tiav los txheeb xyuas cov phenotype ntsig txog SNPs, QTLs thiab cov noob neeg sib tw, uas txhawb nqa cov tsiaj txhu niaj hnub / cog qoob loo.SLAF yog tus kheej-tsim simplified genome sequencing lub tswv yim, uas pom genome-wide faib cov cim, SNP.Cov SNPs no, raws li cov cim caj ces molecular, tuaj yeem ua tiav rau cov kev tshawb fawb koom nrog nrog cov hom phiaj.Nws yog ib qho txiaj ntsig zoo hauv kev txheeb xyuas cov yam ntxwv nyuaj uas cuam tshuam txog kev hloov pauv caj ces.
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Nanopore Full-length transcriptomics
Complex thiab hloov pauv lwm yam isoforms hauv cov kab mob yog cov txheej txheem tseem ceeb ntawm caj ces rau kev tswj cov noob qhia thiab ntau hom protein.Kev txheeb xyuas qhov tseeb ntawm cov qauv sau ntawv yog lub hauv paus rau kev kawm tob txog cov qauv kev cai ntawm cov noob caj noob ces.Nanopore sequencing platform tau ua tiav kev tshawb fawb transcriptomic rau isoform-theem.Qhov kev tsom xam platform no yog tsim los tshuaj xyuas RNA-Seq cov ntaub ntawv tsim tawm ntawm Nanopore platform raws li kev siv genome, uas ua tiav cov kev soj ntsuam zoo thiab ntau yam hauv ob theem noob thiab cov ntawv sau tseg.
