Kev Tshawb Fawb Txog Metabolomics
Cov yam ntxwv
●Tsis yogKev Tshawb Pom Metabolomics (Kev Tshawb Pom Metabolomics)
Raws li kev siv tshuab chromatography-mass spectrometry (LC-MS), cov txheej txheem metabolic uas tsis tsom mus rau qhov chaw ua rau muaj kev tshawb pom ntau yam metabolites me me hauv cov qauv kuaj mob xws li cov hlwb, cov nqaij, cov kabmob, lossis cov kua dej hauv lub cev. Nws ua qhov kev tshuaj xyuas sib piv ntawm cov pab pawg sim thiab cov pab pawg tswj, tshuaj xyuas cov metabolites sib txawv los ntawm kev tshuaj xyuas lej.
● Cov Metabolomics Tsis Yog Lub Hom Phiaj Rau Cov Lipids
Siv cov thev naus laus zis kua chromatography-mass spectrometry (LC-MS),nCov Metabolomics rau Lipids uas tau tsom mus rau ntawm lub hom phiaj ua tiav qhov kev tshawb pom tsis muaj kev ntxub ntxaug ntawm ntau cov lipid molecules li sai tau hauv cov qauv tshuaj lom neeg suav nrog cov hlwb, cov ntaub so ntswg, cov kabmob, lossis cov kua dej hauv lub cev.
● Dav-Kev Tshawb Fawb Txog Metabolomics
Qhov kev pabcuam no ua ke zoo kawg nkaus cov txiaj ntsig siab thiab dav dav ntawm cov thev naus laus zis tsis tsom mus rau qhov muaj kev nkag siab siab thiab muaj peev xwm ntsuas qhov tseeb ntawm cov thev naus laus zis MRM (Ntau Yam Kev Tshuaj Xyuas Kev Ntsuas). Cov txheej txheem ua haujlwm yog raws li nram no: Ua ntej, cov txheej txheem ntsuas qhov tseeb yog siv los ua qhov kev ntsuas MS² siab heev ntawm cov qauv kom tau txais MS² spectra ntawm "txhua" metabolites. Tom qab ntawd, kev txheeb xyuas metabolite raug ua los ntawm kev sib koom ua ke nrog lub tsev qiv ntawv MS² siab (npog ntau dua 25,000 metabolites, nrog ntau tus qauv siab daws teeb meem MS² spectra rau txhua metabolite). Tom ntej no, cov ntaub ntawv MRM ion khub raug rho tawm ntawm cov txheej txheem ntsuas qhov tseeb los tsim lub tsev qiv ntawv tshwj xeeb rau cov qauv. Thaum kawg, triple quadrupole mass spectrometry nrog MRM thev naus laus zis raug siv rau kev ntsuas qhov tseeb.
● Kev Kho Mob Metabolomics Uas Tau Targeted
Kev tshuaj xyuas cov tshuaj metabolimic tsom mus rau ntau yam tshuaj uas muaj lub hom phiaj lossis tag nrho/ib feem ntawm cov tshuaj metabolites uas koom nrog hauv ib txoj kev tshwj xeeb. Siv cov tshuaj txheem, nws tsim kom muaj ib txoj kev kuaj pom uas muaj qhov tshwj xeeb, muaj kev nkag siab zoo, thiab rov ua dua tau zoo rau kev ntsuas thiab kev tshuaj xyuas cov tshuaj uas muaj lub hom phiaj. Nws siv cov qauv sab nraud ua ke nrog cov qauv sab hauv rau kev ntsuas tag nrho, nrog rau qhov linearity ntawm cov kab nkhaus txheem ncav cuag ntau dua 0.99 thiab qhov rhiab heev txog li ng/mL.
Cov txiaj ntsig
●Lub Platform Tshawb Nrhiav Siab Tshaj Plaws
-Nruab nrog cov ntsuas qhov hnyav siab tshaj plaws thiab cov ntsuas qhov hnyav peb npaug plaub npaug, ua rau muaj peev xwm nrhiav tau thiab tshuaj xyuas ntau txhiab yam khoom noj khoom haus tib lub sijhawm.
