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Cov khoom

Cov kab mob fungal

Biomarker Technologies muab kev tshawb fawb genome, zoo genome thiab pene-ua tiav genome ntawm fungal nyob ntawm lub hom phiaj tshawb fawb tshwj xeeb.genome sequencing, sib dhos thiab kev ua haujlwm annotation tuaj yeem ua tiav los ntawm kev sib txuas ua ke Next-tiam sequencing + Peb tiam sequencing kom ua tiav qib siab genome los ua ke.Hi-C thev naus laus zis kuj tseem tuaj yeem ua haujlwm los pab txhawb genome los ua ke ntawm qib chromosome.

Platform:PacBio Sequel II

Nanopor PromethION P48

Illumina NovaSeq Platform


Cov ntsiab lus kev pabcuam

Cov txiaj ntsig Demo

Kev Pabcuam Zoo

● Ntau lub tswv yim sib txuas ua ke muaj rau cov hom phiaj tshawb fawb sib txawv

● Muaj kev paub dhau los hauv fungi genome los ua ke nrog ntau dua 10,000 pene-tiav genome assembled.

● Pab pawg kws tshaj lij tom qab muag kev pab txhawb nqa ua tiav cov kev tshawb fawb tshwj xeeb ntxiv.

Service Specifications

Kev pabcuam

Sequence Strategy

Zoo guaranteed

Kwv yees lub sijhawm tig rov qab

Fungal daim ntawv qhia zoo

Illumina 50X + Nanopore 100X

Contig N50≥2 Mb

35 hnub ua haujlwm

PacBio HiFi 30X

Fungal pene-ua tiav daim ntawv qhia

Illumina 50X + Nanopore 100X (Pacbio HiFi 30X) + Hi-C 100X

Chromosome anchoring ratio> 90%

45 hnub ua haujlwm

Bioinformatics tsom xam

● Raw cov ntaub ntawv zoo tswj

● Genome los ua ke

● Kev tshuaj ntsuam xyuas genome

● Gene muaj nuj nqi annotation

● Sib piv genomic tsom xam

2

Sample Requirements thiab Delivery

Cov Qauv Xav Tau:

RauDNA rho tawm:

Hom qauv

Tus nqi

Nco ntsoov

Purity

DNA rho tawm

1.2g 1

20 ng / μl

OD 260/280 = 1.6-2.5

Rau cov ntaub so ntswg:

Hom qauv Pom zoo coj mus kuaj Sample cia thiab xa khoom
Unicellular fungus Saib cov poov xab nyob rau hauv lub microscope thiab sau lawv nyob rau hauv lawv exponential theem

Hloov cov kab lis kev cai (muaj kwv yees li 3-4.5e9 hlwb) rau hauv 1.5 lossis 2 ml eppendorf.(Ceev rau dej khov)

Centrifuge lub raj rau 1 min ntawm 14000 g kom khaws cov kab mob thiab tshem tawm cov supernatant kom zoo

Kaw lub raj thiab khov cov kab mob hauv cov kua nitrogen rau tsawg kawg 1-3 teev.Khaws lub raj rau hauv -80 ℃ fridge.

Kho cov qauv hauv cov kua nitrogen rau 3-4 teev thiab khaws cia hauv cov kua nitrogen los yog -80 degree mus rau lub sijhawm ntev.Qauv shipping nrog qhuav-dej khov yog yuav tsum tau.
Macro fungus Cov ntaub so ntswg nyob rau theem nquag loj hlob yog pom zoo.

Yaug cov ntaub so ntswg nrog dej tsis muaj endotoxin, tom qab ntawd 70% ethonal.

Txuag cov qauv hauv cov raj cryo.

Kev Pabcuam Ua Haujlwm Flow

coj mus kuaj

Qauv xa tuaj

Kev npaj tsev qiv ntawv

Kev tsim kho tsev qiv ntawv

Sequencing

Sequencing

Cov ntaub ntawv tsom xam

Cov ntaub ntawv tsom xam

Kev pab cuam tom qab muag

Kev pab cuam tom qab muag


  • Yav dhau los:
  • Tom ntej:

  • 1.Circos daim duab ntawm fungal genomic Cheebtsam

    3

    2.Comparative genomics tsom xam: Phylogenetic tsob ntoo

    4

     

    tau ib nqe lus

    Sau koj cov lus ntawm no thiab xa tuaj rau peb

    Xa koj cov lus rau peb: