Kev Tshawb Fawb Txog Kev Koom Tes Thoob Plaws Genome
Kev Ua Haujlwm
Cov Kev Pabcuam Zoo
●Kev Paub Ntau thiab Cov Ntaub Ntawv Tshaj Tawm: nrog kev paub dhau los hauv GWAS, BMKGene tau ua tiav ntau pua qhov project txog hom tsiaj hauv kev tshawb fawb txog pej xeem GWAS, pab cov kws tshawb fawb tshaj tawm ntau dua 100 tsab xov xwm, thiab qhov cuam tshuam tag nrho tau txog 500.
● Kev tshuaj xyuas bioinformatics kom ntxaws: cov txheej txheem ua haujlwm suav nrog kev tshuaj xyuas kev sib raug zoo ntawm SNP, xa cov noob caj noob ces thiab lawv cov lus piav qhia ua haujlwm sib xws.
●Pab neeg bioinformatics txawj ntse thiab lub voj voog tshuaj xyuas luv luv: nrog kev paub zoo hauv kev tshuaj xyuas genomics siab heev, pab neeg BMKGene xa cov kev tshuaj xyuas tiav nrog lub sijhawm tig rov qab sai.
●Kev Txhawb Nqa Tom Qab Muag Khoom:Peb txoj kev cog lus tseem ceeb tshaj qhov ua tiav qhov project nrog rau 3 lub hlis kev pabcuam tom qab muag. Thaum lub sijhawm no, peb muab kev saib xyuas project, kev pab daws teeb meem, thiab cov lus nug thiab lus teb los teb cov lus nug ntsig txog cov txiaj ntsig.
Cov lus qhia thiab cov kev cai ntawm kev pabcuam
| Hom kev txheeb xyuas | Cov pejxeem pom zoo | Txoj kev npaj ua sequencing | Cov kev xav tau ntawm nucleotide |
| Kev Tshawb Fawb Txog Tag Nrho Genome | 200 qauv | 10x | Kev Ntsuas: ≥ 1 ng/ µL Tag nrho cov nyiaj ≥ 30ng Txwv los yog tsis muaj kev puas tsuaj lossis kev ua qias tuaj |
| Kev Sib Txuas Lus Tshwj Xeeb (SLAF) | Qhov tob ntawm daim ntawv lo: 10x Tus naj npawb ntawm cov cim npe: < 400 Mb: WGS raug pom zoo < 1Gb: 100K daim ntawv cim npe 1Gb > 2Gb: 300K daim ntawv cim npe Cov cim npe siab tshaj 500k | Kev Ntsuas ≥ 5 ng/µL Tag nrho cov nyiaj ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Agarose gel: tsis muaj lossis tsawg dua kev puas tsuaj lossis kev ua qias tuaj
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Kev Xaiv Khoom Siv
Cov ntau hom, cov subspecies, cov haiv neeg hauv av/cov genebanks/cov tsev neeg sib xyaw/cov peev txheej qus
Ntau hom, subspecies, landraces
Tsev neeg ib nrab kwv tij/tsev neeg tag nrho kwv tij/cov peev txheej qus
Kev Ua Haujlwm Pabcuam
Tsim qauv kev sim
Kev xa khoom qauv
Kev rho tawm RNA
Kev tsim kho tsev qiv ntawv
Kev txheeb xyuas kab ke
Kev tshuaj xyuas cov ntaub ntawv
Cov kev pab cuam tom qab muag
Muaj xws li cov kev tshuaj xyuas hauv qab no:
- Kev tshuaj xyuas kev sib koom ua ke thoob plaws genome: LM, LMM, EMMAX, FASTLMM qauv
- Kev piav qhia txog kev ua haujlwm ntawm cov noob caj noob ces
Kev tshuaj xyuas kev sib raug zoo ntawm SNP-trait - Manhattan plot
Kev tshuaj xyuas kev sib raug zoo ntawm SNP-txuj ci - QQ plot
Tshawb nrhiav cov kev nce qib uas BMKGene's de GWAS cov kev pabcuam tau pab txhawb los ntawm kev sau cov ntawv tshaj tawm uas tau xaiv los ntawm:
Lv, L. et al. (2023) 'Kev nkag siab txog lub hauv paus ntawm kev kam rau ammonia hauv razor clam Sinonovacula constricta los ntawm kev tshawb fawb txog kev sib raug zoo thoob plaws genome',Kev cog qoob loo hauv dej, 569, nplooj ntawv 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
Li, X. et al. (2022) 'Kev tshuaj xyuas ntau yam ntawm 398 cov noob foxtail millet qhia txog thaj chaw genomic cuam tshuam nrog kev yug tsiaj, cov yam ntxwv metabolite, thiab cov teebmeem tiv thaiv kev o',Tsob nroj molecular, 15(8), pp. 1367–1383. doi: 10.1016/j.molp.2022.07.003.
Li, J. et al. (2022) 'Genome-Wide Association Mapping of Hulless Barely Phenotypes in Drought Environment',Cov ciam teb hauv Kev Tshawb Fawb Txog Tsob Ntoo, 13, nplooj 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. et al. (2021) 'GmST1, uas encodes ib tug sulfotransferase, muab kev tiv thaiv rau soybean mosaic virus strains G2 thiab G3',Cog, Cell & Ib puag ncig, 44(8), pp. 2777–2792. doi: 10.1111/PCE.14066.
