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Cov khoom

Chromatin Immunoprecipitation Sequencing (ChIP-seq)

Chip-Seq muab genome-wide profileing ntawm DNA lub hom phiaj rau kev hloov kho histone, hloov pauv cov ntsiab lus, thiab lwm cov proteins uas cuam tshuam nrog DNA.Nws muab cov kev xaiv ntawm chromatin immuno-precipitation (ChIP) kom rov qab tau cov protein-DNA complexes, nrog lub zog ntawm cov tiam tom ntej sequencing (NGS) rau high-throughput sequencing ntawm cov DNA zoo.Tsis tas li ntawd, vim hais tias cov protein-DNA complexes tau zoo los ntawm cov hlwb nyob, cov chaw sib txuas tuaj yeem muab piv rau ntau hom cell thiab cov ntaub so ntswg, lossis nyob rau hauv cov xwm txheej sib txawv.Cov ntawv thov sib txawv los ntawm kev tswj hwm kev hloov pauv mus rau txoj hauv kev loj hlob mus rau kab mob mechanisms thiab dhau mus.

Platform: Illumina NovaSeq Platform


Cov ntsiab lus kev pabcuam

Bioinformatics

Cov txiaj ntsig Demo

Kev Pabcuam Zoo

● Platform: Illumina NovaSeq Platform, PE150

● Lub tsev qiv ntawv hom: 100-500bp ntxig DNA lub tsev qiv ntawv (nyob ntawm qhov kev faib tawm siab tshaj)

● Sequencing lub tswv yim: Paired-End 150 bp

● Pom zoo cov ntaub ntawv tso zis: 6 Gb raw cov ntaub ntawv / qauv.

Cov Qauv Xav Tau

DNA ntau: ≥70 ng, lub ntsiab ncov ntawm 100 txog 500 bp

Qauv ntim: ≥ 10 μl

OD260/280 = 1.8 txog 2.0 yam tsis muaj degradation lossis RNA paug

Kev sim ua haujlwm

kev 1

Kev Pabcuam Ua Haujlwm Flow

coj mus kuaj

Qauv xa tuaj

Kev npaj tsev qiv ntawv

Kev tsim kho tsev qiv ntawv

Sequencing

Sequencing

Cov ntaub ntawv tsom xam

Cov ntaub ntawv tsom xam

Kev pab cuam tom qab muag

Kev pab cuam tom qab muag


  • Yav dhau los:
  • Tom ntej:

  • 表观-01

    1. Peak enrichment faib

    官网图1

     

    2. MA zajlus ntawm qhov sib txawv ncov

    官网图 2

     

    3.KEGG annotation

    kev 3

     

    4. GO kev faib tawm

    kev 4

    tau ib nqe lus

    Sau koj cov lus ntawm no thiab xa tuaj rau peb

    Xa koj cov lus rau peb: