BMKCloud Nkag mus

Cov khoom

Cov tsev qiv ntawv DNBSEQ uas tau ua tiav lawm

DNBSEQ, tsim los ntawm MGI, yog ib qho thev naus laus zis NGS tshiab uas tau tswj kom txo qis tus nqi sequencing thiab ua kom muaj txiaj ntsig ntau dua. Kev npaj cov tsev qiv ntawv DNBSEQ suav nrog DNA fragmentation, kev npaj ssDNA, thiab dov lub voj voog amplification kom tau txais DNA nanoballs (DNB). Cov no tom qab ntawd raug thauj mus rau ntawm qhov chaw khov kho thiab tom qab ntawd sequenced los ntawm combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ thev naus laus zis sib xyaw ua ke cov txiaj ntsig ntawm kev muaj qhov yuam kev amplification qis nrog kev siv cov qauv yuam kev ceev ceev nrog nanoballs, ua rau sequencing nrog throughput siab dua thiab raug.

Peb qhov kev pabcuam sequencing uas tau ua tiav lawm ua rau cov neeg siv khoom tuaj yeem npaj Illumina sequencing libraries los ntawm ntau qhov chaw sib txawv (mRNA, tag nrho genome, amplicon, 10x libraries, thiab lwm yam), uas tau hloov mus rau MGI libraries hauv peb lub chaw soj nstuam kom raug sequenced hauv DNBSEQ-T7, ua rau muaj cov ntaub ntawv ntau ntawm cov nqi qis dua.

Tiv Tauj Peb

Cov Lus Qhia Txog Kev Pabcuam

Cov txiaj ntsig ntawm kev sim

Cov yam ntxwv

●Platform:MGI-DNBSEQ-T7

●Cov hom kev txheeb xyuas:PE150

●Kev hloov pauv ntawm Illumina cov tsev qiv ntawv mus rauMGI:ua kom muaj kev txheeb xyuas cov ntaub ntawv ntau ntawm tus nqi qis.

●Kev tswj xyuas zoo ntawm cov tsev qiv ntawv ua ntej kev txheeb xyuas cov ntaub ntawv.

●Kev tshuaj xyuas cov ntaub ntawv sequencing QC thiab kev xa khoom:Kev xa daim ntawv qhia QC thiab cov ntaub ntawv raw hauv hom ntawv fastq tom qab demultiplexing thiab lim cov ntawv nyeem Q30.

Cov txiaj ntsig

●Kev ua tau ntau yam ntawm cov kev pabcuam Sequencing:tus neeg siv khoom xaiv tau los ua qhov kev txiav txim siab raws li txoj kab lossis qhov ntau ntawm cov ntaub ntawv.

●Cov ntaub ntawv tso zis siab:1500 Gb/txoj kab

●Kev xa daim ntawv qhia txog kev txheeb xyuas QC:nrog rau cov ntsuas zoo, qhov tseeb ntawm cov ntaub ntawv thiab kev ua tau zoo tag nrho ntawm qhov project sequencing.

●Cov txheej txheem txheeb xyuas cov neeg laus:nrog lub sijhawm luv luv tig rov qab.

●Kev Tswj Xyuas Zoo: peb siv cov kev cai QC nruj heev kom lav qhov xa khoom zoo tas li.

Cov Qauv Yuav Tsum Tau Ua

	Tus Nqi Ntaub Ntawv (X)	Kev Ntsuas (qPCR/nM)	Ntim
Txoj Kev Ib Nrab	X ≤ 10 Gb	≥ 1nM	≥ 25 μl
	10 Gb < X ≤ 50 Gb	≥ 2 nM	≥ 25 μl
	50 Gb < X ≤ 100 Gb	≥ 3 nM	≥ 25 μl
	X > 100 Gb	≥ 4 nM
Txoj Kev Ib Leeg	Ib Txoj Kab	≥ 1.5 nM / Pas dej ua ke hauv tsev qiv ntawv	≥ 25 μl / pas dej hauv tsev qiv ntawv

Ntxiv rau qhov concentration thiab tag nrho cov nyiaj, kuj xav tau tus qauv ncov tsim nyog.

Lus Cim: Kev txheeb xyuas txoj kab ntawm cov tsev qiv ntawv tsis muaj ntau haiv neeg xav tau PhiX spike-in kom ntseeg tau tias muaj kev hu xov tooj ruaj khov.

Peb xav kom xa cov tsev qiv ntawv uas tau muab tso ua ke ua qauv. Yog tias koj xav tau BMKGENE los ua cov tsev qiv ntawv sib sau ua ke, thov mus saib

cov kev cai ntawm tsev qiv ntawv rau kev txheeb xyuas ib nrab txoj kab.

Qhov Loj ntawm Tsev Qiv Ntawv (Daim Ntawv Qhia Qhov Siab Tshaj Plaws)

Lub ncov tseem ceeb yuav tsum nyob hauv 300-450 bp.

Cov tsev qiv ntawv yuav tsum muaj ib lub ncov tseem ceeb, tsis muaj kev sib kis ntawm lub adapter thiab tsis muaj cov primer dimers.

Kev Ua Haujlwm Pabcuam

Yav dhau los: Kev Sib Cuam Tshuam ntawm Chromatin raws li Hi-C

Tom ntej no: BMKMANU S3000_Spatial Transcriptome

Daim ntawv qhia txog QC ntawm tsev qiv ntawv

Muaj ib daim ntawv qhia txog qhov zoo ntawm lub tsev qiv ntawv ua ntej kev txheeb xyuas, kev ntsuas tus nqi ntawm lub tsev qiv ntawv, thiab kev faib ua feem.

Daim ntawv qhia txog kev txheeb xyuas QC

Rooj 1. Cov ntaub ntawv txheeb cais ntawm cov ntaub ntawv sequencing.

Tus qauv ID	BMKID	Cov ntawv nyeem tsis tau ua tiav	Cov Ntaub Ntawv Raw (bp)	Nyeem huv si (%)	Q20 (%)	Q30 (%)	GC (%)
C_01	BMK_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	BMK_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08