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DNBSEQ cov tsev qiv ntawv ua ntej

DNBSEQ pre-made cov tsev qiv ntawv Featured duab

DNBSEQ, tsim los ntawm MGI, yog ib qho tshiab NGS thev naus laus zis uas tau tswj kom txo qis cov nqi sib txuas ntxiv thiab nce kev nce qib. Kev npaj ntawm DNBSEQ cov tsev qiv ntawv suav nrog DNA fragmentation, kev npaj ntawm ssDNA, thiab dov lub voj voog kom tau txais DNA nanoballs (DNB). Cov no yog tom qab ntawd thauj mus rau ib qho khoom nto thiab tom qab sib txuas los ntawm combinatorial Probe-Anchor Synthesis (cPAS). DNBSEQ thev naus laus zis sib xyaw ua ke qhov zoo ntawm qhov muaj qhov tsis zoo ntawm kev ua yuam kev tsawg nrog kev siv cov qauv kev ua yuam kev siab nrog nanoballs, uas ua rau muaj kev sib txuas nrog ntau dua thiab qhov tseeb.

Peb lub tsev qiv ntawv ua ntej ua tiav cov kev pabcuam ua rau cov neeg siv khoom npaj Illumina sequencing cov tsev qiv ntawv los ntawm ntau qhov chaw (mRNA, tag nrho cov genome, amplicon, 10x cov tsev qiv ntawv, thiab lwm yam), uas tau hloov mus rau MGI cov tsev qiv ntawv hauv peb lub chaw soj nstuam kom ua tiav hauv DNBSEQ-T7, ua kom cov ntaub ntawv siab ntawm tus nqi qis.

Tiv tauj peb

Kev Pabcuam Paub meej

Kev tshwm sim Demo

Nta

●Platform:MGI-DNBSEQ-T7

●Sequence hom:PEB 150

●Hloov ntawm Illumina cov tsev qiv ntawv mus rauMGI:enabling sequencing ntawm siab cov ntaub ntawv ntim ntawm tus nqi qis.

●Kev tswj xyuas cov tsev qiv ntawv zoo ua ntej ua ntu zus.

●Sequencing cov ntaub ntawv QC thiab xa:xa ntawm QC daim ntawv qhia thiab cov ntaub ntawv nyoos hauv fastq hom tom qab demultiplexing thiab lim Q30 nyeem.

Qhov zoo

●Versatility ntawm Sequence Services:tus neeg siv khoom tuaj yeem xaiv ua ntu ntawm txoj kab lossis tus lej ntawm cov ntaub ntawv.

●High data output:1500 Gb / kab

●Kev xa khoom ntawm QC daim ntawv qhia:nrog rau kev ntsuas zoo, cov ntaub ntawv raug thiab tag nrho cov kev ua tau zoo ntawm qhov kev sib txuas ua ke.

●Mature sequencing txheej txheem:nrog lub sijhawm luv luv.

●Rigorous Quality Control: Peb ua raws li QC nruj me ntsis kom lav qhov kev xa khoom ntawm cov txiaj ntsig zoo.

Cov Qauv Xav Tau

	Tus nqi ntawm cov ntaub ntawv (X)	Concentration (qPCR/nM)	Ntim
Ib nrab Txoj Kev	X ≤ 10 Gb	≥ 1nM	≥ 25 μl
	10 Gb < X ≤ 50 Gb	≥ 2 nM	≥ 25 μl
	50 Gb < X ≤ 100 Gb	≥ 3 nM	≥ 25 μl
	X> 100 Gb	≥ 4 nM
Ib Txoj Kev	Ib Txoj Kev	≥ 1.5 nM / Lub tsev qiv ntawv	≥ 25 μl / Lub tsev qiv ntawv

Ntxiv nrog rau qhov concentration thiab tag nrho cov nyiaj, tus qauv tsim nyog ncov kuj yuav tsum tau.

Lus Cim: Txoj kab kev sib txuas ntawm cov tsev qiv ntawv muaj ntau haiv neeg tsawg yuav tsum tau PhiX spike-in los xyuas kom muaj kev hu xov tooj zoo.

Peb pom zoo kom xa cov tsev qiv ntawv pre-pooled ua qauv. Yog tias koj xav tau BMKGENE los ua cov tsev qiv ntawv sib koom ua ke, thov xa mus rau qhov

lub tsev qiv ntawv yuav tsum tau rau ib nrab txoj kab sequencing.

Library Size (Peak map)

Lub ncov loj yuav tsum nyob rau hauv 300-450 bp.

Cov tsev qiv ntawv yuav tsum muaj tib lub ncov loj, tsis muaj kab mob sib kis thiab tsis muaj primer dimers.

Kev Pabcuam Ua Haujlwm

Yav dhau los: Hi-C raws Chromatin Interaction

Tom ntej: BMKMANU S3000_Spatial Transcriptome

QC daim ntawv qhia

Ib daim ntawv qhia txog qhov zoo ntawm lub tsev qiv ntawv yog muab ua ntej sequencing, ntsuam xyuas cov tsev qiv ntawv, thiab fragmentation.

Sequencing QC daim ntawv qhia

Table 1. Kev txheeb cais ntawm cov ntaub ntawv sib txuas.

Qauv ID	BMKID	Raw nyeem	Cov ntaub ntawv raw (bp)	Kev nyeem ntawv huv (%)	Q20 (%)	Q30 (%)	GC(%)
C_01	BMK_01	22, 870, 120	6,861,036,000 ib	96.48 ib	99.14 ib	94.85 ib	36.67 ib
C_02	BMK_02	14, 717, 867 ib	4, 415, 360, 100	96.00 Nws	98.95 ib	93.89 ib	37.08 Nws