Kev Hloov Pauv Genes
Cov Kev Pabcuam Zoo
Takagi thiab lwm tus,Phau ntawv xov xwm ntawm cov nroj tsuag, 2013
●Kev tshuaj xyuas bioinformatic kom ntxaws:ua kom muaj peev xwm kwv yees txog kev sib txawv ntawm cov noob caj noob ces, uas qhia txog lub peev xwm ntawm kev hloov pauv ntawm cov tsiaj txhu, thiab qhia txog kev sib raug zoo ntawm cov tsiaj txhu nrog kev cuam tshuam tsawg kawg ntawm kev hloov pauv sib xyaw thiab kev hloov pauv sib luag.
●Kev tshuaj xyuas raws li qhov xav tau: xws li kev kwv yees lub sijhawm sib txawv thiab qhov ceev raws li kev hloov pauv ntawm nucleotide thiab amino acids theem.
●Kev Paub Ntau thiab Cov Ntaub Ntawv Tshaj TawmBMKGene tau muaj kev paub dhau los ntau heev hauv cov haujlwm txog pej xeem thiab kev hloov pauv noob caj noob ces rau ntau tshaj 15 xyoo, suav nrog ntau txhiab hom tsiaj, thiab lwm yam thiab tau pab txhawb rau ntau tshaj 1000 qhov haujlwm siab tshaj tawm hauv Nature Communications, Molecular Plants, Plant Biotechnology Journal, thiab lwm yam.
● Pab neeg bioinformatics txawj ntse heev thiab lub voj voog tshuaj xyuas luv luv: nrog kev paub zoo hauv kev tshuaj xyuas genomics siab heev, pab neeg BMKGene xa cov kev tshuaj xyuas tiav nrog lub sijhawm tig rov qab sai.
● Kev Txhawb Nqa Tom Qab Muag Khoom:Peb txoj kev cog lus tseem ceeb tshaj qhov ua tiav qhov project nrog rau 3 lub hlis kev pabcuam tom qab muag. Thaum lub sijhawm no, peb muab kev saib xyuas project, kev pab daws teeb meem, thiab cov lus nug thiab lus teb los teb cov lus nug ntsig txog cov txiaj ntsig.
Cov Lus Qhia Txog Kev Pabcuam thiab Cov Kev Cai
| Hom kev txheeb xyuas | Cov pejxeem pom zoo | Txoj kev npaj ua sequencing | Cov kev xav tau ntawm nucleotide |
| Kev Tshawb Fawb Txog Tag Nrho Genome | ≥ 30 tus neeg, nrog ≥ 10 tus neeg los ntawm txhua pawg me
| 10x | Kev Ntsuas: ≥ 1 ng/ µL Tag nrho cov nyiaj ≥ 30ng Txwv los yog tsis muaj kev puas tsuaj lossis kev ua qias tuaj |
| Kev Sib Txuas Lus Tshwj Xeeb (SLAF) | Qhov tob ntawm daim ntawv cim npe: 10x Tus naj npawb ntawm cov cim npe: <400 Mb: WGS raug pom zoo <1Gb: 100K daim ntawv cim npe 1Gb >2Gb: 300K daim ntawv cim npe Cov cim npe siab tshaj 500k | Kev Ntsuas ≥ 5 ng/µL Tag nrho cov nyiaj ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Agarose gel: tsis muaj lossis tsawg dua kev puas tsuaj lossis kev ua qias tuaj
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Kev Ua Haujlwm Pabcuam
Tsim qauv kev sim
Kev xa khoom qauv
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Cov kev pab cuam tom qab muag
Kev pabcuam suav nrog kev tshuaj xyuas cov qauv ntawm cov pejxeem (tsob ntoo phylogenetic, PCA, daim ntawv qhia txog cov pejxeem), kev sib txawv ntawm cov pejxeem, thiab kev xaiv cov pejxeem (kev sib txuas tsis sib npaug, kev xaiv cov chaw zoo). Kev pabcuam kuj tseem tuaj yeem suav nrog kev tshuaj xyuas raws li tus kheej (piv txwv li lub sijhawm sib txawv, kev ntws ntawm cov noob caj noob ces).
