Cov khoom

DNA / RNA Sequencing - Nanopore Sequencer

ONT sequencing yog ib qho molecule real-time hluav taws xob teeb liab sequencing tshuab raws li nanopores, sequencing txoj cai ntawm txhua lub platform yog tib yam.Ob-stranded DNA / RNA yuav khi rau nanoporous protein nyob rau hauv biofilm thiab unwinding nyob rau hauv cov hlau lead ntawm lub cev muaj zog protein, nyob rau hauv qhov kev txiav txim ntawm voltage txawv ntawm ob sab ntawm lub biofilm, DNA / RNA strands dhau los ntawm nanopore channel protein ntawm ib tug tej yam. tus nqi.Vim tias qhov sib txawv ntawm cov khoom siv tshuaj sib txawv ntawm cov hauv paus sib txawv ntawm DNA / RNA strand, thaum ib lub hauv paus los yog DNA molecule dhau los ntawm nanopore channel, nws yuav ua rau muaj kev hloov pauv ntawm cov hluav taws xob sib txawv.Los ntawm kev txheeb xyuas thiab sib raug rau cov teeb liab no, cov hom kev sib raug zoo tuaj yeem suav nrog, thiab kev tshawb nrhiav lub sijhawm tiag tiag ntawm cov kab ke tuaj yeem ua tiav.

Tiv tauj peb

Cov ntsiab lus kev pabcuam

Kev tshwm sim Demo

Cov ntsiab lus ntawm kev pabcuam

Platform	Lub tsev qiv ntawv loj	Theoretical Data Yield (Pib Cell)	Single-base Accuracy	Daim ntawv thov
Nanopor	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Cell	85-92%	SV hu, De novo, Full-length sequencing, Iso-Seq, Gene annotation, Tshawb nrhiav DNA methylation

Kev Pabcuam Zoo

● Tshaj li 5 xyoos kev paub dhau los ntawm PacBio sequencing platform nrog ntau txhiab txoj haujlwm kaw nrog ntau hom.
● BMKGENE yog ib tug neeg koom tes ntawm Oxford Nanopore, nrog dual platform RNA/DNA ntawv pov thawj.
● Muaj cov qauv tseem ceeb ntawm cov sequencers nrog cov cuab yeej ua tiav thiab cov khoom sib txuas txaus.
● Raws li Nanopore platform, ntau tshaj 10 tsiaj thiab tsob nroj Denovo tshawb fawb tau luam tawm nyob rau hauv thoob ntiaj teb cov ntaub ntawv renowned.

Cov Qauv Xav Tau

Hom qauv

Tus nqi

Concentrtion (Qubit ®)

Ntim

Purity

Lwm tus

Genomic DNA

Nyob ntawm cov ntaub ntawv xav tau

≥ 20ng / μl

≥15μl

OD260/280 = 1.7-2.2;

OD 260/230 ≥1.5;

Clear ncov ntawm 260 nm, tsis muaj kab mob

Concentration yuav tsum tau ntsuas los ntawm Qubit thiab Qubit/Nanopore ≤ 2

Total RNA

≥1.2 μg

≥100μg / μl

≥15μl

OD260/280 = 1.7-2.5;

OD260/230 = 0.5-2.5; Tsis muaj kab mob

RIN tus nqi ≥7.5

Kev Pabcuam Ua Haujlwm

Kev npaj ua qauv

Kev tsim kho tsev qiv ntawv

Sequencing

Cov ntaub ntawv tsom xam

Qhov project xa

Yav dhau los: DNA / RNA Sequencing - Illumina Sequencer

Tom ntej: Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Kev soj ntsuam zoo ntawm cov ntaub ntawv DNA Sample

Table 1. Kev txheeb xyuas ntawm cov ntaub ntawv huv.

BMKID	rawSqNum	rawSumBase	huvSeqNum	huvSumBase	huv N50Len	huv N90Len	cleanMeanLen	huv MaxLen	cleanMeanQual
DNA_BMK01	1, 218, 239 ib	26.37 Nws	1, 121, 736 ib	25.90 Nws	28.014 Nws	15,764 ib	23.090 Nws	143, 181 ib	9