●Cov Ntaub Ntawv Qhia Txog Kev Siv
- Cov Ntaub Ntawv Rau Pej Xeem: Siv rau METLIN, KEGG, HMDB, NP Atlas, thiab Lipidmaps, uas muaj ntau tshaj 500,000 metabolites.
- Hauv tsevCov Ntaub Ntawv Tshwj Xeeb rau Cov Nroj Tsuag: Muaj ntau dua 25,000 cov metabolites, suav nrog cov metabolites thawj zaug thiab cov metabolites thib ob.
- Hauv tsevCov Ntaub Ntawv Tshwj Xeeb rau Tsiaj/Kho Mob: Muaj ntau tshaj 12,000 cov metabolites.
●Kev Tswj Xyuas Zoo Nruj
-Kev tswj xyuas zoo nruj heev uas suav nrog kev ruaj khov ntawm cov cuab yeej, cov khoom seem, QC qauv PCA thiab kev tshuaj xyuas kev sib raug zoo kom ntseeg tau tias cov ntaub ntawv zoo.
●Muaj Peev Xwm Tshawb Pom Metabolite Siab
- Tsis yogKev Tshawb Fawb Txog Metabolomics: Nrhiav tau qhov nruab nrab ntawm ntau dua 4,200 metabolites ib zaug khiav, zoo tagnrho rau kev tshawb nrhiav cov metabolites tsis paub hauv cov qauv thiab txheeb xyuas cov metabolites hauv cov qauv nyuaj.
- Dav-Kev Tshawb Fawb Txog Metabolomics (Cog): Nrhiav tau qhov nruab nrab ntawm ntau dua 2,410 metabolites ib zaug khiav, suav nrog cov metabolites thawj zaug thiab theem ob.
- Dav-Kev Tshawb Fawb Txog Kev Ua Haujlwm (Tsiaj): Nrhiav tau qhov nruab nrab ntawm ntau dua 1,250 lub metabolites ib zaug khiav.
- Cov Metabolomics Uas Tau Targeted: Cov Vaj Huam Sib Luagrauntau pawg metabolite.
●Kev Tshawb Fawb Txog Kev Paub Tag Nrho
-Muaj ntau tshaj 10 yam khoom siv los tshuaj xyuas thiab ntau tshaj 20 daim duab qhia pom.
●Kev Pabcuam Tom Qab Muag Khoom Zoo
-Muab kev sab laj tom qab muag thiab kev txhawb nqa txhais lus rau daim ntawv qhia zaum kawg.
Cov Lus Qhia Txog Kev Pabcuam
| Kev daws teeb meem | Platform | Cov Kev Rov Ua Dua Tshiab Uas Pom Zoo |
|---|---|---|
| Cov Metabolomics Tsis Yog Lub Hom Phiaj | UHPLC-TOF-MS (Waters Xevo G2-XS QTof) | Cov qauv cog thiab cov kab mob me me: ≥ 6 Cov qauv tsiaj: ≥ 10 Cov qauv kuaj mob: ≥ 30 Tag nrho cov qauv kuaj kab mob uas rov ua dua tshiab tau soj ntsuam ywj pheej. |
| Kev Tshawb Fawb Txog Metabolomics Dav Dav | Dej Xevo G2-XS QTOF + AB Sciex QTRAP 6500+ | Cov qauv cog: ≥ 3 |
| Kev Tshawb Fawb Txog Metabolomics | UHPLC-QQQ-MS (AB Sciex QTRAP 6500+) GC-MS (Agilent 7890-5977, Agilent 7820-5977) | Cov qauv cog: ≥ 3 Cov qauv tsiaj: ≥ 6 |
Cov Qauv Yuav Tsum Tau Ua
Koj puas xav paub seb koj cov qauv puas ua tau raws li peb cov qauv? Nyem qhov no kom tau txais peb cov qauvcov qauv xav tau tshiab tshaj plaws.