*Cov txiaj ntsig ntawm kev sim qhia ntawm no yog los ntawm cov genomes luam tawm nrog BMKGENE
1. Kev tshuaj xyuas kev hloov pauv muaj xws li kev tsim cov ntoo phylogenetic, cov qauv pej xeem thiab PCA raws li kev hloov pauv caj ces.
Tsob ntoo phylogenetic sawv cev rau kev sib raug zoo ntawm taxonomic thiab evolutionary ntawm cov tsiaj nrog cov poj koob yawm txwv.
PCA lub hom phiaj yog los pom kev sib ze ntawm cov pej xeem me.
Cov qauv pej xeem qhia tau tias muaj cov pej xeem sib txawv ntawm cov noob caj noob ces hauv cov nqe lus ntawm cov zaus allele.
Chen, thiab lwm tus,PNAS, 2020
2. Xaiv kev cheb
Kev xaiv tsa yog hais txog ib qho txheej txheem uas xaiv qhov chaw zoo thiab cov zaus ntawm cov chaw tsis txuas nrog tau nce ntxiv thiab cov chaw tsis txuas tau txo qis, ua rau txo qis ntawm thaj tsam.
Kev tshawb pom thoob plaws genome ntawm cov cheeb tsam xaiv tau ua tiav los ntawm kev xam cov pej xeem cov noob caj noob ces （π, Fst, Tajima's D) ntawm txhua SNPs hauv lub qhov rais swb (100 Kb) ntawm qee kauj ruam (10 Kb).
Kev sib txawv ntawm cov nucleotide (π)
Tajima's D
Kev kho qhov ntsuas (Fst)
Wu, thiab lwm tus,Tsob nroj molecular, 2018
3. Kev Hloov Pauv ntawm Cov Gene
Wu, thiab lwm tus,Tsob nroj molecular, 2018
4. Keeb kwm ntawm cov pej xeem
Zhang, thiab lwm tus,Kev Kawm Txog Xwm Txheej thiab Kev Hloov Pauv, 2021
5. Lub sijhawm sib txawv
Zhang, thiab lwm tus,Kev Kawm Txog Xwm Txheej thiab Kev Hloov Pauv, 2021
Tshawb nrhiav kev nce qib uas BMKGene cov kev pabcuam txog kev hloov pauv noob caj noob ces tau pab txhawb los ntawm kev sau cov ntawv tshaj tawm uas tau xaiv los ntawm:
Hassanyar, AK et al. (2023) 'Kev Tshawb Pom Cov Cim Molecular SNP thiab Cov Genes Candidate Associated with Sacbrood Virus Resistance in Apis cerana cerana Larvae by Whole-Genome Resequencing',Phau ntawv xov xwm thoob ntiaj teb ntawm kev tshawb fawb molecular, 24(7). doi: 10.3390/IJMS24076238.
Chai, J. et al. (2022) 'Kev tshawb pom ib tug tsiaj qus, ntshiab caj ces Suav teb loj salamander tsim cov cib fim tshiab rau kev txuag',Kev Tshawb Fawb Txog Tsiaj, 2022, Vol. 43, Issue 3, Nplooj: 469-480, 43(3), pp. 469–480. doi: 10.24272/J.ISSN.2095-8137.2022.101.
Han, M. et al. (2022) 'Phylogeographical Pattern and Population Evolution History of Indigenous Elymus sibiricus L. on Qinghai-Tibetan Plateau',Cov ciam teb hauv Kev Tshawb Fawb Txog Tsob Ntoo, 13, nplooj 882601. doi: 10.3389/FPLS.2022.882601/BIBTEX.
Wang, J. et al. (2022) 'Kev nkag siab txog genomic rau hauv kev hloov pauv ntawm longan los ntawm kev sib sau ua ke ntawm chromosome-level genome thiab cov pejxeem genomics ntawm longan accessions',Kev Tshawb Fawb Txog Kev Cog Qoob Loo, 9. doi: 10.1093/HR/UHAC021.
