TGuide Smart Magnetic Plant DNA Kit
Tus lej Catalog / ntim khoom
| Cat. tsis muaj | ID | Tus naj npawb ntawm kev npaj |
| 4993548 (lub thawv) | DP607-DE | 48 |
Cov lus qhia xaiv cov tshuaj reagent rau TGuide S16
| Cov kua qaub nuclei | Hom Qauv | Lub Npe Khoom | Qhov loj ntawm cov khoom ntim (npaj ua ntej) Lub thawv/ phaj | Miv tsis muaj. Lub thawv/phaj |
| DNA | Cov nqaij tsiaj | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Cog thiab noob | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Av/Chaw | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| DNA | Cov khoom siv Gel & PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Ntshav Tag Nrho | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Qhuav pob, thiab lwm yam | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Ntshav/Cell/Nqaij | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Cog thiab noob | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Cov kua dej tsis muaj cell (Serum, thiab lwm yam) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Kev taw qhia txog khoom
Siv tau dav: Nws yog qhov tsim nyog rau ntau yam ntawm cov nroj tsuag cov ntaub so ntswg, tshwj xeeb tshaj yog polysaccharide- lossis polyphenol-nplua nuj nroj tsuag.
Muaj kev nyab xeeb thiab tsis muaj tshuaj lom: Tsis muaj cov tshuaj lom organic xws li phenol / chloroform.
Kev ntshiab siab: Cov DNA tau txais muaj kev ntshiab siab thiab tuaj yeem siv ncaj qha rau kev tshawb pom chip, kev ua tiav high-throughput thiab lwm yam kev sim.
Cov yam ntxwv
Siv tau yooj yim heev
Tsis muaj kev ua haujlwm pipetting ntxiv.
Qhov ntim elution tuaj yeem hloov kho raws li qhov xav tau.
Kev teeb tsa thiab kev siv tsis tas yuav kawm ntau. Yog tias koj muaj cov program uas twb muaj lawm, qhib lub cartridge, xaiv cov protocol thiab sim ua. Cov program no npaj siv tau, thiab kuj hloov kho tau raws li koj xav tau.
Cov tshuaj reagents uas tau muab tso ua ntej lawm thiab cov khoom siv pov tseg uas sib phim.
Kev siv tshuaj lom neeg raug tshem tawm kom zoo tshaj plaws kom ntseeg tau tias muaj kev nyab xeeb hauv kev ua haujlwm.
Cov txiaj ntsig ntawm kev sim
Kev rho tawm DNA genomic ntawm qos yaj ywm
Qhov loj ntawm cov qauv: 100 mg
Kev kho ua ntej qauv: sib tsoo nrog cov kua nitrogen lossis cov khoom siv homogenizer
Agarose gel concentration: 2% (TAE)
Ntim khoom: 2 μl
Cim: D15000, TIANGEN S16-01, S16-02 thiab S16-03 sawv cev rau kev sim ua ke ntawm kev rho tawm cov noob caj noob ces DNA los ntawm kev siv 3 TGuide S16 Nucleic Acid Extractors tib lub sijhawm.
Ib Lub Tuam Txhab: ib lub npe nrov
Cov txiaj ntsig ntawm kev sim: siv TGuide S16 nrog reagent 4993548 los rho tawm cov genomic DNA ntawm cov qos yaj ywm tau ua tiav cov txiaj ntsig zoo thiab muaj kev ntshiab siab, uas sib npaug rau cov khoom rho tawm zoo ntawm cov khoom sib tw spin-column extraction kits. Hauv qhov kev sim no, kwv yees li 11 μg ntawm nucleic acid tau rho tawm ntawm 100 mg qos yaj ywm buds, nrog OD260 / OD280 nyob ib puag ncig hauv 1.8 ~ 1.9, thiab OD260 / OD230 > 2.0.
Kev rho tawm DNA genomic ntawm nplooj taum pauv
Qhov loj ntawm cov qauv: 100 mg
Kev kho ua ntej qauv: sib tsoo nrog cov kua nitrogen lossis cov khoom siv homogenizer
Agarose gel concentration: 1% (TAE)
Ntim khoom: 2 μl
Cim: D15000, TIANGEN
Cov txiaj ntsig ntawm kev sim: siv TGuide S16 nrog reagent 4993548 los rho tawm cov genomic DNA ntawm nplooj taum pauv tau ua tiav qhov txiaj ntsig zoo thiab muaj kev ntshiab siab. Hauv qhov kev sim no, kwv yees li 13μg ntawm nucleic acid tau rho tawm ntawm 100 mg nplooj taum pauv, nrog OD260/OD280 nyob ib puag ncig 1.8 ~ 1.9, thiab OD260 / OD230 > 2.0.
Kev rho tawm cov noob qoob loo los ntawm cov noob caj noob ces (genomic DNA extraction)
Qhov loj ntawm cov qauv: 100 mg
Qauv ua ntej kev kho mob: sib tsoo nrog cov kua nitrogen lossis cov ntaub so ntswg sib tsoo homogenizer
Agarose gel concentration: 1% (TAE)
Ntim khoom: 2 μl
Cim: D15000, TIANGEN
Ib Lub Tuam Txhab: ib lub npe nrov
Cov txiaj ntsig ntawm kev sim: siv TGuide S16 nrog reagent 4993548 los rho tawm cov genomic DNA ntawm cov hauv paus nplej tau ua tiav cov txiaj ntsig zoo thiab kev ntshiab siab, uas sib npaug rau cov txiaj ntsig rho tawm thiab kev ntshiab ntawm cov neeg sib tw paub zoo. Ntxiv mus, kev ncaj ncees ntawm genomic DNA zoo dua li cov khoom sib tw ntawm lwm lub tuam txhab. Hauv qhov kev sim no, kwv yees li 15 μg ntawm nucleic acid tau rho tawm ntawm 100 mg cov hauv paus nplej, nrog OD260 / OD280 nyob ib puag ncig 1.8 ~ 1.9, thiab OD260 / OD230 > 2.0.
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