Kev Ua Haujlwm Pabcuam
Cov Qauv Sau
Kev rho tawm cov tshuaj metabolite
Kev Tau Txais Cov Ntaub Ntawv
Kev Tshawb Fawb Txog Cov Ntaub Ntawv
Kev Xa Cov Ntaub Ntawv
1. Suab nrov tom qab thiab kev ua cov ntaub ntawv tsis zoo ntawm cov ntaub ntawv raw
2. Kev Ntsuam Xyuas Qhov Zoo ntawm Cov Ntaub Ntawv
2.1 Kev tshuaj xyuas cov khoom tseem ceeb
2.2 Kev ntsuam xyuas qhov rov ua dua
3. Cov Lus Cim Txog Metabolite
3.1 Cov lus piav qhia ntawm KEGG database
3.2 Cov lus piav qhia txog HMDB database
3.3 Cov lus piav qhia txog cov ntaub ntawv Lipidmaps
4. Kev Tshuaj Xyuas Cov Ntaub Ntawv Txog Kev Muab Pawg Piv Txwv (cov qauv tshuaj lom neeg rov ua dua)≥3)
4.1 Kev tshuaj xyuas cov khoom tseem ceeb ntawm pawg
4.2 Kev tshuaj xyuas qhov sib txawv ntawm cov duab plaub fab tsawg kawg nkaus (OPLS-DA)
4.3 Kev tshuaj xyuas cov metabolite sib txawv ntawm pawg
5. Kev Xaiv Metabolite Sib Txawv (kev rov ua dua ntawm cov kab mob hauv lub cev)≥3)
5.1 Kev tshuaj xyuas qhov sib txawv ntawm qhov quav
5.2 Daim duab qhia txog hluav taws kub sib txawv
5.3 Daim ntawv qhia kub ntawm cov metabolite clustering sib txawv
5.4 Daim duab qhia txog kev sib raug zoo ntawm cov metabolite sib txawv
5.5 Daim duab qhia txog cov metabolite Z-score sib txawv
5.6 Kev tshuaj xyuas cov duab radar sib txawv ntawm cov metabolite
5.7 Daim duab qhia txog kev sib txawv ntawm cov metabolite violin
5.8 Daim duab qhia txog cov khoom sib txawv ntawm cov metabolite box
5.9 Kev piav qhia txog kev ua haujlwm ntawm cov metabolite KEGG thiab kev txheeb xyuas kev nplua nuj
5.10 Kev tshuaj xyuas kab ROC
5.11 Kev sib txawv ntawm cov metabolite k-means clustering
5.12 Daim duab Venn ntawm cov metabolite sib txawv
1. Kev Ntsuam Xyuas Qhov Zoo ntawm Cov Ntaub Ntawv
↑Ckev tshuaj xyuas kev sib raug zoo(Cov qauv kuaj/QC qauv)
↑Kev tshuaj xyuas cov khoom tseem ceeb ↑Kev tshuaj xyuas cov Metabolite clustering
2. Cov Lus Cim Txog Metabolite
↑Cov lus piav qhia ntawm cov ntaub ntawv KEGG↑HMDB database annotation
↑Cov lus piav qhia ntawm cov ntaub ntawv Lipidmaps
3. Kev Tshuaj Xyuas Cov Ntaub Ntawv Txog Kev Muab Pawg Piv Txwv (cov qauv tshuaj lom neeg rov ua dua)≥3)
↑Kev tshuaj xyuas cov khoom tseem ceeb ntawm pawg(2D/3D)
↑Kev tshuaj xyuas discriminant partial least squares (OPLS-DA)
4. Kev Xaiv Metabolite Sib Txawv (kev rov ua dua ntawm cov kab mob hauv lub cev)≥3)
↑Kev tshuaj xyuas qhov hloov pauv ntawm qhov sib txawv↑Differential metabolite volcano plot
↑Differential metabolite clustering heatmap↑Differential metabolite correlation plot
↑Differential metabolite Z-score plot↑Kev tshuaj xyuas radar ntawm cov metabolite sib txawv
↑Differential metabolite violin plot↑Differential metabolite box plot
↑Differential metabolite KEGG kev ua haujlwm annotation thiab kev txheeb xyuas kev nplua nuj
↑Kev tshuaj xyuas kab nkhaus ROC↑Kev sib sau ua ke ntawm cov metabolite sib txawv k-means
↑Differential metabolite Venn daim duab
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