Evolutionary noob caj noob ces
Kev Pabcuam Zoo
Takagi et al.,Phau ntawv cog lus, xyoo 2013
●Comprehensive bioinformatic tsom xam:Ua kom muaj kev kwv yees ntawm kev sib txawv ntawm cov noob caj noob ces, uas qhia txog qhov muaj peev xwm hloov pauv ntawm cov tsiaj, thiab nthuav tawm kev sib raug zoo ntawm phylogenetic kev sib raug zoo ntawm cov tsiaj nrog txo qis kev cuam tshuam ntawm convergent evolution thiab parallel evolution.
●Optional customized tsom xam: xws li kev kwv yees ntawm lub sijhawm sib txawv thiab nrawm raws li qhov hloov pauv ntawm nucleotide thiab amino acids qib.
●Cov kws tshaj lij dav dav thiab cov ntaub ntawv tshaj tawm: BMKGene tau sau cov kev paub loj hauv cov pej xeem thiab cov kev hloov pauv noob caj noob ces rau ntau tshaj 15 xyoo, npog ntau txhiab hom, thiab lwm yam thiab tau pab txhawb rau ntau tshaj 1000 cov haujlwm siab tshaj tawm hauv Nature Communications, Molecular Plants, Plant Biotechnology Journal, thiab lwm yam.
● Pab pawg neeg paub txog bioinformatics thiab tshawb xyuas luv luv: Nrog kev paub zoo hauv kev tshuaj ntsuam genomics siab heev, BMKGene pab pawg xa cov kev tshuaj ntsuam xyuas nrog lub sijhawm nrawm nrawm.
● Kev them nyiaj yug tom qab muag:Peb qhov kev cog lus txuas ntxiv dhau qhov kev ua tiav nrog rau 3 lub hlis tom qab kev muag khoom. Nyob rau lub sijhawm no, peb muab cov phiaj xwm taug qab, kev pab daws teeb meem, thiab Q&A ntu los daws cov lus nug ntsig txog cov txiaj ntsig.
Service Specifications thiab cov kev xav tau
| Hom kev sequencing | Pom zoo ntsuas cov pej xeem | Sequencing tswv yim | Nucleotide xav tau |
| Tag nrho Genome Sequencing | ≥ 30 tus neeg, nrog ≥ 10 tus neeg los ntawm txhua pab pawg
| 10x ib | Concentration: ≥ 1 ng / µL Tag nrho cov nyiaj ≥ 30ng Txwv los yog tsis muaj degradation los yog paug |
| Specific-Locus Amplified Fragment (SLAF) | Tag qhov tob: 10x ib Cim npe: <400 Mb: WGS tau pom zoo <1Gb: 100K tags 1gb ib > 2Gb: 300K tags Max 500k tag | Cov ntsiab lus ≥ 5 ng / µL Tag nrho cov nyiaj ≥ 80 ng Nanodrop OD 260/280 = 1.6-2.5 Agarose gel: tsis muaj los yog txwv tsis pub degradation los yog paug
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Kev Pabcuam Ua Haujlwm Flow
Kev sim tsim
Qauv xa tuaj
Kev tsim kho tsev qiv ntawv
Sequencing
Cov ntaub ntawv tsom xam
Kev pab cuam tom qab muag
Kev pabcuam suav nrog kev txheeb xyuas cov qauv pejxeem (phylogenetic tree, PCA, pejxeem stratification chart), pejxeem ntau haiv neeg, thiab kev xaiv pejxeem (kev sib txuas tsis sib xws, xaiv cheb-xaiv ntawm qhov chaw zoo). Cov kev pabcuam tseem tuaj yeem suav nrog kev tshuaj ntsuam xyuas (xws li lub sijhawm sib txawv, cov noob ntws).
*Cov txiaj ntsig tau pom ntawm no yog tag nrho los ntawm genomes luam tawm nrog BMKGENE
1.Evolution tsom xam muaj kev tsim kho ntawm tsob ntoo phylogenetic, pej xeem cov qauv thiab PCA raws li kev hloov pauv caj ces.
Phylogenetic ntoo sawv cev rau taxonomic thiab evolutionary kev sib raug zoo ntawm cov tsiaj nrog cov poj koob yawm txwv.
PCA lub hom phiaj kom pom kev sib ze ntawm cov neeg nyob sab nraud.
Cov qauv pej xeem qhia pom muaj qhov sib txawv ntawm cov noob caj noob ces nyob rau hauv cov nqe lus ntawm allele frequencies.
Chen, thiab. al.,PNAS, xyoo 2020
2.Selective sweep
Kev xaiv cheb yog hais txog cov txheej txheem los ntawm qhov chaw zoo yog xaiv thiab zaus ntawm qhov chaw nruab nrab txuas ntxiv tau nce thiab cov chaw tsis sib txuas tau txo qis, ua rau txo qis hauv cheeb tsam.
Kev tshawb nrhiav genome-wide ntawm cov cheeb tsam xaiv cheb yog ua tiav los ntawm kev suav cov pej xeem genetic index (π, Fst, Tajima's D) ntawm tag nrho SNPs nyob rau hauv lub qhov rais swb (100 Kb) ntawm qee cov kauj ruam (10 Kb).
Nucleotide diversity (π)
Tajima's D
Fixation index (Fst)
Wu, ib. al.,Molecular Cog, xyoo 2018
3.Gene Flow
Wu, ib. al.,Molecular Cog, xyoo 2018
4.Demographic keeb kwm
Zhang, thiab. al.,Nature Ecology & Evolution, 2021 ib
5. Lub sijhawm sib txawv
Zhang, thiab. al.,Nature Ecology & Evolution, 2021 ib
Tshawb nrhiav cov kev nce qib uas tau pab txhawb los ntawm BMKGene cov kev pabcuam evolutionary noob caj noob ces los ntawm kev sau cov ntawv tshaj tawm:
Hassanyar, AK et al. (2023) 'Discovery of SNP Molecular Markers thiab Candidate Genes Associated with Sacbrood Virus Resistance in Apis cerana cerana Larvae by Whole-Genome Resequencing',International Journal of Molecular Sciences, 24(7). doi: 10.3390/IJMS24076238.
Chai, J. et al. (2022) 'Nrhiav cov tsiaj qus, noob caj noob ces Suav loj heev salamander tsim kev txuag tshiab',Kev tshawb fawb Zoological, 2022, Peb. 43, Nqe 3, Nplooj 469-480, 43(3), pp. 469–480. doi: 10.24272/J.ISSN.2095-8137.2022.101.
Han, M. et al. (2022) 'Phylogeographical Pattern and Population Evolution History of Indigenous Elymus sibiricus L. on Qinghai-Tibetan Plateau',Frontiers hauv Plant Science, 13, ib. 882601. doi: 10.3389/FPLS.2022.882601/BIBTEX.
Wang, J. et al. (2022) 'Genomic insights into longan evolution from a chromosome-theem genome assembly thiab pejxeem genomics of longan accessions',Kev Tshawb Fawb Txog Kev Tshawb Fawb, 9. doi: 10.1093/HR/UHAC021.
