Kev Sib Cuam Tshuam ntawm Chromatin raws li Hi-C
Cov yam ntxwv ntawm kev pabcuam
● Kev txheeb xyuas kab ke ntawm Illumina NovaSeq nrog PE150.
● Kev pabcuam yuav tsum tau cov qauv ntaub so ntswg, es tsis txhob siv cov kua qaub nucleic uas tau rho tawm, los txuas nrog formaldehyde thiab khaws cov kev sib cuam tshuam ntawm DNA-protein.
● Qhov kev sim Hi-C suav nrog kev txwv thiab kho qhov kawg ntawm cov nplaum nrog biotin, ua raws li kev sib hloov ntawm cov blunt kawg thaum khaws cov kev sib cuam tshuam. Cov DNA ces raug rub nrog streptavidin hlaws thiab purified rau kev npaj tsev qiv ntawv tom qab.
Cov Kev Pabcuam Zoo
●Kev Tsim Enzyme Zoo Tshaj Plaws: kom ntseeg tau tias muaj kev ua haujlwm siab Hi-C ntawm ntau hom tsiaj sib txawv nrog txog li 93% cov khub sib cuam tshuam.
●Kev Paub Ntau Thiab Cov Ntaub Ntawv Tshaj Tawm:BMKGene muaj kev paub dhau los ntau nrog >2000 Hi-C sequencing projects los ntawm 800 hom sib txawv thiab ntau yam patents. Tshaj 100 cov ntaub ntawv luam tawm nrog rau qhov cuam tshuam ntawm ntau dua 900.
●Pab Pawg Bioinformatics Muaj Kev Txawj Ntse:nrog cov ntawv pov thawj hauv tsev thiab cov cai software rau Hi-C kev sim thiab kev tshuaj xyuas cov ntaub ntawv thiab cov ntaub ntawv pom kev uas tus kheej tsim.
●Kev Txhawb Nqa Tom Qab Muag Khoom:Peb txoj kev cog lus tseem ceeb tshaj qhov ua tiav qhov project nrog rau 3 lub hlis kev pabcuam tom qab muag. Thaum lub sijhawm no, peb muab kev saib xyuas project, kev pab daws teeb meem, thiab cov lus nug thiab lus teb los teb cov lus nug ntsig txog cov txiaj ntsig.
●Cov Lus Qhia Txog Kev Ua Haujlwm: peb siv ntau lub ntaub ntawv los sau cov noob caj noob ces nrog cov kev hloov pauv uas tau txheeb xyuas thiab ua qhov kev tshuaj xyuas kev nplua nuj sib xws, muab kev nkag siab txog ntau qhov kev tshawb fawb.
Cov Lus Qhia Txog Kev Pabcuam
| Tsev qiv ntawv | Txoj Kev Npaj Txog Sequencing | Cov ntaub ntawv tso tawm pom zoo | Kev daws teeb meem Hi-C |
| Tsev qiv ntawv Hi-C | Illumina PE150 | Lub voj voog Chromatin: 150x TAB: 50x | Chromatin voj: 10Kb TAD: 40Kb |
Cov Kev Xav Tau Kev Pabcuam
| Hom qauv | Tus nqi yuav tsum tau muaj |
| Cov nqaij tsiaj | ≥2g |
| Ntshav Tag Nrho | ≥2mL |
| Cov nceb | ≥1g |
| Cov ntaub so ntswg hluas ntawm cov nroj tsuag | 1g/aliquot, pom zoo kom siv 2-4 aliquot |
| Cov Cell Uas Muaj Kab Mob | ≥1x107 |
Muaj xws li Kev Tshawb Fawb Hauv Qab No:
● Cov ntaub ntawv nyoos QC;
● Kev kos duab thiab Hi-C tsev qiv ntawv QC: cov khub sib cuam tshuam siv tau thiab Interaction Decay Exponents (IDEs);
● Kev Txheeb Xyuas Kev Sib Cuam Tshuam Thoob Plaws Genome: kev tshuaj xyuas cis/trans thiab daim ntawv qhia kev sib cuam tshuam Hi-C;
● Kev tshuaj xyuas ntawm kev faib tawm ntawm A/B compartment;
● Kev txheeb xyuas cov TADs thiab cov voj voog chromatin;
● Kev tshuaj xyuas sib txawv ntawm cov qauv chromatin 3D ntawm cov qauv piv txwv thiab cov lus piav qhia ua haujlwm ntawm cov noob caj noob ces cuam tshuam.
Kev faib tawm ntawm Cis thiab trans proportion
Daim ntawv qhia kub ntawm kev sib cuam tshuam ntawm cov chromosomal ntawm cov qauv kuaj
Kev faib tawm thoob plaws genome ntawm A / B compartments
Kev faib tawm thoob plaws genome ntawm chromatin loops
Kev Pom Cov TADs
Tshawb nrhiav kev nce qib ntawm kev tshawb fawb uas tau pab txhawb los ntawm BMKGene's Hi-C sequencing cov kev pabcuam los ntawm kev sau cov ntawv tshaj tawm.
Meng, T. et al. (2021) 'Kev tshuaj xyuas sib piv ntawm ntau yam kev sib xyaw ua ke qhia txog CA2 ua lub hom phiaj tshiab rau chordoma',Kev Kho Mob Cancer ntawm Lub Hlwb, 23(10), pp. 1709–1722. doi: 10.1093/NEUONC/NOAB156.
Xu, L. et al. (2021) '3D kev tsis sib haum xeeb thiab kev hloov pauv ntawm cov noob caj noob ces muab kev nkag siab txog pathogenesis ntawm NAFLD los ntawm kev sib xyaw ua ke Hi-C, Nanopore, thiab RNA sequencing',Acta Pharmaceutica Sinica B, 11(10), pp. 3150–3164. doi: 10.1016/J.APSB.2021.03.022.
