BMKCloud Nkag mus

Cov khoom

Exosomal mRNA/LncRNA/CircRNA Sequencing-Illumina

Exosomes yog cov vesicles me me uas cov cell tso tawm, feem ntau muaj qhov loj li ntawm 30 txog 100 nanometers. Cov vesicles no muaj ntau yam RNA. Exosomes ntseeg tau tias ua lub luag haujlwm tseem ceeb hauv kev sib txuas lus ntawm cov cell, kev tiv thaiv kab mob, thiab kev loj hlob ntawm kab mob, thiab tuaj yeem kis mus rau lwm qhov ntawm lub cev los ntawm cov kua dej hauv lub cev xws li plasma, qaub ncaug, thiab zis. Lawv nqa cov biomolecules tshwj xeeb los tswj cov haujlwm ntawm cov cell txais, cuam tshuam rau cov xwm txheej cellular physiological. Exosomes kuj raug suav hais tias ua lub luag haujlwm tseem ceeb hauv kev loj hlob ntawm kab mob, suav nrog mob qog noj ntshav, kab mob neurodegenerative, thiab cov mob inflammatory. Kev tshawb fawb txog exosomes muab cov kev nkag siab tshiab thiab cov txheej txheem rau kev kuaj mob, kev kho mob, thiab kev tiv thaiv kab mob.

Tiv Tauj Peb

Cov Lus Qhia Txog Kev Pabcuam

Kev tshawb fawb txog bioinformatics

Cov Txiaj Ntsig Qhia Txog Kev Ua Qauv

Cov yam ntxwv

● Cov probes tshwj xeeb siv los ua kom cov ribosomal cDNA ploj mus.

● Kev tsim kho tsev qiv ntawv tshwj xeeb rau ntawm ntug dej.

● Ib txoj haujlwm ua haujlwm kom tau txais cov ntaub ntawv mRNA, lncRNA thiab circRNA tib lub sijhawm.

Cov Kev Pabcuam Zoo

● Kev Tswj Xyuas Zoo: Peb siv cov ntsiab lus tseem ceeb thoob plaws txhua theem, txij li kev npaj cov qauv thiab tsev qiv ntawv mus rau kev txheeb xyuas thiab bioinformatics. Qhov kev saib xyuas zoo no ua kom ntseeg tau tias muaj cov txiaj ntsig zoo tas li.

● Kev Pab Tom Qab Muag Khoom: Peb txoj kev cog lus txuas ntxiv mus dhau qhov project ua tiav nrog 3 lub hlis kev pabcuam tom qab muag khoom. Thaum lub sijhawm no, peb muab kev saib xyuas project, kev pab daws teeb meem, thiab cov lus nug thiab lus teb los teb cov lus nug ntsig txog cov txiaj ntsig.

Cov Qauv Yuav Tsum Tau Ua thiab Kev Xa Khoom

Tsev qiv ntawv	Platform	Cov ntaub ntawv pom zoo	Kev Tswj Xyuas Cov Ntaub Ntawv
rRNA depleted directional library	Illumina PE150	10-16 Gb	Q30≥85%

Cov Qauv Yuav Tsum Tau Ua

Cov nucleotides:

Tus nqi (ng)	Ntim (μL)
40	20

Cov ntaub so ntswg:

Exosome: 100 μL; 40 μg; >10^9 cov khoom me me/mL
Cov ntshav/ntshav: 5 mL
Cov tshuaj tua kab mob hauv lub hlwb: 100 mL
Cov zis: 100 mL
Cov qaub ncaug: 15 mL
Cov kua ntswg tawm: 15 mL
Cov kua dej hauv lub hlwb: 15 mL
Cov kua dej amniotic: 50 mL
Cov noob qes: 5 mL
Cov kua qaub: 5 ml
Ascites: 50 ml
Mis nyuj: 15 mL
Cov kua follicular: 15 mL
Cov kua txiv hmab txiv ntoo: 50 mL
Cov tshuaj tua kab mob hauv cov kab mob me me: 100 mL
Cov nqaij tsiaj: 3 g
Cov ntaub ntawm tsob nroj: 1 g

Kev Xa Qauv Pom Zoo

Lub thawv: 2 mL centrifuge tube (Tsis pom zoo kom siv ntawv ci)
Piv txwv kev sau npe: Pawg + theej piv txwv li A1, A2, A3; B1, B2, B3.
Kev Xa Khoom:
1. Dej khov qhuav: Cov qauv yuav tsum tau ntim rau hauv hnab thiab faus rau hauv dej khov qhuav.
2. Cov raj RNAstable: Cov qauv RNA tuaj yeem ziab hauv cov raj RNA stabilization (piv txwv li RNAstable®) thiab xa tuaj rau hauv chav tsev kub.

Kev Ua Haujlwm Pabcuam

Tsim qauv kev sim

Kev xa khoom qauv

Kev rho tawm RNA

Kev tsim kho tsev qiv ntawv

Kev txheeb xyuas kab ke

Kev tshuaj xyuas cov ntaub ntawv

Cov kev pab cuam tom qab muag

Yav dhau los: Kev Tshawb Fawb Txog RNA Me Me Exosomal-Illumina

Tom ntej no: [Luam] Eukaryotic mRNA Sequencing-NGS

Kev tshawb fawb txog bioinformatics

kev kwv yees circRNA: kev faib tawm ntawm chromosomal

Cov circRNAs Qhia Sib Txawv - Daim duab qhia txog roob hluav taws

Kev Qhia Txog CircRNAs Sib Txawv - Kev Sib Koom Tes Hauv Hierarchical

Kev txhim kho kev ua haujlwm ntawm circRNA cov noob caj noob ces

Kev tshuaj xyuas qhov sib txawv ntawm cov noob caj noob ces (DEGs)

Kev ntsuas ntawm lncRNA kev qhia tawm - kev sib sau ua ke

Kev nplua nuj ntawm cov noob caj noob ces lncRNA

Kev tshuaj xyuas qhov chaw sib koom ua ke ntawm mRNA thiab lncRNA - Circos plot (lub voj voog nruab nrab yog mRNA thiab sab hauv cicrlce yog lncRNA)