mRNA-seq (NGS) – De novo

mRNA sequencing tso cai rau profiling ntawm tag nrho cov mRNA transcripts hauv cell nyob rau hauv cov xwm txheej tshwj xeeb thiab yog ib qho thev naus laus zis siv dav hauv ntau qhov chaw tshawb fawb. Lub BMKCloud De novo mRNA-seq pipeline yog tsim los tshuaj xyuas poly-A enriched sequencing libraries thaum tsis muaj genome siv tau. Lub pipeline pib nrog kev tswj xyuas zoo, ua raws lide novokev sib dhos ua ke ntawm cov ntawv sau thiab kev xaiv cov teeb tsa unigene. Kev tshuaj xyuas cov qauv unigene kwv yees qhov kev ua lej coding (CDS) thiab cov kev ua lej yooj yim rov ua dua (SSR). Tom qab ntawd, kev tshuaj xyuas kev qhia sib txawv pom cov noob qhia sib txawv (DEGs) ntawm cov xwm txheej sim, ua raws li kev sau ntawv ua haujlwm thiab kev txhim kho ntawm DEGs kom rho tawm cov kev nkag siab txog kab mob.