Pacbio cov ntawv sau ntev tag nrho

PacBio Full-Length Transcriptome Sequencing, Isoseq, ua rau muaj kev txheeb xyuas qhov tseeb ntawm cov transcript isoforms, qhia txog lwm txoj hauv kev polyadenylation thiab splicing, thiab ua rau muaj kev txheeb xyuas qhov tseeb dua ntawm cov noob caj noob ces. Lub BMKCloud PacBio Full-Length Transcriptome pipeline yog tsim los tshuaj xyuas cov tsev qiv ntawv cDNA uas tau teeb tsa hauv hom Circular Consensus Sequencing (CCS) thiab txheeb xyuas cov kab ke Full-Length Non-Chimeric (FLNC), uas tom qab ntawd tau muab faib ua pawg rau hauv cov ntawv sau tsis rov ua dua. Kev tshuaj xyuas BUSCO tom qab ntawd ntsuas qhov tiav ntawm kev sib dhos transcriptome. Los ntawm cov ntawv sau transcriptome uas tau sib sau ua ke, ntau qhov kev tshuaj xyuas tau ua: kev sib txuas lwm txoj hauv kev, kev rov ua dua yooj yim (SSR), kev kwv yees ntawm lncRNA thiab cov noob caj noob ces sib xws, kev kwv yees ntawm cov noob caj noob ces tshiab, kev tshuaj xyuas tsev neeg noob caj noob ces, kev tshuaj xyuas cov ntaub ntawv sau, thiab kev sau ntawv ua haujlwm ntawm cov ntawv sau.