BMKCloud Nkag mus

Cov khoom

Kev Tshawb Xyuas DNA/RNA - Nanopore Sequencer

ONT sequencing yog ib lub tshuab hluav taws xob tib lub sijhawm raws li nanopores, lub hauv paus ntsiab lus ntawm txhua lub platform yog tib yam. Ob chav-stranded DNA / RNA yuav khi rau nanoporous protein embedded nyob rau hauv biofilm thiab unwinding nyob rau hauv lub cev muaj zog protein, nyob rau hauv qhov kev ua ntawm voltage sib txawv los ntawm ob sab ntawm biofilm, DNA / RNA strands dhau los ntawm nanopore channel protein ntawm ib qho nqi. Vim yog qhov sib txawv ntawm cov khoom siv tshuaj lom neeg ntawm cov hauv paus sib txawv ntawm DNA / RNA strand, thaum ib lub hauv paus lossis DNA molecule dhau los ntawm nanopore channel, nws yuav ua rau muaj kev hloov pauv ntawm cov teeb liab hluav taws xob sib txawv. Los ntawm kev ntes thiab sib raug rau cov teeb liab no, cov hom hauv paus sib xws tuaj yeem suav tau, thiab kev ntes lub sijhawm tiag tiag ntawm cov kab ke tuaj yeem ua tiav.

Tiv Tauj Peb

Cov Lus Qhia Txog Kev Pabcuam

Cov txiaj ntsig ntawm kev sim

Cov Lus Qhia Txog Kev Pabcuam Nta

Platform	Qhov Loj ntawm Tsev Qiv Ntawv	Cov Ntaub Ntawv Theoretical Yield (Ib Lub Cell)	Kev Ntsuas Ib Leeg	Cov ntawv thov
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Xov Tooj	85-92%	Kev hu xov tooj SV, De novo, Kev txheeb xyuas tag nrho, Iso-Seq, Kev piav qhia txog noob caj noob ces, Kev tshawb pom DNA methylation

Cov Kev Pabcuam Zoo

● Muaj ntau tshaj 5 xyoos kev paub txog PacBio sequencing platform nrog ntau txhiab qhov project kaw nrog ntau hom tsiaj.
● BMKGENE yog tus khub koom tes raug cai ntawm Oxford Nanopore, nrog rau daim ntawv pov thawj RNA/DNA ob lub platform.
● Muaj cov qauv tseem ceeb ntawm cov sequencers nrog cov khoom siv tiav thiab cov sequencing throughput txaus.
● Raws li Nanopore platform, ntau tshaj 10 cov tsiaj thiab cog Denovo kev tshawb fawb tau luam tawm hauv cov ntawv xov xwm thoob ntiaj teb.

Cov Qauv Yuav Tsum Tau Ua

Hom Qauv

Tus nqi

Kev Sib Sau Ua Ke (Qubit ®)

Ntim

Kev dawb huv

Lwm tus

DNA genomic

Nyob ntawm seb cov ntaub ntawv xav tau dab tsi

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260 / 230≥1.5;

Lub ncov meej ntawm 260 nm, tsis muaj kev ua qias tuaj

Yuav tsum ntsuas qhov concentration los ntawm Qubit thiab Qubit/Nanopore ≤ 2

Tag Nrho RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5; tsis muaj kev kis kab mob

Tus nqi RIN ≥7.5

Kev Ua Haujlwm Pabcuam

Kev npaj qauv

Kev tsim kho tsev qiv ntawv

Kev txheeb xyuas kab ke

Kev tshuaj xyuas cov ntaub ntawv

Kev xa khoom ntawm qhov project

Yav dhau los: Cov tsev qiv ntawv Illumina uas tau ua tiav lawm

Tom ntej no: Kev Tshawb Fawb Txog Qhov Tshwj Xeeb (Specific-Locus Amplified Fragment Sequencing) (SLAF-Seq)

Kev Ntsuam Xyuas Cov Ntaub Ntawv Zoo ntawm Cov Qauv DNA

Rooj 1. Cov ntaub ntawv txheeb cais ntawm cov ntaub ntawv huv.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	huvN50Len	huv N90Len	huv siMeanLen	cleanMaxLen	huv si
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9

Kev Ntsuam Xyuas Cov Ntaub Ntawv Zoo ntawm RNA Qauv

Rooj 1. Cov ntaub ntawv txheeb cais ntawm cov ntaub ntawv huv.

Lub Npe Ntaub Ntawv	Tus Neeg Siv Khoom ID	Nyeem Tus lej	Tus lej hauv paus	N50	Nruab Nrab Ntev	Qhov Ntev Tshaj Plaws	Qhov nruab nrab Qscore
RNA_BMK001	C2	8,947,708	4,047,230,083	398	452	129,227	Q12