Nanopore Cov ntawv sau ntev tag nrho

Nanopore transcriptome sequencing yog ib txoj kev muaj zog rau kev sequencing tag nrho-ntev cDNAs, txheeb xyuas thiab ntsuas cov transcript isoforms kom raug. Lub BMKCloud Nanopore Full-Length Transcriptome Pipeline yog tsim los tshuaj xyuas cov ntaub ntawv RNA-Seq tsim los ntawm Nanopore platform tawm tsam cov genome siv tau zoo, muab kev tshuaj xyuas zoo thiab ntau ntawm ob qho tib si gene thiab transcript theem. Tom qab kev tswj hwm zoo, cov kab ke tag nrho-ntev non-chimeric (FLNC) tau txais thiab cov kab ke sib koom siab tau mapped rau lub genome siv kom tshem tawm cov transcripts redundant. Los ntawm cov ntawv sau no, kev qhia tawm raug ntsuas thiab cov noob qhia tawm sib txawv thiab cov ntawv sau tau txheeb xyuas thiab ua haujlwm. Lub pipeline kuj suav nrog kev tshuaj xyuas lwm txoj hauv kev polyadenylation (APA), kev tshuaj xyuas lwm txoj hauv kev splicing, kev tshuaj xyuas yooj yim rov ua dua (SSR), kev kwv yees ntawm lncRNA thiab cov hom phiaj sib xws, kev kwv yees ntawm cov kab ke coding (CDS), kev tshuaj xyuas tsev neeg gene, kev tshuaj xyuas cov ntaub ntawv transcription, kev kwv yees ntawm cov noob tshiab thiab kev sau ntawv ua haujlwm ntawm cov ntawv sau.